LOCUS       sequence-387084-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1 target with pUBQ10 switching from mTurq to mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: T1B/pUBQ10(mTurq->mScarlet)
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    141..165
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      316..751
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(1031..1050)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1042..1857
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1042..1857
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1115..1134)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1958..2546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2447..2466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2613..2635
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(2659..2911)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2951..3745)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3062..3081)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3672..3691
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3779..3958)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     4190..4212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4204..4221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4205..4221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4231..4250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4231..4249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(4259..4900)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(4909..5601)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(5184..5203)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5453..5471
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(5617..5637)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5655..5702
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    7738..7787
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             7800..7820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7836..7838,7839..7841,7842..8555)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7881..7902)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8142..8161)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8489..8510
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcatcttaa gtagagtttc tgttgtggaa agtttgaaac tttgaagaaa cgactctcaa
       61 gtaaattgat gatcacaagt gaaagtgtat gttcgctgcc acagaatcag gggataacgc
      121 aggaaagaac atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt
      181 gtttgtttga gatctaattc cggggatcgg aaatccagaa gcccgagagg ttgccgcctt
      241 tcgggctttt tctttttcaa aaaaaaaaat ttataaaacg atctgttgcg gccggccgcc
      301 gggttgtggg caaaggcgct cgacggtggg caaccgcttg cggttgtcca cgggcggagc
      361 cggtgcgcgt agcgcattgt ccacaagcca agggcgacca ataattgata tatatattca
      421 taattgaaaa gctaattgaa catactactt gctgtaacta cttgccggag cgaggggtgt
      481 ttgcaagctg ttgatctgaa agggctatta gcgttctcac gtgccttttt gattagcgat
      541 ttcacgtgac cttattagcg atttcacgta ctccgattag cgatttcacg taccctgatt
      601 agcgatttca cgtggatagt ttttggagcg ggccggaaag ccccgtgaat caaggctttg
      661 cggggcatta gcggtttcac gtggataact accctctatc cacaggcttc cggggataaa
      721 aaagcccgct cgacggcggg ctgttggatg gggatcgcct gaatcgcccc atcatccagc
      781 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt
      841 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc
      901 ttcaactcag caaaagttcg atttattcaa caaagccacg ttgtggctca aaatctctga
      961 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1021 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcaaggccg
     1081 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1141 gggcaatcag gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt
     1201 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1261 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1321 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
     1381 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1441 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1501 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1561 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1621 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1681 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1741 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1801 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1861 gaattggtta attggttgta accatgacca aaatccctta acgtgagttt tcgttccact
     1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2101 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2581 ccttttgctg gccttttgct cacatgagat cttggcagga tatattgtgg tgtaacgtta
     2641 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     2701 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     2761 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     2821 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     2881 agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctagctagag tccgaacccc
     2941 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     3001 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     3061 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     3121 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     3181 cgtgggtcac gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt
     3241 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     3301 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     3361 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     3421 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     3481 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     3541 acgatagccg cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga
     3601 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     3661 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     3721 cgtgcaatcc atcttgttca atcatgcctc gatcgagttg agagtgaata tgagactcta
     3781 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     3841 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     3901 cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg
     3961 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     4021 cttaattctc cgctcatgat cgataacatt aacgcttaca atttccattc gccattcagg
     4081 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     4141 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     4201 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cggagcgatt
     4261 gatgcatgtt gtcaatcaat tggcaagtca taaaatgcat taaaaaatat tttcatactc
     4321 aactacaaat ccatgagtat aactataatt ataaagcaat gattagaatc tgacaaggat
     4381 tctggaaaat tacataaagg aaagttcata aatgtctaaa acacaagagg acatacttgt
     4441 attcagtaac atttgcagct tttctaggtc tgaaaatata tttgttgcct agtgaataag
     4501 cataatggta caactacaag tgttttactc ctcatattaa cttcggtcat tagaggccac
     4561 gatttgacac atttttactc aaaacaaaat gtttgcatat ctcttatatt ttcaaattca
     4621 acacacaaca aataagagaa aaaacaaata atattaattt gagaatgaac aaaaggacca
     4681 tatcattcat taactcttct ccatccattt ccatttcaca gttcgatagc gaaaaccgaa
     4741 taaaaaacac agtaaattac aagcacaaca aatggtacaa gaaaaacatg tttcccaatg
     4801 ccataatact cgaactcagt aggattctgg tgtgtgcgca atgaaactga tgcattgaac
     4861 ttgacgaacg ttgtcgaaac cgatgatacg aacgaaagct caagcttact tgtacagctc
     4921 gtccatgccg ccggtggagt ggcggccctc ggagcgttcg tactgttcca ccacggtgta
     4981 gtcctcgttg tgggaggtga tgtccaactt gcggtcgacg ttgtaggcgc cgggcatctg
     5041 cacgggcttc ttggccttgt aggtggtctt gaagtccgcc aggtagcggc cgccgtcctt
     5101 caggcgcagg gccatcttaa tgtcgccctt cagcacgccg tcctcggggt acaaccgctc
     5161 ggtggacgct tcccagccca ttgtcttctt ctgcattacg gggccgtcag gagggaagtt
     5221 ggtgccgcgg agcttcacct tgtagatcag ggtgccgtcc tccagggagg tgtcctgggt
     5281 cacggtcacg gcgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
     5341 gaaggactgc ttatagtagt cggggatgtc ggcggggtgc ttggtgaagg ccctggagcc
     5401 gtacatgaac tgaggggaca ggatgtccca ggagaagggc agggggccac ccttggtcac
     5461 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
     5521 gaactcgtgg ccgttcatgg agccctccat gtgcaccttg aaccgcatga actccttgat
     5581 cactgcctcg cccttgctca caccactacc acttccgact ttcctcttct tctttggcat
     5641 tgtttactat tcgtggtttg tctggtcaac caccgccgtc tcagtggtgt acggtacaaa
     5701 cccggagctc aacaacaaac tttccattca cccatttatg gatacacagt ctagctcaac
     5761 agagctttta acccaaattg gtacaataga atacaacttt agatcataat tctcaaaaga
     5821 aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg tatgcacaag
     5881 cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg gattgagact
     5941 tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca cctcaaaaat
     6001 gatcttccta taattgaatc gggataatga cagcacagcc catctaagag cctccacttc
     6061 tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata acaccttccc
     6121 tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg gccatgcccc
     6181 atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc gtctgtcccg
     6241 acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat
     6301 ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca
     6361 ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat
     6421 tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg
     6481 atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt
     6541 gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac
     6601 gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat taaataacaa
     6661 gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat
     6721 acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa
     6781 aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag
     6841 aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg
     6901 aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac
     6961 ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa
     7021 tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac
     7081 cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg
     7141 gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata
     7201 aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat
     7261 tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt
     7321 cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat
     7381 ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg
     7441 tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg
     7501 ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta
     7561 gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt
     7621 tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt
     7681 tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagtactgcc
     7741 cggatgatcc tgacgacgga gacggccgtc gtcgacaagc cggccgaacc taaacaatgc
     7801 caaagaagaa gaggaaagtc ggaagtggta gtggtatggt gagcaagggc gaggagctgt
     7861 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     7921 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     7981 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg
     8041 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     8101 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     8161 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     8221 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcg
     8281 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     8341 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     8401 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     8461 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     8521 ggatcactct cggcatggac gagctgtaca agtaataaat ctcgtctctg ttatgcttaa
     8581 gaagttcaat gtttcgtttc atgtaaaact ttggtggttt gtgttttggg gccttgtata
     8641 atccctgatg aataagtgtt ctactatgtt tccgttcctg ttatctcttt ctttctaatg
     8701 acaagtcgaa cttcttcttt atcatcgctt cgtttttatt atctgtgctt cttttgttta
     8761 atacgcctgc aaagtgactc gactctgttt agtgcagttc tgcgaaactt gtaaatagtc
     8821 caattgttgg cctctagtaa tagatgtagc gaaagtgttg agctgttggg ttctaaggat
     8881 ggcttgaaca tgttaatctt ttaggttctg agtatgatga acattcgttg ttgctaagaa
     8941 atgcctgtaa tgtcccacaa atgtagaaaa tggttcgtac ctttgtccaa gcattgatat
     9001 gtctgatgag aggaaactgc aagatactga gcttggttta acgaaggaga ggcagtttct
     9061 tccttccaaa gcatttcatt tgacaatgcc ttg
//
