LOCUS       sequence-387296-        2751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0030
FEATURES             Location/Qualifiers
     source          1..2751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(110..204)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(225..884)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     799..818
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1046..1064
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1068..1111)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1838..1895
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1919..1936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2090..2678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2170..2189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
       61 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
      121 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      181 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      241 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      301 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      361 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      421 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      481 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      541 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      601 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      661 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      721 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      781 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      841 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      901 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
      961 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     1021 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     1081 aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaaatg gtgaaacaag
     1141 aacgcaagat ccaaaccagc agcacaaaaa aggaaatgcc tttgtcatca tcaccatctt
     1201 cttcttcttc ttcatcttct tcctcgtctt cgtcttcgtg taagaacaag aacaagaaga
     1261 gtaagattaa gaagtacaaa ggagtgagga tgagaagttg gggatcatgg gtttctgaga
     1321 ttagggcacc aaatcaaaag acaaggattt ggttaggttc ttactcaaca gctgaagcag
     1381 ctgctagagc ttacgatgtt gcactcttat gtctcaaagg ccctcaagcc aatctcaact
     1441 tccctacttc ttcttcttct catcatcttc ttgataatct cttagatgaa aatacccttt
     1501 tgtcccccaa atccatccaa agagtagctg ctcaagctgc caactcattt aaccattttg
     1561 cccctacttc atcagccgtc tcgtcaccgt ccgatcatga tcatcaccat gatgatggga
     1621 tgcaatcttt gatgggatct tttgtggaca atcatgtgtc tttgatggat tcaacatctt
     1681 catggtatga tgatcataat gggatgttct tgtttgataa tggagctcca ttcaattact
     1741 ctcctcaact aaactcgacg acgatgctcg atgaatactt ctacgaagat gctgacattc
     1801 cgctttggag tttcaattaa gctttgagac cctgcagtcc ggcaaaaaag ggcaaggtgt
     1861 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
     1921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
     2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc a
//
