LOCUS       sequence-387308-        3974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ0CM0139
FEATURES             Location/Qualifiers
     source          1..3974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      169..757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     658..677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     911..928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(952..1009)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     regulatory      1404..1413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      2959..3002
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3006..3024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3186..3845
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3252..3271)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3866..3960
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
       61 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
      121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      301 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      721 tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg cggccttttt
      781 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      841 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      901 gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta atcttttcgg
      961 ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac tgcagggtct
     1021 caaatggcaa atcgcggagg tgaatatctg tacgatgagt tatggaaatt atgcgcggga
     1081 cctcttgttg atgttcctca agctcaagaa agagtttatt attttcctca aggtcacatg
     1141 gaacaactcg aagcgtcaac gcaacaagtc gacttaaata cgatgaagcc tctttttgtt
     1201 cttcctccta agattctctg caatgttatg aacgttagtc ttcaggcgga gaaagatacg
     1261 gatgaggtct atgctcagat tactttgatc cctgttggaa ctgaagttga tgaacctatg
     1321 agtcctgatc cctctcctcc tgagttgcaa aggccgaaag ttcactcttt cagcaaggtt
     1381 ttgacagcgt ctgatacaag cacccatggt ggcttttctg ttctaaggaa acatgccacg
     1441 gaatgtcttc ctccgctgga tatgactcag caaaccccga cccaggagtt agtagccgaa
     1501 gatgtgcacg gttatcagtg gaaattcaag catattttta gaggccaacc acggaggcat
     1561 ctattgacga cagggtggag cacctttgtt acatcaaaga gattggttgc tggggacacc
     1621 tttgtattcc tgagagggga gaacggagag ttgcgagttg gagtcagacg tgctaatctt
     1681 caacagagca gtatgccttc atccgttata tcaagtcata gtatgcatct gggagtgctt
     1741 gctactgcac gccatgctac tcaaacgaaa actatgttca ttgtatacta taaaccaagg
     1801 acaagccaat tcatcattag cttgaacaaa tatctagaag ccatgagcaa taagttctct
     1861 gtagggatga gatttaagat gcgttttgag ggagaggatt cccctgaaag aagatattct
     1921 ggcacggtta ttggtgtgaa agactgctcc cctcactgga aagactcaaa atggcgatgc
     1981 ttagaagttc attgggatga gcctgcatcg atttcaagac ccaataaggt ttcaccatgg
     2041 gaaattgaac cgtttgtaaa ttcagaaaac gttcccaaat cagttatgct gaagaacaaa
     2101 aggccccgtc aagttagtga agtatctgca cttgatgtag gcataacagc ttcaaacctt
     2161 tggagctctg ttttgacgca accccatgag tttgcacaat cgtgcatcac ctcacagtgg
     2221 agttctcctc agcaatgtca tcgtgatgca aatgaggatg ctaagaaatc tgactggcta
     2281 aataactctt actctgtgtc aaatgtagca aaagactcaa cactgaacga ccaaatggtt
     2341 tccccagtcg agcagaagaa gcctgagaca accgctaatt atagattatt tggaattgat
     2401 ctgatgagtt cctccctagc ggttcctgag gagaaaactg cacccatgcg accaatcaac
     2461 atatccaaac cgactatgga cagccactca gacccaaaat cagagatttc aaaagtatca
     2521 gaagagaaaa agcaggaacc tgcggaggga tcaccaaaag aggtccaaag caagcaaagc
     2581 agttctacaa gaagccgtac caaggtgcag atgcaaggcg tacctgtggg cagggctgtg
     2641 gatttaaatg cgctaaaggg gtacaacgag ctcatagatg acattgagaa gctgtttgac
     2701 ataaaagggg aactgcggag tcgcaatcaa tgggaaatag tgttcacaga cgatgaggga
     2761 gatatgatgc ttgtcggtga tgacccatgg cctgagttct gcaacatggt gaagagaata
     2821 ttcatatggt cgaaagagga agtgaagaaa atgacgcctg ggaaccaact ccggatgctg
     2881 ttaagggaag ttgaaacaac actaacaaca acttccaaaa cagataatca ttccaacggt
     2941 tcgcgagacc gaattccaga aatcatcctt agcgaaagct aaggattttt tttatctgaa
     3001 attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3061 gacgtcaggt ggcactcgag ttgatcgggc acgtaagagg ttccaacttt caccataatg
     3121 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     3181 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     3241 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     3301 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     3361 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
     3421 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     3481 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     3541 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     3601 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     3661 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     3721 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3781 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     3841 cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa
     3901 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     3961 ccaagcctcg agct
//
