LOCUS       sequence-387291-        6858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector to express nuclear localized dsRed-Express from CaMKIIa 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   de Solis CA, Hosek MP, Holehonnur R, Ho A, Banerjee A, Luong JA, 
            Jones LE, Chaturvedi D, Ploski JE
  TITLE     Adeno-associated viral serotypes differentially transduce inhibitory
            neurons within the rat amygdala.
  JOURNAL   Brain Res. 2017 Oct 1;1672:148-162. doi: 
            10.1016/j.brainres.2017.07.023. Epub 2017 Jul 29.
  PUBMED    28764932
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain 
            Res."; date: "2017-10-1"; volume: "1672:148-162. doi"; pages: " 
            10.1016/j.brainres.2017.07.023. Epub 2017 Jul 29"
COMMENT     Sequence Label: pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
FEATURES             Location/Qualifiers
     source          1..6858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        897..2185
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     intron          2265..2740
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     2822..2838
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2822..2838
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      2871..2880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2877..3551
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3007..3025)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3057..3077)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3275..3294
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3469..3492
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3567..3587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3591..3611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3615..3635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(3677..3695)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3678..3695)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3715..3732)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3721..3832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3976..4105
                     /label=AAV2 ITR
     rep_origin      4476..4989
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4563..4582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4773..4794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5471..5490)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5590..5612
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5650..5668)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5736..5840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5841..5909,5910..6701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6059..6078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gagcgagcgc
      781 gcagagaggg agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc
      841 catgctactt atctacgtag ccatgctcta gaggatcctt cgaaaagctt gtataacatt
      901 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
      961 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
     1021 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
     1081 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
     1141 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
     1201 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
     1261 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
     1321 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
     1381 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
     1441 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
     1501 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
     1561 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
     1621 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
     1681 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
     1741 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
     1801 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc
     1861 ccctcagtac atcaccctgg gggaacaggg tccacttgct cctgggccca cacagtcctg
     1921 cagtattgtg tatataaggc cagggcaaag aggagcaggt tttaaagtga aaggcaggca
     1981 ggtgttgggg aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat
     2041 ggggacctgg atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag
     2101 aagccccaag ctcgtcagtc aagccggttc tccgtttgca ctcaggagca cgggcaggcg
     2161 agtggcccct agttctgggg gcagcggggg atcccctacg gccggccacg cgttcgcgac
     2221 cgcgggctag cggcgcgccg agctcggatc ctgagaactt cagggtgagt ctatgggacc
     2281 cttgatgttt tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa
     2341 gtaacagggt acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat
     2401 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
     2461 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
     2521 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
     2581 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
     2641 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
     2701 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
     2761 ctgtgtgctg gcccatcact ttggcaaagc acgtgaattc gaagatctgg ccgcctcggc
     2821 ctctagaact agtggatccc ccgggctgca ggaattcgat gctaccggtc gccaccatgg
     2881 cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
     2941 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3001 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     3061 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     3121 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3181 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg
     3241 tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg
     3301 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     3361 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
     3421 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag ctggacatca
     3481 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc
     3541 acctgttcct gagatctcga gctgatccaa aaaagaagag aaaggtagat ccaaaaaaga
     3601 agagaaaggt agatccaaaa aagaagagaa aggtaggatc caccggatct agataacgta
     3661 cgggaaacgg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     3721 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3781 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3841 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3901 gggaagacaa tagcgcggcc gctctagagc atggctacgt agataagtag catggcgggt
     3961 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4021 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4081 cctcagtgag cgagcgagcg cgcagctggc gtaatagcga agaggcccgc accgatcgcc
     4141 cttcccaaca gttgcgcagc ctgaatggcg aatggcgatt ccgttgcaat ggctggcggt
     4201 aatattgttc tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt
     4261 gatgttatta ctaatcaaag aagtattgcg acaacggtta atttgcgtga tggacagact
     4321 cttttactcg gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc
     4381 ctgtctaaaa tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa
     4441 agcacgttat acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     4501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4921 gcttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc
     4981 aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt
     5041 ttgctccaga ctctcaggca atgacctgat agcctttgta gagacctctc aaaaatagct
     5101 accctctccg gcatgaattt atcagctaga acggttgaat atcatattga tggtgatttg
     5161 actgtctccg gcctttctca cccgtttgaa tctttaccta cacattactc aggcattgca
     5221 tttaaaatat atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc
     5281 gcaaaagtat tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag
     5341 gctttattgc ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgttgga
     5401 atcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5461 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5521 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5581 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5641 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5701 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5761 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5821 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5881 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5941 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6001 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6061 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6121 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6181 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6241 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6301 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6361 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6421 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6481 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6541 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6601 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6661 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6841 cagaccccgt agaaaaga
//
