LOCUS       sequence-38705-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Nicholls SB, Chu J, Abbruzzese G, Tremblay KD, Hardy JA
  TITLE     Mechanism of a genetically encoded dark-to-bright reporter for 
            caspase activity.
  JOURNAL   J Biol Chem. 2011 Jul 15;286(28):24977-86. Epub 2011 May 10.
  PUBMED    21558267
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-07-15"; volume: "286(28)"; pages: "24977-86. 
            Epub 2011 May 10"
COMMENT     Sequence Label: pCAGFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    293..414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(330..349)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     384..403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(558..579)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     770..789
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcatggacg agctgtacaa ggatgaggtc gattttcagg ggccatgcaa tgattcgtca
       61 gaccctctgg tcgtggctgc ctccattatt ggtattctgc acctgatcct gtggattctg
      121 gatcgtctgt aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
      181 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      241 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      301 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      361 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
      421 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
      481 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      541 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      601 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      661 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      721 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      781 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      841 cacccgccgc gctt
//
