LOCUS       sequence-387307-        2574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2574)
  TITLE     Patron Lab Plasmids
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEPOZ0CM0220
FEATURES             Location/Qualifiers
     source          1..2574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(90..749)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     664..683
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     911..929
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(933..976)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1526..1583
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1607..1624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1778..2366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1858..1877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2549..2574,1..69))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
       61 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      121 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      181 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      241 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      301 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      361 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      421 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      481 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
      541 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      601 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      661 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      721 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      781 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      841 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
      901 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
      961 cgctaaggat gatttctgga attcggtctc aaatgaatag tttcgagcca caaagccaag
     1021 actctttgca aagaaggttt catcaagaca acagcaccac acaacaacct cgtgacacca
     1081 cgacaccttt catacccaaa ccggcttcca aaaaccataa taatagcaac tccagctctg
     1141 gagcggccgg gagatcattc caaggctttg ggcttaacgt agaggacgat cttgtttcat
     1201 cggtggttcc tccggttacg gttgtgctag agggacgttc tatatgtcaa cgcataagcc
     1261 tagacaagca tgggagttat cagagcttgg cttcggctct aaggcaaatg tttgtcgatg
     1321 gagctgattc aacggacgat cttgatctgt caaacgccat tcctggccat cttattgctt
     1381 atgaagacat ggagaacgat ctccttcttg ccggagatct tacttggaag gactttgttc
     1441 gtgtagcgaa gagaattcga atcttgccgg tcaagggaaa cacaagacaa gttaaaagaa
     1501 acgagtgagc tttgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
     1561 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
     1621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg
     1801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2521 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggc
//
