LOCUS       sequence-38716-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Cup9p‐FLAG from the PCUP9 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Byrd C, Turner GC, Varshavsky A
  TITLE     The N-end rule pathway controls the import of peptides through 
            degradation of a transcriptional repressor.
  JOURNAL   EMBO J. 1998 Jan 2. 17(1):269-77.
  PUBMED    9427760
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            1998 Jan 2. 17(1):269-77."
COMMENT     Sequence Label: pCB210
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             368..391
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acctgataac ccctacccaa ctcaacaaga aaagagggag ttgttaatca aaactgggct
       61 aaccaagatt caactttcta attggttcat aaatgtgaga agacgcaaaa tatttagtga
      121 ttactatacc ctggtaaact caattcctaa tgacaacgcg aataataccc cagtggaacg
      181 agttcaaaac gtctcagcat atcataacac attatctgcc acaaataata ctatgtacga
      241 tgctacgtca acctgctcca cggattatga attatccaag agatttgctc atgcccccgt
      301 tacacgccgt aaaaaactaa ttgataggct ggaagaattg aaaaagctat ccaaccctga
      361 tatgaatgat tacaaagacg atgatgataa atgaggtcga ccgatgccct tgagagcctt
      421 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
      481 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
      541 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
      601 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
      661 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
      721 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
      781 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gac
//
