LOCUS       sequence-387082-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tp901 target with pUBQ10 switching from mTurq to mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: T1T/pUBQ10(mTurq->mScarlet)
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    311..335
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      486..921
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(1201..1220)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1212..2027
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1212..2027
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1285..1304)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2128..2716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2617..2636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2783..2805
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(2829..3081)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3121..3915)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3232..3251)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3842..3861
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3949..4128)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     4360..4382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4374..4391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4375..4391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4401..4420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4401..4419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(4429..5070)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(5079..5771)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(5354..5373)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5623..5641
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(5787..5807)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7970..7990
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8006..8008,8009..8011,8012..8725)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8051..8072)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8312..8331)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8659..8680
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcgttgttg ctaagaaatg cctgtaatgt cccacaaatg tagaaaatgg ttcgtacctt
       61 tgtccaagca ttgatatgtc tgatgagagg aaactgcaag atactgagct tggtttaacg
      121 aaggagaggc agtttcttcc ttccaaagca tttcatttga caatgccttg atcatcttaa
      181 gtagagtttc tgttgtggaa agtttgaaac tttgaagaaa cgactctcaa gtaaattgat
      241 gatcacaagt gaaagtgtat gttcgctgcc acagaatcag gggataacgc aggaaagaac
      301 atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga
      361 gatctaattc cggggatcgg aaatccagaa gcccgagagg ttgccgcctt tcgggctttt
      421 tctttttcaa aaaaaaaaat ttataaaacg atctgttgcg gccggccgcc gggttgtggg
      481 caaaggcgct cgacggtggg caaccgcttg cggttgtcca cgggcggagc cggtgcgcgt
      541 agcgcattgt ccacaagcca agggcgacca ataattgata tatatattca taattgaaaa
      601 gctaattgaa catactactt gctgtaacta cttgccggag cgaggggtgt ttgcaagctg
      661 ttgatctgaa agggctatta gcgttctcac gtgccttttt gattagcgat ttcacgtgac
      721 cttattagcg atttcacgta ctccgattag cgatttcacg taccctgatt agcgatttca
      781 cgtggatagt ttttggagcg ggccggaaag ccccgtgaat caaggctttg cggggcatta
      841 gcggtttcac gtggataact accctctatc cacaggcttc cggggataaa aaagcccgct
      901 cgacggcggg ctgttggatg gggatcgcct gaatcgcccc atcatccagc cagaaagtga
      961 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt
     1021 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag
     1081 caaaagttcg atttattcaa caaagccacg ttgtggctca aaatctctga tgttacattg
     1141 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     1201 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcaaggccg cgattaaatt
     1261 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1321 gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1381 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1441 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1501 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     1561 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1621 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1681 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1741 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     1801 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1861 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1921 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1981 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2041 attggttgta accatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2101 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2161 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2221 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2281 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2341 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2401 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2461 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2521 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2581 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2641 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2701 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2761 gccttttgct cacatgagat cttggcagga tatattgtgg tgtaacgtta tcagcttgca
     2821 tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
     2881 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
     2941 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
     3001 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
     3061 ttgccaaatg tttgaacgat ctgcttgact ctagctagag tccgaacccc agagtcccgc
     3121 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3181 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3241 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     3301 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc cgtgggtcac
     3361 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
     3421 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3481 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3541 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3601 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3661 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     3721 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     3781 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     3841 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     3901 atcttgttca atcatgcctc gatcgagttg agagtgaata tgagactcta attggatacc
     3961 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     4021 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     4081 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
     4141 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     4201 cgctcatgat cgataacatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     4261 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     4321 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     4381 cgacggccag tgagcgcgcg taatacgact cactataggg cggagcgatt gatgcatgtt
     4441 gtcaatcaat tggcaagtca taaaatgcat taaaaaatat tttcatactc aactacaaat
     4501 ccatgagtat aactataatt ataaagcaat gattagaatc tgacaaggat tctggaaaat
     4561 tacataaagg aaagttcata aatgtctaaa acacaagagg acatacttgt attcagtaac
     4621 atttgcagct tttctaggtc tgaaaatata tttgttgcct agtgaataag cataatggta
     4681 caactacaag tgttttactc ctcatattaa cttcggtcat tagaggccac gatttgacac
     4741 atttttactc aaaacaaaat gtttgcatat ctcttatatt ttcaaattca acacacaaca
     4801 aataagagaa aaaacaaata atattaattt gagaatgaac aaaaggacca tatcattcat
     4861 taactcttct ccatccattt ccatttcaca gttcgatagc gaaaaccgaa taaaaaacac
     4921 agtaaattac aagcacaaca aatggtacaa gaaaaacatg tttcccaatg ccataatact
     4981 cgaactcagt aggattctgg tgtgtgcgca atgaaactga tgcattgaac ttgacgaacg
     5041 ttgtcgaaac cgatgatacg aacgaaagct caagcttact tgtacagctc gtccatgccg
     5101 ccggtggagt ggcggccctc ggagcgttcg tactgttcca ccacggtgta gtcctcgttg
     5161 tgggaggtga tgtccaactt gcggtcgacg ttgtaggcgc cgggcatctg cacgggcttc
     5221 ttggccttgt aggtggtctt gaagtccgcc aggtagcggc cgccgtcctt caggcgcagg
     5281 gccatcttaa tgtcgccctt cagcacgccg tcctcggggt acaaccgctc ggtggacgct
     5341 tcccagccca ttgtcttctt ctgcattacg gggccgtcag gagggaagtt ggtgccgcgg
     5401 agcttcacct tgtagatcag ggtgccgtcc tccagggagg tgtcctgggt cacggtcacg
     5461 gcgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggactgc
     5521 ttatagtagt cggggatgtc ggcggggtgc ttggtgaagg ccctggagcc gtacatgaac
     5581 tgaggggaca ggatgtccca ggagaagggc agggggccac ccttggtcac cttcagcttg
     5641 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     5701 ccgttcatgg agccctccat gtgcaccttg aaccgcatga actccttgat cactgcctcg
     5761 cccttgctca caccactacc acttccgact ttcctcttct tctttggcat tgtttactat
     5821 gcgagttttt atttcgttta tttcaattaa ggtaactaaa aaactccttt ggagctcaac
     5881 aacaaacttt ccattcaccc atttatggat acacagtcta gctcaacaga gcttttaacc
     5941 caaattggta caatagaata caactttaga tcataattct caaaagaaag agattcctta
     6001 gctattctat ctgccactcc atttccttct cggcttgtat gcacaagcat aaaatcctca
     6061 aacttgctaa gtagatactt tatgtcttgg ataattggat tgagacttga caagcataac
     6121 tttcatgtaa ccaaagacac aagttgctga gaatccacct caaaaatgat cttcctataa
     6181 ttgaatcggg ataatgacag cacagcccat ctaagagcct ccacttctac ttccagcacg
     6241 cttcttactt ttaccacagc tcttgcacct aaccataaca ccttccctgt atgatcgcga
     6301 agcacccacc ctaagccaca ttttaatcct tctgttggcc atgccccatc aaagttgcac
     6361 ttaacccaag attgtggtgg agcttcccat gtttctcgtc tgtcccgacg gtgttgtggt
     6421 tggtgctttc cttacattct gagcctcttt ccttctaatc cactcatctg catcttcttg
     6481 tgtccttact aatacctcat tggttccaaa ttccctccct ttaagcacca gctcgtttct
     6541 gttcttccac agcctcccaa gtatccaagg gactaaagcc tccacattct tcagatcagg
     6601 atattcttgt ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc
     6661 ggataagttc ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt
     6721 tacattgtta ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat
     6781 ggaccaggcc ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt
     6841 caccaaacca cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta
     6901 tcctatgcaa atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat
     6961 ggataatttc acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt
     7021 ttataagccc acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag
     7081 cacgaagaat tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt
     7141 attgccaatt ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata
     7201 taaatcgtaa cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt
     7261 ggttgtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc
     7321 gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca
     7381 aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc
     7441 ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat
     7501 aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt
     7561 aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct
     7621 ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag
     7681 attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa
     7741 ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga
     7801 ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg
     7861 atcgaatttg tcgattaatc tgagtttttc tgattaacag tactgcaaaa aaagcaaaaa
     7921 gcatttacct tgattgagat gttaattgtg ttggcatacc taaacaatgc caaagaagaa
     7981 gaggaaagtc ggaagtggta gtggtatggt gagcaagggc gaggagctgt tcaccggggt
     8041 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     8101 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     8161 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     8221 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     8281 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     8341 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     8401 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcg acaacgtcta
     8461 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     8521 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8581 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     8641 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8701 cggcatggac gagctgtaca agtaataaat ctcgtctctg ttatgcttaa gaagttcaat
     8761 gtttcgtttc atgtaaaact ttggtggttt gtgttttggg gccttgtata atccctgatg
     8821 aataagtgtt ctactatgtt tccgttcctg ttatctcttt ctttctaatg acaagtcgaa
     8881 cttcttcttt atcatcgctt cgtttttatt atctgtgctt cttttgttta atacgcctgc
     8941 aaagtgactc gactctgttt agtgcagttc tgcgaaactt gtaaatagtc caattgttgg
     9001 cctctagtaa tagatgtagc gaaagtgttg agctgttggg ttctaaggat ggcttgaaca
     9061 tgttaatctt ttaggttctg agtatgatga aca
//
