LOCUS       sequence-38722-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1252
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggaa taggaacttc
       61 ggcgcgccgg tcccgcaccg agaggaggcg gcgaggcacc agtcactttc gaagcggcgg
      121 ccactgggcg ctggcaccag agctgggcgg cggagctcag gagattccgc cgggcaccgt
      181 ctgccgcccg cctctgcgtg caagcctggc cgggacccag gcgcagctcg ctcttcttga
      241 gtgtcttgtc ttgctcactc agcccgcccg cgcggccgcc cgaccccgcg cgcctaggtc
      301 aggccagcca gcagcgtggc ccgcgtagct ccttcggccg aatgagcgct gcccggctcg
      361 cggccgggaa taaggctcca ggaggcgcgg aagctccgcc ccgcccggcg ccgccccctc
      421 cctcctcccg tgctgggaag ctcgcccggc tcgcggcgcc ggcagcgcag cccccgaccc
      481 cgcgggcgcg gcggaggact gggcgggagg agagggcggc gttcacaatt tttgggacca
      541 ggccgaggca gccgcggcct gattgtgggt agggctactg accggcaggt ggcggagagc
      601 ggttcctacc tttccccaaa cgcctgtcac tctgcagcct tgtgactgac tgctgagctg
      661 agttctgttc cgggcctggc ctcttgcttc atctaagccc cataccaggg gtctccatcc
      721 ctgtcaccct ctaagtttca
//
