LOCUS       sequence-387302-        3180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0022
FEATURES             Location/Qualifiers
     source          1..3180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      10..67
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(91..108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(262..850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(342..361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1033..1127)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1148..1807)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1722..1741
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1969..1987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1991..2034)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
       61 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      241 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
      301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1021 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1081 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1141 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1201 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1261 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1321 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1381 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1441 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1501 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1561 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1621 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1681 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1741 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1801 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1861 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     2041 tcggtctcag gagcaataat taaaccataa atggggccac tttcttggaa agttcgtatt
     2101 acgagttaac gcgttaaaac gcagctgtaa ttcgtaatct cctgatagtg atgataggta
     2161 cgtatatagt caatatataa aagaaaattg atatctcaca acctaaaaaa atactatagg
     2221 atattttagc atcactcctc gatttgctca tcaactggcg tccgtgtgta taatatatat
     2281 actctcctgg ttaattaatt cctagcaaaa ttgactccaa tcggtaattt attttagttt
     2341 gatagtttag ttcactcggt ttatattttg gacaaattct agaaagaatc taacacgtta
     2401 ataaacttgg agagaataaa tctaattgta aaatggaccc ttgtgaaatt aatgtttttt
     2461 gtcatttaaa ttgtagaata aaaaaatata catttttctt tgtaacaaga acatgcaatt
     2521 gataaaaatt tacgtgatcg cataattaat tgttgttttg tggttcctca ataaaaaaag
     2581 ccaatcaaaa tcattatacg tttatgtatt tttcctctaa ataccaagaa tattagttat
     2641 acatgattta ttttttataa gaaaatgcaa aaatagcaaa aggttaagat gaggccatgt
     2701 tcaggttcat tgttcgaact tcgaacaatt gggcaaagcc ttaacatgca tccatattcg
     2761 aataaataac taagctccaa agatttatgt tcactagttt cttttttttt ctttttttaa
     2821 tttgaatgga acaaaaatgt attaatgcca attgctagga taaaggaaaa attagaagaa
     2881 aaacacctgt catgcaccac aatgtcacat tcatacacaa gaaaccatac tatatttcat
     2941 ttgaccctat tttagattta tatcaataaa taatatttca tctcttcctc tcccataccc
     3001 ctcccaaatc aatccatctc tatatataat cttctcagct cacactaatc ctcatcaaaa
     3061 aatctctatc catccacaac aacaacaaaa actcaagaac aatatctcca cttgatcact
     3121 accacattgt ccaatcactc tacaaagcct gtacgtacac aacaacatta ccaatgcgag
//
