LOCUS       sequence-38713-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, 
            Rahman N, Stuart DI, Owens RJ
  TITLE     A versatile ligation-independent cloning method suitable for 
            high-throughput expression screening applications.
  JOURNAL   Nucleic Acids Res. 2007  . 35(6):e45.
  PUBMED    17317681
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(6):e45."
COMMENT     Sequence Label: pOPINJ
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(56..165)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    181..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(205..224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(206..224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(266..285)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(347..369)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cctagtatag gggacatgtg atggtgatgg tgatgtgcca tggtatatct cctttgattg
       61 taaataaaat gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa
      121 ttcttattta actataatat attgtgttgg gttgaattaa aggtcccgga ttaactccgg
      181 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg gtccgatcca
      241 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
      301 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
      361 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc
      421 gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac
      481 agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccgaagg agaagggccg
      541 tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gc
//
