LOCUS       sequence-38721-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Petzold AM, Balciunas D, Sivasubbu S, Clark KJ, Bedell VM, Westcot 
            SE, Myers SR, Moulder GL, Thomas MJ, Ekker SC
  TITLE     Nicotine response genetics in the zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18662-7. Epub 2009 Oct 
            26.
  PUBMED    19858493
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-11-3"; volume: "106(44)"; pages: 
            "18662-7. Epub 2009 Oct 26"
COMMENT     Sequence Label: pGBT-PX
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    266..299
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(551..571)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacttgagta attttacttg attactgtac ttaagtatta tttttgggga tttttacttt
       61 acttgagtac aattaaaaat caatactttt acttttactt aattacattt ttttagaaaa
      121 aaaagtactt tttactcctt acaattttat ttacagtcaa aaagtactta ttttttggag
      181 atcacttcat tctattttcc cttgctatta ccaaaccaat tgaattgcgc tgatgcccag
      241 tttaatttgt agggataaca gggtaataac ttcgtataat gtatgctata cgaagttatc
      301 agcatgctta gctagtgtaa atgagaaaaa aaagaccggt aactgagcct aatcatttaa
      361 tctagcttta tttaaacatt ctaattaaac gttcgaattg atatatgcga gctgaaaatg
      421 taaaaagctt aaagatcaac tcaccttagg tttggggggg tttgttcatc gataagcttg
      481 atatcgaatt cctgcaggtc tggacattta tttgtatagt tcatccatgc catgtgtaat
      541 cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt
      601 cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc
      661 aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg
      721 tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt
      781 gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc
      841 aatacctttt aactcgattc tattaacaag ggtatcacct
//
