LOCUS       sequence-387314-        3866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ0CM0138
FEATURES             Location/Qualifiers
     source          1..3866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      158..746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     647..666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     900..917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(941..998)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      2840..2883
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2887..2905)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3067..3726
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3133..3152)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3747..3841
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
       61 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      721 cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      901 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
      961 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagggtctc aaatggcgag
     1021 tgttgaaggt gatgatgatt tcggaagttc ttcgtcaagg tcttatcaag atcaactata
     1081 cacagagcta tggaaagttt gtgcaggtcc attagtggaa gttcctcgtg ctcaagagag
     1141 agttttctac ttccctcagg gtcacatgga acaacttgtg gcgtcaacta atcaaggaat
     1201 caattcagaa gaaatacctg tttttgatct tcctccaaag atactttgtc gagttcttga
     1261 tgtcacttta aaggcggagc atgaaacaga tgaggtttac gctcagatca cattacaacc
     1321 agaggaagat caaagtgaac caacaagtct tgatccacct attgttggac caactaagca
     1381 agagtttcat tcgtttgtta agattttaac ggcttcagat acaagcactc atggtggatt
     1441 ctctgttctt cgtaaacacg ccactgaatg cttgccttct ttggatatga cacaagctac
     1501 tcctactcaa gaacttgtga ctagagatct tcatggcttt gaatggaggt ttaagcatat
     1561 attcagagga caaccacgga ggcatttgct tactacgggt tggagtacat ttgtatcctc
     1621 gaaaagactt gtagctggag atgcttttgt gttcttgagg ggtgagaatg gggatttacg
     1681 ggttggagtg agacgattag ctcggcatca aagcacaatg cctacttcgg ttatttcaag
     1741 tcagagcatg catttgggag ttcttgctac agcttctcat gctgtgcgta caacaacaat
     1801 ctttgttgtc ttttacaagc ctaggataag ccaattcata gttggggtga acaagtatat
     1861 ggaagctata aagcatggat tttctctcgg tacccgattc agaatgaggt ttgaaggaga
     1921 agagtctcct gagagaatat ttactggtac gattgtggga agtggagatc tatcttcaca
     1981 atggccagct tctaaatgga ggtcattgca ggtacaatgg gatgagccaa caacagttca
     2041 aagaccagat aaagtctcac catgggagat agagcctttc ttggcaactt ccccaatttc
     2101 aactcctgct caacaaccac aatcgaaatg caagcggtca agacccatcg agccatcagt
     2161 taaaacacca gccccaccta gtttcttgta cagcctccct cagagccaag attccattaa
     2221 tgcatccctt aaactgtttc aagatccatc acttgagaga atttcaggtg gatactcctc
     2281 aaacaacagc ttcaaacccg agactcctcc tcctccaacg aattgtagct ataggttgtt
     2341 tggatttgat ctcacaagca attctcctgc tccaatccct caagacaagc aaccgatgga
     2401 tacttgtgga gctgccaagt gtcaagaacc catcactcca acctcaatga gtgagcagaa
     2461 gaagcaacaa acatcaagaa gtcgaactaa agtgcaaatg caaggcattg cggttggtcg
     2521 tgcggttgat ttaacactgt tgaaatctta cgatgaactg attgatgagc ttgaggagat
     2581 gtttgagatt caaggacagc ttcttgcccg agacaaatgg atcgttgtct tcactgatga
     2641 tgaaggagat atgatgcttg ctggtgatga tccgtggaat gagttttgca agatggcaaa
     2701 gaagatattt atatattcga gcgatgaggt taagaaaatg acaacgaaac tgaagatttc
     2761 ttcgtcgtta gagaatgagg aatatggtaa tgaatcattc gaaaatcgta gtaggggggg
     2821 ttcgcgagac cgaattccag aaatcatcct tagcgaaagc taaggatttt ttttatctga
     2881 aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2941 agacgtcagg tggcactcga gttgatcggg cacgtaagag gttccaactt tcaccataat
     3001 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     3061 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     3121 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     3181 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     3241 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa
     3301 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     3361 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     3421 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     3481 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     3541 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     3601 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     3661 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     3721 gcgtaatttg atatcgagct cgcttggact cctgttgata gatccagtaa tgacctcaga
     3781 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
     3841 tccaagcctc gagctgtcag accaag
//
