LOCUS       sequence-38714-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a LOVS1K, a light-controllable synthetic protein to 
            modulate local or global Ca2+ signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Pham E, Mills E, Truong K
  TITLE     A synthetic photoactivated protein to generate local or global 
            Ca(2+) signals.
  JOURNAL   Chem Biol. 2011 Jul 29;18(7):880-90.
  PUBMED    21802009
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2011-07-29"; volume: "18(7)"; pages: "880-90"
COMMENT     Sequence Label: LOVS1K
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        57..166
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             193..621
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 tacgactcac tataggggaa ttgtgagcgg ataacaattc cccggagtta atccgggacc
       61 tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca aatcatttgt
      121 atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag gagatatacc
      181 atggggacta gtttggctac tacacttgaa cgtattgaga agaactttgt cattactgac
      241 ccaagattgc cagataatcc cattatattc gcgtccgata gtttcttgca gttgacagaa
      301 tatagccgtg aagaaatttt gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat
      361 cgcgcgacag tgagaaaaat tagagatgcc atagataacc aaacagaggt cactgttcag
      421 ctgattaatt atacaaagag tggtaaaaag ttctggaacc tctttcactt gcagcctatg
      481 cgagatcaga agggagatgt ccagtacttt attggggttc agttggatgg aactgagcat
      541 gtccgagatg ctgccgagag agagggagtc atgctgatta agaaaactgc agaaaatatt
      601 gatgaggcgg caaaagaact tgctagtgga tcccagaacc gttactccaa ggagcacatg
      661 aagaagatga tgaaggactt ggaggggtta caccgagctg agcagagtct gcatgacctt
      721 caggaaaggc tgcacaaggc ccaggaggag caccgcacag tggaggtgga gaaggtccat
      781 ctggaaaaga agctgcgcga tgagatcaac cttgctaagc aggaagccca gcggctgaag
      841 gagctgcggg agggtactga gaatgagcgg agccgcc
//
