LOCUS       sequence-387304-        4938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0029
FEATURES             Location/Qualifiers
     source          1..4938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctcaaa gctcacaatt ctccagtgct ctcgcaggag ctccctagat ttgtcgttac
     2101 atcatgaact aagagcctta cgattttcgt gcgggaggat ctagggatct cgagacattc
     2161 ttgaagatca gcatcacaag ctattaaaac ccattcgtta tcatcgtcaa gatacttgat
     2221 atcgaacgtt cctgcatcaa ctttcagcct cttagccact tcatccttca attccattat
     2281 acctgaaccc gaggatattc tgaaccgtat tatgtcgtct ttgtaacttg ctttgattgt
     2341 taccgttctc atttctgagc ctgatggttc gcatgcaaca tttgcaatgg cctcttcctt
     2401 tgggggagcg tataagttgt tgttgctatt attatcattg ttcatccagt ttgtgtcttg
     2461 aaacttatcg tcaaatgctg cagtggggca gagatttctc agatcttttg aacttccagc
     2521 gtcttcaatg agcattcctc ggaaatggtc tatagaacca agaagcctgt tcggcattgc
     2581 gaaggaagct gcagatacat catgatctct agaataaggt ggaaaaagga gcccgggtga
     2641 tccaccaacc gtgaaaagcg gatcttgatt tgggactttc ggttcatcta attggttacc
     2701 gtcacatgag ccatgagagg ttggagtccc agcgctctca tccaccgttc gtgatcgctt
     2761 gtgaccattg cttggtggta actcaggcga accatttggc tcgttcggac tgtgatcact
     2821 tgaccaatgg ttaggtgaat tattggtgtt tggtagctca ggtggtttgg aaccattggg
     2881 tgagtttagt ggctgtgctg atgtttgacc gtgtgtccaa gggatggaac taacggccat
     2941 ggaagtcagg tcgaggcctc catcagtacc ttgaacagat tcgatgactc gtttcagctt
     3001 tgtgattgaa cgattcactt tcttgatctt cctcgatggc caccgcgaga ttccgtgttg
     3061 cctgcaaatt cgcttcattg tcgtcgggca aactcctagg ctctttgcag cgtctttgag
     3121 acttccagtg aaatactgct gaagtacatc tagactgatt gttttctcag tcttcccacg
     3181 ctttttctct gttttcttct tctccttaac cacaccgtta acagtggcca cgttgatttt
     3241 ctcatttact ggatcagaag actgaaccac aggctgagga atcaacatcg tctctgttgc
     3301 atttgactta aaaccagaaa agggaactcg aatggattct atttttgaat gaaccttctt
     3361 gtccggtaat gcttggatga tctcgaaaga caatttgtcg tcatcttcac caaagtcaac
     3421 cccagatgca accctcagac tctgaaaatg ttctttcatt gtcaccaaaa tagaacccaa
     3481 cagcaaatct tgctcttggt cgtctgttat actcgaagga agaaaaaatt caagaatgta
     3541 actgtcgtcg cccgtgtaag agctctggag agatattgca aaacaagtgg tcaacttgaa
     3601 catgagcgca taatggacta gtgggtactg cgttttgcag aacttggtga tgtctctgca
     3661 gaaacatgag ccaccattga gaaaagctcg tccagcgact ccctggcctt tctggagatg
     3721 gtgttcaaga caggcatctc taaagcccca gacatgagca tccacgacat agcaggccat
     3781 gtcggttgta gacatgcaga tttgacccat gcagctaccg tcaaagctgg tgcagttttt
     3841 ctttagaccg ccaccattgg caagaacgct cccatgctga catggaaccc aagtctgagc
     3901 gagaggcaag ttatgggttt cacatacaac tgtcagcact tcgagaatct cagcaagcgc
     3961 gttttggcga ctctcattgc atatctgtgt tgtttggtga tcaagtattt ccgagctttt
     4021 cagatttacc gcctcaaggg ctttgcaaac tttgtccact tcgggtgcat agtgaatctt
     4081 ctctgaggtc attataagtt ccacaacacc tatgcaggac tgaccagagg gattaaagac
     4141 aggcaaggcc agtgtaccac gcacgttgta gtgcaaggcg tgatcaagcc gcgagaattc
     4201 tttgctggaa tagtactgaa catttggagt ccattcaggc aatttctgac ggaaaactcg
     4261 acccgggagt ccgagctcta cgtcactttc actatccaca gaaaacatat atgtgagaga
     4321 gatcatcctg tattgattaa gcccattacc attaggatta agaacaaaag gttgacccaa
     4381 agtcgtcagc aaatcacgac cattctttct cacaggagcc cagacctgag ccaaaacgtg
     4441 ttgttcggtt gattctttga agtatcgaag cgcctgagtc atcctctctt tgatcacaca
     4501 gtagttgtct gtgttctctg gtgggactag gctcatcaat ggagacggaa actggaaact
     4561 gttatgcttc tctgttgtgt tctcagctgc tgatggagaa gagtaaggga ggaagagagg
     4621 atactcggcg agacggaaag aaggaacacc ggagacagag ctgatcttct catcatcgcc
     4681 accggaggtt gcgtggtgaa aaccatttcc tccaccgtcg gagaaagccc agagaggcga
     4741 gcaaggctgc tcagaggaag aggagacaaa aatcggagaa atcatgcgat tcgatgagga
     4801 taagtaaggg atttgatcta gtggccatga accgtcaaga tctaagtcgt caacatccat
     4861 tagaagctcc cttgacctcg aaggttgagt agtgacgccg tttctagcgg aattatcatc
     4921 gggctcgcac atttgaga
//
