LOCUS       sequence-387129-       14545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0658
FEATURES             Location/Qualifiers
     source          1..14545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10402..10990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10455..10475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10873..10884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11062..11295
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11373..11494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11410..11429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11464..11483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11534..11669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11689..11708)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11690..11708)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11718..11735)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11718..11734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11727..11749)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11876..12331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11963..11982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12173..12194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12357..12461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12462..12530,12531..13322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12680..12699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13493..14081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13982..14001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14235..14252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14369..14390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14405..14410,14411..14428,14429..14435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14443..14459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14448..14470
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14467..14483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14467..14483
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14502..14522
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14504..14522
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggct tcccctctga gggacgagga
     4141 ggaggaggag gaggagatgg tggtgtcgga ggaggaagaa gaggaggaag aagagggcga
     4201 cgaggaggag gaggaggagg tggaggcggc cgacgaggac gatgaggagg acgacgacga
     4261 gggagtactc gggcgcgggc cgggccacga ccggggccgc gaccgccaca gcccccccgg
     4321 ctgccacctc ttcccgccgc cgccgccgcc gccgccaccg ctgcccccgc cgccgccgcc
     4381 cccgccgcca gataaggatg acattcggct gctgccgtca gcattgggtg tgaagaagag
     4441 aaaacgagga cccaagaagc agaaggagaa caagccagga aaaccccgaa aacgcaagaa
     4501 gcgtgacagt gaggaggaat ttggttctga gcgagatgag taccgggaga agtcagagag
     4561 tgggggcagt gaatatggaa ccggaccggg tcggaaacga agaaggaagc accgagaaaa
     4621 aaaggagaag aagacaaagc ggcggaaaaa gggggaggga gatggggggc aaaagcaagt
     4681 ggaacagaag tcatcagcaa ctctgcttct gacctggggc ctggaggatg tggagcatgt
     4741 gttctctgag gaggattacc acacgctcac caactacaaa gccttcagcc agttcatgag
     4801 gcccctaatt gctaagaaga atcctaagat cccaatgtct aagatgatga ccatccttgg
     4861 ggccaaatgg agagagttca gtgccaacaa ccccttcaag gggtcagcag ctgctgtggc
     4921 ggcggcagcg gcagcagcag cagcagctgt agctgagcag gtgtcagctg ctgtctcgtc
     4981 ggccaccccc atagcaccct ccggaccccc cgcccttcca ccaccccctg ctgctgatat
     5041 ccagccccca cccatccgaa gagccaaaac caaagagggc aaaggtccag gccataagag
     5101 gcggagtaag agcccccgag tgcctgatgg acgcaagaag cttcggggaa agaaaatggc
     5161 accactcaaa ataaaactag ggcttctggg tggcaagagg aagaaaggag gctcgtatgt
     5221 ttttcagagc gacgaaggtc ctgaaccaga ggctgaggaa tcagacctgg acagtggcag
     5281 tgtccacagt gcctcaggcc ggcctgatgg ccctgtccgc accaagaaac taaagagagg
     5341 ccggccagga aggaagaaga agaaggtcct gggctgtcct gcagtggccg gggaggagga
     5401 ggttgatggc tacgagacgg atcaccagga ttactgtgag gtgtgccagc agggtgggga
     5461 aattattctg tgtgacacct gccctcgtgc ctaccacctc gtctgccttg atcctgagct
     5521 tgaccgggct ccagagggca aatggagctg ccctcactgt gagaaggagg gggtccagtg
     5581 ggaggccaag gaggaagaag aagaatacga agaggaggga gaggaagaag gggagaagga
     5641 ggaggaggat gatcacatgg agtactgccg cgtatgcaag gacggcgggg agctcctgtg
     5701 ctgtgacgcg tgcatctcct cctaccacat tcattgtcta aaccctcccc tgcctgacat
     5761 tcccaatggt gaatggctgt gtccccgatg cacatgcccc gtgctgaagg gtcgagtgca
     5821 gaagatccta cattggcggt ggggggagcc acctgtagca gtgccagccc ctcaacaggc
     5881 agatggaaat ccagatgtcc cacccccccg tcctcttcaa ggcagatcag agcgagagtt
     5941 ctttgtcaag tgggtaggac tatcctactg gcactgctcc tgggccaagg agcttcagct
     6001 ggaaatcttc catttggtta tgtatcgaaa ctaccagcgg aagaatgaca tggatgagcc
     6061 cccacccctg gactatggct ccggcgagga tgatgggaag agcgacaagc gtaaagtgaa
     6121 agacccgcac tatgctgaga tggaggagaa gtactatcgt tttggcatca agccagagtg
     6181 gatgaccgtc caccgcatca tcaaccacag tgtggataaa aaggggaatt accactatct
     6241 agtaaaatgg agggacttac catatgacca gtccacgtgg gaggaagatg aaatgaatat
     6301 ccctgaatac gaagaacata agcaaagcta ctggagacac cgagaactaa ttatggggga
     6361 agaccctgcc cagccccgca agtataagaa gaagaagaag gagctacagg gtgatgggcc
     6421 tcccagttct cccactaatg atcctaccgt gaaatatgag actcagccac ggtttatcac
     6481 agccactgga ggcaccctgc acatgtatca gttggaaggg ctgaactggc tacgcttctc
     6541 ctgggcccag ggcactgaca ccattctagc tgatgagatg gggctaggca agaccataca
     6601 aaccatcgtc ttcctctact cactctacaa ggagggccac acaaaaggtc ccttcctggt
     6661 gagtgcccca ctctctacca tcattaactg ggagcgggag ttccagatgt gggcacccaa
     6721 attctatgtg gtgacataca cgggtgacaa ggacagccgg gccatcattc gtgagaatga
     6781 attctccttt gaggacaatg ccatcaaagg gggcaagaaa gcttttaaga tgaagaggga
     6841 ggcacaggtg aagttccatg ttctcctgac atcgtatgag ctgatcacca ttgatcaggc
     6901 agcacttggt tccatccgct gggcctgtct tgtggtagat gaggcccatc gactcaagaa
     6961 caaccagtcc aagtttttca gggttctcaa tggttacaag atagatcata agttgctgct
     7021 gacaggaacc ccattgcaga ataatctgga ggagctcttc catctcctga acttcctcac
     7081 cccagagaga tttaacaact tggagggctt cctggaggag tttgctgaca tatccaaaga
     7141 ggaccagatc aagaaactgc atgatttgct ggggccacac atgctgcgga gactcaaggc
     7201 agatgtcttt aagaacatgc cagccaagac agagctcatc gttcgggtgg agctaagccc
     7261 catgcagaag aaatactaca aatacatcct gactcgaaat tttgaggcct tgaattcacg
     7321 aggtggtggg aaccaggtgt cgctgcttaa tatcatgatg gatcttaaga agtgctgcaa
     7381 ccatccatac ctttttcccg tggctgctat ggagtccccc aaactcccca gtggggctta
     7441 tgagggtggg gcacttatta agtcgtctgg gaagctcatg ctgctccaga agatgctgcg
     7501 aaagctgaag gagcaaggac accgagtgct catcttctcg cagatgacca aaatgttaga
     7561 cttgcttgag gacttcttag actatgaagg ctacaagtat gagcgcatcg atggtggtat
     7621 cacgggtgcc ctgaggcagg aggccatcga tcggtttaat gctcctgggg cccaacaatt
     7681 ctgcttcctc ctgtccaccc gagctggggg cctgggcatc aatctggcca ctgctgacac
     7741 tgtcatcatc tttgattctg actggaaccc ccataatgac atccaggcct ttagccgggc
     7801 tcatcggatt ggccaggcca acaaagtgat gatttaccgg tttgtgactc gcgcgtcagt
     7861 ggaagagcga atcacacaag tggccaagag aaagatgatg ctgacacacc tggttgtgcg
     7921 gcctgggctg ggctccaagg caggctccat gtccaagcag gagcttgacg acattctcaa
     7981 atttggcact gaagagctat tcaaggatga aaacgagggg gagaacaagg aggaggacag
     8041 cagtgtgatt cattatgaca atgaggccat cgctcggctg ttggaccgga accaggatgc
     8101 aactgaggac actgacgtgc agaacatgaa tgagtatctc agctccttca aggtggcaca
     8161 gtacgtcgtg cgggaagaag acaagattga ggaaattgag cgagagatca tcaagcagga
     8221 ggagaatgtg gaccctgact actgggagaa gctgctgagg catcactatg agcaacagca
     8281 ggaagaccta gcccggaatc taggcaaggg caagcgggtt cgcaagcaag ttaactacaa
     8341 tgatgctgct caggaagacc aagacaacca gtcagagtac tcggtgggtt cagaggagga
     8401 ggatgaagac ttcgatgaac gtcctgaagg gcgtagacag tcaaagaggc agctccggaa
     8461 tgagaaagat aagccactgc ctccactgct ggcccgagtc gggggcaaca ttgaggtgct
     8521 gggcttcaac acccgtcagc ggaaggcttt cctcaatgct gtgatgcgct gggggatgcc
     8581 accacaggat gccttcacca cacagtggct ggtgcgggac ctgaggggca agactgagaa
     8641 ggagtttaag gcctatgtgt ctttgttcat gcgccatctg tgtgagcctg gggcagacgg
     8701 ctctgaaacc tttgccgatg gggtccctcg ggagggactg agtcgccagc aggtgttgac
     8761 ccgcattgga gtcatgtctc tcgtcaaaaa gaaggtgcag gagtttgagc acatcaatgg
     8821 gcgttggtca atgccggaac tgatgcctga ccccagcgcc gattctaagc gctcctccag
     8881 agcctcctct cctaccaaaa cgtctcccac cactcctgag gcttctgcta ccaacagtcc
     8941 ctgcacctct aaacctgcta ctccagctcc aagtgagaaa ggagaaggca taaggacacc
     9001 tcttgagaag gaggaagctg aaaaccagga ggaaaagcca gagaagaaca gcagaattgg
     9061 ggagaagatg gagacagagg ctgatgcccc cagcccagcc ccatcacttg gggagcggct
     9121 ggagccaagg aagattcctc tagaggatga ggtgccaggg gtgcctggag agatggagcc
     9181 tgaacctggg taccgtgggg acagagagaa gtcagccaca gagtcgacgc caggagaaag
     9241 gggggaggag aagccgttgg atggacagga acacagggag aggccggagg gggaaacagg
     9301 ggatttgggc aagagagaag atgtaaaagg tgaccgggag cttcgaccag ggcctcgaga
     9361 tgagccacgg tccaatgggc gacgagagga aaagacagag aagccccggt tcatgttcaa
     9421 tatcgccgat ggtggcttca cagagcttca cacactgtgg cagaatgagg aacgggcagc
     9481 tatttcctcg gggaaactca atgagatctg gcacagaaga catgactatt ggcttctggc
     9541 tgggattgtc ctccatggct atgcacggtg gcaggacatc cagaatgatg ctcaatttgc
     9601 cattatcaac gagccattta aaactgaagc caataagggg aactttctgg agatgaaaaa
     9661 taagttcctg gcccggaggt tcaagctcct ggagcaggcg ctggtgattg aggagcagct
     9721 gcggcgggcg gcctacctga acctgtcgca ggagccggcg caccccgcca tggccctcca
     9781 cgcccgcttc gccgaggccg agtgcctggc cgagagccac cagcacctct ccaaggagtc
     9841 gctggcgggg aacaagccgg ccaacgccgt cctgcacaag gttctgaacc agctggagga
     9901 gttgctgagc gacatgaagg cggacgtgac ccgcctgcca gccacgctgt cccgaatacc
     9961 ccccatcgca gcccgccttc agatgtccga gcgcagcatc ctcagccggc tggccagcaa
    10021 gggcacggag cctcacccca caccggccta cccgccgggt ccctacgcta cacctccggg
    10081 gtacggggcg gccttcagcg ccgcacccgt aggggccctg gccgccgcag gcgccaatta
    10141 cagccagatg cctgcagggt ccttcatcac agccgccacc aacggccctc cagtgcttgt
    10201 gaagaaggag aaggaaatgg tgggggcatt ggtgtcagac gggctggatc ggaaggagcc
    10261 ccgagccggg gaggtgatct gtatagacga ctagtaatga actagtccgt aacttgaaag
    10321 tatttcgatt tcttggcttt atatatcttg tggaaaggac gatagactgt ggctgggaat
    10381 cggtccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
    10441 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    10501 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    10561 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    10621 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    10681 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    10741 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    10801 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    10861 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    10921 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    10981 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
    11041 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    11101 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    11161 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    11221 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    11281 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
    11341 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
    11401 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11461 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    11521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    11581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    11641 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    11701 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    11761 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    11821 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    11881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    12001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    12061 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    12121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    12181 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    12241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    12301 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    12361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    12421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    12481 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    12541 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    12601 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    12661 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    12721 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    12781 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    12841 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    12901 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    12961 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    13021 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    13081 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    13141 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    13201 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    13261 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    13321 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    13381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    13441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13621 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    14041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    14101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    14161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    14221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    14281 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    14341 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    14401 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    14461 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    14521 gggaacaaaa gctggagctg caagc
//
