LOCUS       sequence-387143-        3519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SFXN1_STOP
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1076..1175)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1192..1211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1193..1211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1216..1232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1216..1232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1334..1353)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1345..2154
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1412..1431)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2301..2889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2790..2809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3043..3060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3219..3246)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3338..3424)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3473..3495
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3487..3504
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3488..3504
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgtc tggcgagctg
      121 ccccctaaca tcaatatcaa ggagccaaga tgggaccaga gcaccttcat cggccgcgcc
      181 aaccacttct ttaccgtgac agatccccgg aacatcctgc tgacaaatga gcagctggag
      241 agcgcccgga agatcgtgca cgactacaga cagggaatcg tgccaccagg actgaccgag
      301 aacgagctgt ggagagccaa gtacatctat gactccgcct tccacccaga tacaggcgag
      361 aagatgatcc tgatcggcag gatgtctgcc caggtgccca tgaatatgac catcacaggc
      421 tgcatgatga ccttttatcg caccacacct gccgtgctgt tctggcagtg gatcaaccag
      481 tcctttaatg ccgtggtgaa ctacacaaat aggtctggcg acgcccctct gaccgtgaac
      541 gagctgggca cagcctatgt gagcgccacc acaggagcag tggcaaccgc cctgggcctg
      601 aatgcactga caaagcacgt gtccccactg atcggccgct tcgtgccctt tgccgcagtg
      661 gccgccgcca actgtatcaa tatccccctg atgaggcagc gcgagctgaa ggtcggcatc
      721 cctgtgaccg atgagaacgg caataggctg ggagagagcg ccaacgcagc aaagcaggcc
      781 atcacacagg tggtggtgtc cagaatcctg atggcagcac ctggaatggc aatccctccc
      841 ttcatcatga ataccctgga gaagaaggcc ttcctgaagc ggtttccctg gatgtctgcc
      901 cctatccaag tgggcctggt gggcttctgc ctggtgtttg ccacccctct gtgctgtgcc
      961 ctgttcccac agaagagctc catgtctgtg acaagcctgg aggccgagct gcaggccaag
     1021 atccaggagt cccacccaga gctgcggaga gtgtacttta ataagggcct gtgagaccca
     1081 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1141 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1201 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1261 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1321 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1381 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1441 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1501 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1561 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1621 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1681 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1741 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1801 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1861 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1921 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1981 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2041 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2101 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2161 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2221 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3061 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3181 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3241 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3301 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3361 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3421 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3481 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
