LOCUS       sequence-387270-        8211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pSF11-wemiRFP670-T2A-HO1
FEATURES             Location/Qualifiers
     source          1..8211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3889..3942
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5781..5803)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5903..5922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6004..6022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6090..6194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6195..6263,6264..7055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6413..6432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7226..7814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7715..7734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7968..7985
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8102..8123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8138..8143,8144..8161,8162..8168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8176..8192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8181..8203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8200..8211,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8200..8211,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
      241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
      301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
      361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
      421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
      481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
      541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
      601 ttttttgaac ctttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
      661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
      721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
      781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
      841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
      901 aattctgaga tttcttagtg cattattcgg gcatacataa aaaaaaattt atcttaataa
      961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
     1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
     1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
     1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
     1201 aaaaaaaaaa cctcttttat taattttgtg ctccaaactt tcccacggta cgcaaatcat
     1261 gtaatatgta cttttaaaca ttatttttcc aatcaaaaat atccaacagc ttttccttac
     1321 aatttgaaaa tttttattaa aatttcaaaa aaattttttt tttttttaat ttcgaatttt
     1381 tttaaacttg ttgtaacttt tttccctgtc ggcgccaatt aacaaaatac gtagaatttt
     1441 gcgcttattg ttttgttagt taacaaattt ttgacaactt tcttatcagc tgagatttag
     1501 gtgtttcagt tcgcaatctg cgtctctcct tgctctcctc tattttaaaa cccccataac
     1561 tttgttcttg tgagagttat caaaaaattg tcaactaaca aaatatagag cacaaaaata
     1621 tgcacatttt gtatgttgac aattttttga tacctttttt agttttcgag atatcttagt
     1681 tttagtttag ctgtaccttt ttctggcaaa aaacttccaa atttcggcaa tttttcggca
     1741 aatttcccgg caaatttcgc caagtacatc gggctctcag acaagtggtc tgtgagtgga
     1801 agtcgataaa agtagtttac gtgctctccg tccccccgct tcttctccgt ctctgcttct
     1861 atttctccat tgactagtcc cactactttt tacggggcgg agggctctct tgcactccag
     1921 cttcttctct ttttgtgctc ttttttgccc gtatgtgggt gctcatttat ttttctttgg
     1981 ttttaaaata aattcggttt attaggctac tcgtaaaaat gaagaaaaaa cttcaaattc
     2041 aaacgacatt aaaaaaaaat ttaataaaaa ttttttgaat tgctcaaaag ccccacctat
     2101 atatatatat agctccgcct acttctcgca cagaccccgc ccactttttg actccgcccc
     2161 cttattggca aaagttcttg aaaaaaataa aaataaaaat tcaaattgaa acaatttttt
     2221 tttaatgaag tagttttttt ctaaattgat gaaaatttct aaacagttta caagcaccgc
     2281 ccactttttg actccgcccc tttgttggaa aaattcttgt aaaaaaaaat aaaaaataaa
     2341 agcttctctt tcaaacgatt tttagttggt ctttgtatga aaagtttttt tcctaaaata
     2401 gaaatgtctg aataatttgc aagctccgcc cttttcttac ccagactccg cccctttatt
     2461 gactaatttt tttttgtcca tttacctgaa gttcaaaaaa aaaatttaca gtttcataga
     2521 gtaataattt ttcaaaaatc tctttttcca atgtttaatg tgtcattata aacccgccca
     2581 gttttaagct ccgcccattt aggccccgcc catttatgac ccaaatattt ttttctctat
     2641 tcacccgtgg atttccctcc aacaagttac cccaaaaata aaaataaaaa ttcctgcagg
     2701 cgcccaccgg gatccggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
     2761 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg
     2821 tcgacggtac caaaaatggc cgagggatcc gtcgcccgtc aaccagacct tcttacctgc
     2881 gagcacgagg agatccacct tgccggatcc atccaaccac acggagccct tcttgtcgtc
     2941 tccgagcacg accaccgtgt catccaagcc tccgccaacg ccgccgagtt ccttaacctt
     3001 ggatccgtcc ttggagtccc acttgccgag atcgacggag accttcttat caaggtaagt
     3061 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagatcct tccacacctt
     3121 gacccaaccg ccgagggaat gccagtcgcc gtccgctgcc gcatcggaaa cccatccacc
     3181 gagtactgcg gacttatgca ccgcccacca gagggaggac ttatcatcga gcttgagcgt
     3241 gccggaccat ccatcgacct ttccggaacc cttgccccag cccttgagcg catccgtacc
     3301 gccggatccc ttcgtgccct ttgcgacgac accgtccttc ttttccagca atgcaccgga
     3361 tacgaccgtg tcatggtcta ccgcttcgac gagcaaggac acggacttgt cttctccgag
     3421 tgccacgtcc caggacttga gtcctacttc ggaaaccgct acccatcctc caccgtccca
     3481 caaatggccc gtcaacttta cgtccgtcaa cgtgtccgtg tccttgtcga cgtcacctac
     3541 caaccagtcc cacttgagcc acgtctttcc ccacttaccg gacgtgacct tgacatgtcc
     3601 ggatgcttcc ttcgttccat gtccccatgc caccttcaat tccttaaggt aagtttaaac
     3661 agttcggtac taactaacca tacatattta aattttcagg acatgggagt ccgtgccacc
     3721 cttgccgtct cccttgtcgt cggaggaaag ctttggggac ttgtcgtctg ccaccactac
     3781 cttccacgct tcatccgctt cgagcttcgt gccatctgca agcgtcttgc cgagcgcatc
     3841 gccacccgta tcaccgccct tgagtccgga tacgacatcg gatccggaga gggacgcgga
     3901 tcccttctta cctgcggaga tgttgaggaa aacccaggac caatggagcg cccacaacca
     3961 gattctatgc cacaagacct ttccgaggcc cttaaggagg ctaccaagga ggttcacacc
     4021 caagctgaga acgctgagtt catgcgcaac ttccaaaagg gacaagttac ccgtgatgga
     4081 ttcaagcttg tcatggcttc cctctaccac atctacgtcg ctcttgagga ggagatcgag
     4141 cgcaacaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     4201 gagtctccag ttttcgctcc agtttacttc ccagaagagc tccaccgtaa ggctgccctt
     4261 gagcaagacc ttgccttctg gtatggacca cgctggcaag aagttatccc atacactcca
     4321 gctatgcaac gctacgttaa gcgtcttcac gaggtcggac gtaccgagcc agagcttctt
     4381 gtcgctcacg cttacacccg ttaccttgga gacctttccg gaggacaagt ccttaaggta
     4441 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagaa gatcgctcaa
     4501 aaggctcttg accttccatc ttccggagag ggacttgcct tcttcacttt cccaaacatc
     4561 gcttctgcca ctaagttcaa gcaactttac cgctctcgta tgaactctct tgagatgacc
     4621 ccagctgtcc gccaacgtgt catcgaggag gctaaggtaa gtttaaacat gattttacta
     4681 actaactaat ctgatttaaa ttttcagacc gctttccttc ttaacatcca acttttcgaa
     4741 gagcttcaag agcttcttac ccacgacacc aaggaccaat ctccatcccg tgccccagga
     4801 cttcgtcaac gtgcctccaa caaggtccaa gattccgctc cagttgagac tccacgtgga
     4861 aagccaccac ttaacacccg ttcccaagcc ccacttcttc gttgggtcct taccctctcc
     4921 ttccttgtcg ccaccgtcgc tgtcggactt tacgccatgt aagagctccg catcggccgc
     4981 tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     5041 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     5101 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     5161 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     5221 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     5281 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     5341 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     5401 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     5461 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     5521 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     5581 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     5641 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     5701 aatataccaa acataactgt ttctactagt cggccgtacg ggccctttcg tctcgcgcgt
     5761 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5821 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5881 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5941 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaatta
     6001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6421 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6721 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6781 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7201 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7261 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7321 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7381 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7441 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7501 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7561 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7621 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7681 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7741 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7801 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7861 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7921 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7981 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8041 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8101 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8161 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc t
//
