LOCUS       sequence-387312-        9538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1938
FEATURES             Location/Qualifiers
     source          1..9538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2476..3064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2529..2549)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2947..2958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3136..3369
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3447..3568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3484..3503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3538..3557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3608..3743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3763..3782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3764..3782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3792..3809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3792..3808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3801..3823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3950..4405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4037..4056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4247..4268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4431..4535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4536..4604,4605..5396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4754..4773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5567..6155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6056..6075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6309..6326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6443..6464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6479..6484,6485..6502,6503..6509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6522..6544
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6541..6557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6541..6557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6576..6596
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6578..6596
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6624..6850
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6851..7031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7078..7203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7696..7929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8114..8158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8389..8599
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8411..8607
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8458..8593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8639..9241
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8639..8658)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9135..9155
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9284..9401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gcaaaggtac gacgatctac cccattacgg gggcatggat ggagtaggca tcccctccac
     1261 gatgtatggg gacccgcatg cagccaggtc catgcagccg gtccaccacc tgaaccacgg
     1321 gcctcctctg cactcgcatc agtacccgca cacagctcat accaacgcca tggcccccag
     1381 catgggctcc tctgtcaatg acgctttaaa gagagataaa gatgccattt atggacaccc
     1441 cctcttccct ctcttagcac tgatttttga gaaatgtgaa ttagctactt gtaccccccg
     1501 cgagccgggg gtggcgggcg gggacgtctg ctcgtcagag tcattcaatg aagatatagc
     1561 cgtgttcgcc aaacagattc gcgcagaaaa acctctattt tcttctaatc cagaactgga
     1621 taacttgatg attcaagcca tacaagtatt aaggtttcat ctattggaat tagagaaggt
     1681 acacgaatta tgtgacaatt tctgccaccg gtatattagc tgtttgaaag ggaaaatgcc
     1741 tatcgatttg gtgatagacg atagagaagg aggatcaaaa tcagacagtg aagatataac
     1801 aagatcagca aatctaactg accagccctc ttggaacaga gatcatgatg acacggcatc
     1861 tactcgttca ggaggaaccc caggcccttc cagcggtggc cacacgtcac acagtgggga
     1921 caacagcagt gagcaaggtg atggcttgga caacagtgta gcttccccca gcacaggtga
     1981 cgatgatgac cctgataagg acaaaaagcg tcacaaaaag cgtggcatct ttcccaaagt
     2041 agccacaaat atcatgaggg cgtggctgtt ccagcatcta acacaccctt acccttctga
     2101 agaacagaaa aagcagttgg cacaagacac gggactcacc atccttcaag tgaacaattg
     2161 gtttattaat gcccggagaa gaatagtgca gcccatgata gaccagtcca accgagcagt
     2221 aagtcaagga acaccttata atcctgatgg acagcccatg ggaggtttcg taatggacgg
     2281 tcagcaacat atgggaatta gagcaccagg acctatgagt ggaatgggca tgaatatggg
     2341 catggagggg cagtggcact acatgtagta atgaactagt ccgtaacttg aaagtatttc
     2401 gatttcttgg ctttatatat cttgtggaaa ggacgagtac attcggcctg tgggctcatt
     2461 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2521 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2581 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2641 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2701 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2761 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2821 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2881 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2941 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3001 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3061 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3121 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3361 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3421 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3541 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3601 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3661 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3721 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3781 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     3961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4381 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4501 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4561 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4621 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4681 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4741 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4801 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4861 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4921 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4981 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5041 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5101 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5161 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5221 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5281 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5341 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5401 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5461 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5701 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5941 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6421 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6481 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6541 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6601 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6661 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6721 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6781 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     6841 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7021 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7081 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7141 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7201 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7261 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7321 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7381 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7441 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7501 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     7921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     7981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8341 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8401 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8461 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8521 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8581 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     8641 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8701 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8761 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8821 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     8881 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8941 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9001 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9061 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9121 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9181 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     9241 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9301 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9361 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9421 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     9481 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
