LOCUS       sequence-387313-        4583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0073
FEATURES             Location/Qualifiers
     source          1..4583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctcacg aacccaagca catcatagtt tcctctgagc tttcgcatga gctcccaaag
     2101 ttaaaagtca cgtcatgtac tgagagcctc agtgtgttcg cttttgctgc agacgtgtcg
     2161 aggcattcgc tgaggtcagc atcgcatgag acggacaccc attctctatc gtcgtctaga
     2221 tacttgagct caaaggctgc cgtttcgagc ttcagccttt tggccacttg ctgcttaagt
     2281 tccgtaatgc ttaccgactc tggtgagatc ttgaacctga tgatgtcttc tctgtacgtt
     2341 gcctttatac tcacgagctt atgttggaga attgttggag aagctgtatc ttggcaggtt
     2401 aagtgtgccc aaaaatttgt catgttttta gagctactat ccgaatcgtc caaggccact
     2461 tgtctggatg gttcttggtt gtgtgtagga agccgaaaac gcgtctcgca aataggattc
     2521 actttgcaag aagcacgcga tgttgatgaa cctgcactgt cttctgcatc tctgttttcg
     2581 attttgacat cttggagatt acttagggga gatgtagaag ttggagggca gttctttgct
     2641 aattggatat cgattggagg gatttggtga ggccaagggc gaggagagag ggacgtcaag
     2701 ttgagtgatc catcagcacc ttgaaccgag tctataacat gtttaagtcg tgtgagagat
     2761 cggttcacct tgttgatttt tcgagatggc caccgtgaga tcccgtgttg tcggcaaatg
     2821 cgtttcattg ttgttgggca aactccgagg ctctttgctg catcttttag actaccagca
     2881 aaatattgtt gaagcacttc tagactgatc gttttctctg ttttcccacg cttcctttct
     2941 gattttttca ctgttttggc ctctggtata ggatcaagcg tttggaacct ctcaacccca
     3001 tcatcaactt cattattctc cataaagtca gagtttaagc tcaccttctg ttcgggtgcg
     3061 ttgagctcaa ggtaatcctt agagatttgt gctggatgat gtatcctgat tggttcaagt
     3121 ttcgagtaaa ccattccgtc ctccgaagct tccacgactt caagagacat gttgttttca
     3181 cagagttcag tttcagagac aaccttgaga ctcgaatagt gctgcttcat tgtctgaagc
     3241 agagaaccca atagacaatc ttgctcactc ttgtctgtga tggctggagg aagaaagaat
     3301 tcaagaacgt actcatcatc ccctgtatat gtgctcttta agcagaccgc gaaacagctc
     3361 gtcaacttga acatccgtgc atagtgtact aatgggtagt cggttttgca gaaccgagta
     3421 acatctctac agaagcatag atttccagac tgaaacgccc tcccagcaac accttgtccc
     3481 ttctgcagat ggtgttcagc acaggcgtct cggaaacccc aaacatgagc atcaacaaca
     3541 taaaccgcta aatcactagt agacatacag actttcccca tacagcttcc atcgaagctc
     3601 gaacagcttt tcttaaaacc acctccaaag gctagaacac tgcgatgcct gcaagggacc
     3661 catgtctggg ctaaaggcag tttgtaggtt tcgcacacca ctgtcagtat ctccaaaatc
     3721 tcagctaacg cattttggcg accctcgttg cagatctgag tagtctcgtg gttcaatatt
     3781 tccgaagttt tcagattcac ggcctcaagc gctttacaaa ctttttcaac ctcaggagca
     3841 tagttaatct tcggagaagt cataataagc tcgacaactc ctacacagag ttgcctggat
     3901 ggttcaaaaa caggcaaagc caaagttccc tgaacattat agtgcaaggc gtgaccaagc
     3961 cttgaaaact ctttgctgga gtaatactgc acattaggag tccactcagg taactttttc
     4021 ctgaaaacgc gaccaggaag cccgagctct ccgtcccgtt caccatcaag ggaaaacata
     4081 tacgtcaatg aaaccatcct gtattgatta aggccattac tattgggacc aagaacaaat
     4141 ggctgtcctg aagttgtaag aacatatcga ccacggtttt tgacaggcgc ccaaacctga
     4201 gcaagaacat gttgttgtcc agtagattcc ttgaagtatc taagcgcttg agtcatcttg
     4261 gctttgatgg cacagtaact atctggattc tccaaaggca ttatacccca tgacggagaa
     4321 ggaacctgat tattctcctt ggtcgtggtt tcagattcag aactagcgag taagacggaa
     4381 taatcggtga agcgagtggg agctactgcg gcggagtaaa gctcgccgcc gacatctcca
     4441 gaagtttcgg agaaggacca gagaggagag aaaggttgtt cggaggaaga gaaaatgaca
     4501 ggagacttga aatttgaagc aaaagtgatc tgatctaacg gccaagaacc gctgagatcc
     4561 aagtcgtcaa gttccatttg aga
//
