LOCUS       sequence-387299-        4584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0027
FEATURES             Location/Qualifiers
     source          1..4584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctcaaa gctcacaagc acatcatagt ttcctctgag ctttcgcatg agctcccaaa
     2101 gttaaaagtc acgtcatgta ctgagagcct cagtgtgttc gcttttgctg cagacgtgtc
     2161 gaggcattcg ctgaggtcag catcgcatga gacggacacc cattctctat cgtcgtctag
     2221 atacttgagc tcaaaggctg ccgtttcgag cttcagcctt ttggccactt gctgcttaag
     2281 ttccgtaatg cttaccgact ctggtgagat cttgaacctg atgatgtctt ctctgtacgt
     2341 tgcctttata ctcacgagct tatgttggag aattgttgga gaagctgtat cttggcaggt
     2401 taagtgtgcc caaaaatttg tcatgttttt agagctacta tccgaatcgt ccaaggccac
     2461 ttgtctggat ggttcttggt tgtgtgtagg aagccgaaaa cgcgtctcgc aaataggatt
     2521 cactttgcaa gaagcacgcg atgttgatga acctgcactg tcttctgcat ctctgttttc
     2581 gattttgaca tcttggagat tacttagggg agatgtagaa gttggagggc agttctttgc
     2641 taattggata tcgattggag ggatttggtg aggccaaggg cgaggagaga gggacgtcaa
     2701 gttgagtgat ccatcagcac cttgaaccga gtctataaca tgtttaagtc gtgtgagaga
     2761 tcggttcacc ttgttgattt ttcgagatgg ccaccgtgag atcccgtgtt gtcggcaaat
     2821 gcgtttcatt gttgttgggc aaactccgag gctctttgct gcatctttta gactaccagc
     2881 aaaatattgt tgaagcactt ctagactgat cgttttctct gttttcccac gcttcctttc
     2941 tgattttttc actgttttgg cctctggtat aggatcaagc gtttggaacc tctcaacccc
     3001 atcatcaact tcattattct ccataaagtc agagtttaag ctcaccttct gttcgggtgc
     3061 gttgagctca aggtaatcct tagagatttg tgctggatga tgtatcctga ttggttcaag
     3121 tttcgagtaa accattccgt cctccgaagc ttccacgact tcaagagaca tgttgttttc
     3181 acagagttca gtttcagaga caaccttgag actcgaatag tgctgcttca ttgtctgaag
     3241 cagagaaccc aatagacaat cttgctcact cttgtctgtg atggctggag gaagaaagaa
     3301 ttcaagaacg tactcatcat cccctgtata tgtgctcttt aagcagaccg cgaaacagct
     3361 cgtcaacttg aacatccgtg catagtgtac taatgggtag tcggttttgc agaaccgagt
     3421 aacatctcta cagaagcata gatttccaga ctgaaacgcc ctcccagcaa caccttgtcc
     3481 cttctgcaga tggtgttcag cacaggcgtc tcggaaaccc caaacatgag catcaacaac
     3541 ataaaccgct aaatcactag tagacataca gactttcccc atacagcttc catcgaagct
     3601 cgaacagctt ttcttaaaac cacctccaaa ggctagaaca ctgcgatgcc tgcaagggac
     3661 ccatgtctgg gctaaaggca gtttgtaggt ttcgcacacc actgtcagta tctccaaaat
     3721 ctcagctaac gcattttggc gaccctcgtt gcagatctga gtagtctcgt ggttcaatat
     3781 ttccgaagtt ttcagattca cggcctcaag cgctttacaa actttttcaa cctcaggagc
     3841 atagttaatc ttcggagaag tcataataag ctcgacaact cctacacaga gttgcctgga
     3901 tggttcaaaa acaggcaaag ccaaagttcc ctgaacatta tagtgcaagg cgtgaccaag
     3961 ccttgaaaac tctttgctgg agtaatactg cacattagga gtccactcag gtaacttttt
     4021 cctgaaaacg cgaccaggaa gcccgagctc tccgtcccgt tcaccatcaa gggaaaacat
     4081 atacgtcaat gaaaccatcc tgtattgatt aaggccatta ctattgggac caagaacaaa
     4141 tggctgtcct gaagttgtaa gaacatatcg accacggttt ttgacaggcg cccaaacctg
     4201 agcaagaaca tgttgttgtc cagtagattc cttgaagtat ctaagcgctt gagtcatctt
     4261 ggctttgatg gcacagtaac tatctggatt ctccaaaggc attatacccc atgacggaga
     4321 aggaacctga ttattctcct tggtcgtggt ttcagattca gaactagcga gtaagacgga
     4381 ataatcggtg aagcgagtgg gagctactgc ggcggagtaa agctcgccgc cgacatctcc
     4441 agaagtttcg gagaaggacc agagaggaga gaaaggttgt tcggaggaag agaaaatgac
     4501 aggagacttg aaatttgaag caaaagtgat ctgatctaac ggccaagaac cgctgagatc
     4561 caagtcgtca agttccattt gaga
//
