LOCUS       sequence-387309-        3867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0032
FEATURES             Location/Qualifiers
     source          1..3867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctcaaa tggcgagtgt tgaaggtgat gatgatttcg gaagttcttc gtcaaggtct
     2101 tatcaagatc aactatacac agagctatgg aaagtttgtg caggtccatt agtggaagtt
     2161 cctcgtgctc aagagagagt tttctacttc cctcagggtc acatggaaca acttgtggcg
     2221 tcaactaatc aaggaatcaa ttcagaagaa atacctgttt ttgatcttcc tccaaagata
     2281 ctttgtcgag ttcttgatgt cactttaaag gcggagcatg aaacagatga ggtttacgct
     2341 cagatcacat tacaaccaga ggaagatcaa agtgaaccaa caagtcttga tccacctatt
     2401 gttggaccaa ctaagcaaga gtttcattcg tttgttaaga ttttaacggc ttcagataca
     2461 agcactcatg gtggattctc tgttcttcgt aaacacgcca ctgaatgctt gccttctttg
     2521 gatatgacac aagctactcc tactcaagaa cttgtgacta gagatcttca tggctttgaa
     2581 tggaggttta agcatatatt cagaggacaa ccacggaggc atttgcttac tacgggttgg
     2641 agtacatttg tatcctcgaa aagacttgta gctggagatg cttttgtgtt cttgaggggt
     2701 gagaatgggg atttacgggt tggagtgaga cgattagctc ggcatcaaag cacaatgcct
     2761 acttcggtta tttcaagtca gagcatgcat ttgggagttc ttgctacagc ttctcatgct
     2821 gtgcgtacaa caacaatctt tgttgtcttt tacaagccta ggataagcca attcatagtt
     2881 ggggtgaaca agtatatgga agctataaag catggatttt ctctcggtac ccgattcaga
     2941 atgaggtttg aaggagaaga gtctcctgag agaatattta ctggtacgat tgtgggaagt
     3001 ggagatctat cttcacaatg gccagcttct aaatggaggt cattgcaggt acaatgggat
     3061 gagccaacaa cagttcaaag accagataaa gtctcaccat gggagataga gcctttcttg
     3121 gcaacttccc caatttcaac tcctgctcaa caaccacaat cgaaatgcaa gcggtcaaga
     3181 cccatcgagc catcagttaa aacaccagcc ccacctagtt tcttgtacag cctccctcag
     3241 agccaagatt ccattaatgc atcccttaaa ctgtttcaag atccatcact tgagagaatt
     3301 tcaggtggat actcctcaaa caacagcttc aaacccgaga ctcctcctcc tccaacgaat
     3361 tgtagctata ggttgtttgg atttgatctc acaagcaatt ctcctgctcc aatccctcaa
     3421 gacaagcaac cgatggatac ttgtggagct gccaagtgtc aagaacccat cactccaacc
     3481 tcaatgagtg agcagaagaa gcaacaaaca tcaagaagtc gaactaaagt gcaaatgcaa
     3541 ggcattgcgg ttggtcgtgc ggttgattta acactgttga aatcttacga tgaactgatt
     3601 gatgagcttg aggagatgtt tgagattcaa ggacagcttc ttgcccgaga caaatggatc
     3661 gttgtcttca ctgatgatga aggagatatg atgcttgctg gtgatgatcc gtggaatgag
     3721 ttttgcaaga tggcaaagaa gatatttata tattcgagcg atgaggttaa gaaaatgaca
     3781 acgaaactga agatttcttc gtcgttagag aatgaggaat atggtaatga atcattcgaa
     3841 aatcgtagta gggggtgagc tttgaga
//
