LOCUS       sequence-387317-        4937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0074
FEATURES             Location/Qualifiers
     source          1..4937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctcacg aacccaattc tccagtgctc tcgcaggagc tccctagatt tgtcgttaca
     2101 tcatgaacta agagccttac gattttcgtg cgggaggatc tagggatctc gagacattct
     2161 tgaagatcag catcacaagc tattaaaacc cattcgttat catcgtcaag atacttgata
     2221 tcgaacgttc ctgcatcaac tttcagcctc ttagccactt catccttcaa ttccattata
     2281 cctgaacccg aggatattct gaaccgtatt atgtcgtctt tgtaacttgc tttgattgtt
     2341 accgttctca tttctgagcc tgatggttcg catgcaacat ttgcaatggc ctcttccttt
     2401 gggggagcgt ataagttgtt gttgctatta ttatcattgt tcatccagtt tgtgtcttga
     2461 aacttatcgt caaatgctgc agtggggcag agatttctca gatcttttga acttccagcg
     2521 tcttcaatga gcattcctcg gaaatggtct atagaaccaa gaagcctgtt cggcattgcg
     2581 aaggaagctg cagatacatc atgatctcta gaataaggtg gaaaaaggag cccgggtgat
     2641 ccaccaaccg tgaaaagcgg atcttgattt gggactttcg gttcatctaa ttggttaccg
     2701 tcacatgagc catgagaggt tggagtccca gcgctctcat ccaccgttcg tgatcgcttg
     2761 tgaccattgc ttggtggtaa ctcaggcgaa ccatttggct cgttcggact gtgatcactt
     2821 gaccaatggt taggtgaatt attggtgttt ggtagctcag gtggtttgga accattgggt
     2881 gagtttagtg gctgtgctga tgtttgaccg tgtgtccaag ggatggaact aacggccatg
     2941 gaagtcaggt cgaggcctcc atcagtacct tgaacagatt cgatgactcg tttcagcttt
     3001 gtgattgaac gattcacttt cttgatcttc ctcgatggcc accgcgagat tccgtgttgc
     3061 ctgcaaattc gcttcattgt cgtcgggcaa actcctaggc tctttgcagc gtctttgaga
     3121 cttccagtga aatactgctg aagtacatct agactgattg ttttctcagt cttcccacgc
     3181 tttttctctg ttttcttctt ctccttaacc acaccgttaa cagtggccac gttgattttc
     3241 tcatttactg gatcagaaga ctgaaccaca ggctgaggaa tcaacatcgt ctctgttgca
     3301 tttgacttaa aaccagaaaa gggaactcga atggattcta tttttgaatg aaccttcttg
     3361 tccggtaatg cttggatgat ctcgaaagac aatttgtcgt catcttcacc aaagtcaacc
     3421 ccagatgcaa ccctcagact ctgaaaatgt tctttcattg tcaccaaaat agaacccaac
     3481 agcaaatctt gctcttggtc gtctgttata ctcgaaggaa gaaaaaattc aagaatgtaa
     3541 ctgtcgtcgc ccgtgtaaga gctctggaga gatattgcaa aacaagtggt caacttgaac
     3601 atgagcgcat aatggactag tgggtactgc gttttgcaga acttggtgat gtctctgcag
     3661 aaacatgagc caccattgag aaaagctcgt ccagcgactc cctggccttt ctggagatgg
     3721 tgttcaagac aggcatctct aaagccccag acatgagcat ccacgacata gcaggccatg
     3781 tcggttgtag acatgcagat ttgacccatg cagctaccgt caaagctggt gcagtttttc
     3841 tttagaccgc caccattggc aagaacgctc ccatgctgac atggaaccca agtctgagcg
     3901 agaggcaagt tatgggtttc acatacaact gtcagcactt cgagaatctc agcaagcgcg
     3961 ttttggcgac tctcattgca tatctgtgtt gtttggtgat caagtatttc cgagcttttc
     4021 agatttaccg cctcaagggc tttgcaaact ttgtccactt cgggtgcata gtgaatcttc
     4081 tctgaggtca ttataagttc cacaacacct atgcaggact gaccagaggg attaaagaca
     4141 ggcaaggcca gtgtaccacg cacgttgtag tgcaaggcgt gatcaagccg cgagaattct
     4201 ttgctggaat agtactgaac atttggagtc cattcaggca atttctgacg gaaaactcga
     4261 cccgggagtc cgagctctac gtcactttca ctatccacag aaaacatata tgtgagagag
     4321 atcatcctgt attgattaag cccattacca ttaggattaa gaacaaaagg ttgacccaaa
     4381 gtcgtcagca aatcacgacc attctttctc acaggagccc agacctgagc caaaacgtgt
     4441 tgttcggttg attctttgaa gtatcgaagc gcctgagtca tcctctcttt gatcacacag
     4501 tagttgtctg tgttctctgg tgggactagg ctcatcaatg gagacggaaa ctggaaactg
     4561 ttatgcttct ctgttgtgtt ctcagctgct gatggagaag agtaagggag gaagagagga
     4621 tactcggcga gacggaaaga aggaacaccg gagacagagc tgatcttctc atcatcgcca
     4681 ccggaggttg cgtggtgaaa accatttcct ccaccgtcgg agaaagccca gagaggcgag
     4741 caaggctgct cagaggaaga ggagacaaaa atcggagaaa tcatgcgatt cgatgaggat
     4801 aagtaaggga tttgatctag tggccatgaa ccgtcaagat ctaagtcgtc aacatccatt
     4861 agaagctccc ttgacctcga aggttgagta gtgacgccgt ttctagcgga attatcatcg
     4921 ggctcgcaca tttgaga
//
