LOCUS       sequence-387306-        4023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0112
FEATURES             Location/Qualifiers
     source          1..4023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..91
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(115..132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1057..1151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1172..1831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1746..1765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2015..2058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2061..2082
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     regulatory      2479..2488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
       61 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1081 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1141 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1201 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1261 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1321 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1381 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1441 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1501 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1561 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1621 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1681 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1741 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1801 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1861 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1921 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2041 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcgaat
     2101 ggcaaatcgc ggaggtgaat atctgtacga tgagttatgg aaattatgcg cgggacctct
     2161 tgttgatgtt cctcaagctc aagaaagagt ttattatttt cctcaaggtc acatggaaca
     2221 actcgaagcg tcaacgcaac aagtcgactt aaatacgatg aagcctcttt ttgttcttcc
     2281 tcctaagatt ctctgcaatg ttatgaacgt tagtcttcag gcggagaaag atacggatga
     2341 ggtctatgct cagattactt tgatccctgt tggaactgaa gttgatgaac ctatgagtcc
     2401 tgatccctct cctcctgagt tgcaaaggcc gaaagttcac tctttcagca aggttttgac
     2461 agcgtctgat acaagcaccc atggtggctt ttctgttcta aggaaacatg ccacggaatg
     2521 tcttcctccg ctggatatga ctcagcaaac cccgacccag gagttagtag ccgaagatgt
     2581 gcacggttat cagtggaaat tcaagcatat ttttagaggc caaccacgga ggcatctatt
     2641 gacgacaggg tggagcacct ttgttacatc aaagagattg gttgctgggg acacctttgt
     2701 attcctgaga ggggagaacg gagagttgcg agttggagtc agacgtgcta atcttcaaca
     2761 gagcagtatg ccttcatccg ttatatcaag tcatagtatg catctgggag tgcttgctac
     2821 tgcacgccat gctactcaaa cgaaaactat gttcattgta tactataaac caaggacaag
     2881 ccaattcatc attagcttga acaaatatct agaagccatg agcaataagt tctctgtagg
     2941 gatgagattt aagatgcgtt ttgagggaga ggattcccct gaaagaagat attctggcac
     3001 ggttattggt gtgaaagact gctcccctca ctggaaagac tcaaaatggc gatgcttaga
     3061 agttcattgg gatgagcctg catcgatttc aagacccaat aaggtttcac catgggaaat
     3121 tgaaccgttt gtaaattcag aaaacgttcc caaatcagtt atgctgaaga acaaaaggcc
     3181 ccgtcaagtt agtgaagtat ctgcacttga tgtaggcata acagcttcaa acctttggag
     3241 ctctgttttg acgcaacccc atgagtttgc acaatcgtgc atcacctcac agtggagttc
     3301 tcctcagcaa tgtcatcgtg atgcaaatga ggatgctaag aaatctgact ggctaaataa
     3361 ctcttactct gtgtcaaatg tagcaaaaga ctcaacactg aacgaccaaa tggtttcccc
     3421 agtcgagcag aagaagcctg agacaaccgc taattataga ttatttggaa ttgatctgat
     3481 gagttcctcc ctagcggttc ctgaggagaa aactgcaccc atgcgaccaa tcaacatatc
     3541 caaaccgact atggacagcc actcagaccc aaaatcagag atttcaaaag tatcagaaga
     3601 gaaaaagcag gaacctgcgg agggatcacc aaaagaggtc caaagcaagc aaagcagttc
     3661 tacaagaagc cgtaccaagg tgcagatgca aggcgtacct gtgggcaggg ctgtggattt
     3721 aaatgcgcta aaggggtaca acgagctcat agatgacatt gagaagctgt ttgacataaa
     3781 aggggaactg cggagtcgca atcaatggga aatagtgttc acagacgatg agggagatat
     3841 gatgcttgtc ggtgatgacc catggcctga gttctgcaac atggtgaaga gaatattcat
     3901 atggtcgaaa gaggaagtga agaaaatgac gcctgggaac caactccgga tgctgttaag
     3961 ggaagttgaa acaacactaa caacaacttc caaaacagat aatcattcca actaagcttt
     4021 gag
//
