LOCUS       sequence-387300-        3073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0025
FEATURES             Location/Qualifiers
     source          1..3073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      197..291
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      474..1062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     963..982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1216..1233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1257..1314)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      2363..2406
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2410..2428)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2590..3073,1..176)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2656..2675)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
       61 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
      121 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg
      181 atatcgagct cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      241 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagcctc
      301 gagctgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      421 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      481 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      541 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      601 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      661 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      721 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      781 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      841 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      901 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      961 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1021 cgatttttgt gatgctcgtc aggggggcgg agcctgtgga aaaacgccag caacgcggcc
     1081 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1141 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1201 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gcataacgcg aagtaatctt
     1261 ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccttttttgc cggactgcag
     1321 ggtctcagga gcctataaaa tccctaatga gcacatcaac cttgaatctt ctctaataaa
     1381 ccttttttcc aaacacgcac taaaccaaaa attaacatct caagaggaaa ccatttaaaa
     1441 aaaaaacaga gttagattaa gatcaacaaa tatagttgaa aagaacatat gttaagcaac
     1501 attaccataa attcataatt agtagtgatt aaaacttaag aatactaaag tatgaatatg
     1561 aaaattgttg tatttttttg tagtatgtaa tatgcggaaa cttggatgtt atcctattca
     1621 ttgaaattca tatatgtatg tagttatcat tttttaaata tattaaaaaa ttaaaaagta
     1681 ataatactat aaacattcaa attatgaaat aaatatctca tactaatcac tgctaatttt
     1741 ttttccaatg tttctcttta gtatttctct ttagtatggg agtgacaaac ttaatatgaa
     1801 atctatctcc ctacatgcaa aaatcgaatc tcttttatta ttaatgaagt gtatggtcgg
     1861 tgtcaataac taatatgtca tattttttac agactgttca gatatgataa aaatagtttt
     1921 tttccaacca aatggttggt cttctgagtg tatttgaaaa cgacattgaa gaattaatat
     1981 attttttttt aattttagtt ttttatagta caaatattaa aacaaacaat cctaccatat
     2041 cataacattt gtaaataaca ttttaagttt tgttttgagt tttaattaat tttctatgac
     2101 aaaaaaatga agtcaataga ctaagtgaat catatagtat aaataaacac aatttaaata
     2161 gtttcaaata aatttagaaa gaataaaaca aatagaaatc agaaggtgtc tgtttcctcc
     2221 tcgcaacata cgatcaaaga gaaacaactt gaccctttac attgctcaag agctcatctc
     2281 ttccctctac aaaaatggcc gcacgtctcc aaccttctcc caactccttc ttccgccatc
     2341 atcaatgtga gaccgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc
     2401 tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2461 cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat
     2521 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     2581 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     2641 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     2701 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     2761 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
     2821 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2881 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2941 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     3001 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     3061 ttaaacgtgg cca
//
