LOCUS       sequence-387337-        8115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CoA-Snifit in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Xue L, Schnacke P, Frei MS, Koch B, Hiblot J, Wombacher R, Fabritz 
            S, Johnsson K
  TITLE     Probing coenzyme A homeostasis with semisynthetic biosensors.
  JOURNAL   Nat Chem Biol. 2022 Oct 31. doi: 10.1038/s41589-022-01172-7.
  PUBMED    36316571
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Oct 31. doi: 10.1038/s41589-022-01172-7."
COMMENT     Sequence Label: pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)
FEATURES             Location/Qualifiers
     source          1..8115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        296..675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        676..879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     830..850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    881..899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    902..920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     930..954
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1057..1131
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1144..1215
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1234..1305
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1318..1389
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1420..2304
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             join(2410..2412,2413..2925)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVE"
     primer_bind     complement(2452..2473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3060..3081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4128..4145)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4134..4358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4491..4510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4642..4689
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4697..5719
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4697..5719
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5849..5970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5886..5905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5940..5959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6019..6035)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6019..6035)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6032..6054)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6043..6059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6067..6073,6074..6091,6092..6097))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6112..6133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6250..6267)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6421..7009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6501..6520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7180..7971,7972..8040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7803..7822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8041..8115,1..30))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
       61 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
      121 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      181 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      241 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      301 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      361 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      421 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      601 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      661 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      721 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      781 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      841 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
      901 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
      961 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     1021 actctagcgt ttaaacttaa gcttggtacc gccaccatgt ctgtgctgac acctctgctg
     1081 ctgagaggcc tgacaggctc tgctagaagg ctgcctgtgc ctagagccaa gatccactct
     1141 ctgagcgtgc tgaccccact gctccttagg ggactgactg gatctgctcg gagactgcca
     1201 gtgcctcggg ccaaaattca tagcctcggc ggatccgtcc tgactccact ccttctgaga
     1261 ggactcaccg gcagtgctag acgcctgcca gttccaaggg ctaagatcca cagtctgtca
     1321 gtgctcactc ccctgttgct cagagggctg actggaagtg caagacgact ccccgtgcca
     1381 cgcgccaaga ttcattctct tggaggctct ggcggcagcg agatcggaac cggctttcct
     1441 ttcgaccctc actacgtgga agtgctgggc gagagaatgc actatgtgga cgtgggcccc
     1501 agagatggaa cccctgtgct gtttctgcac ggcaacccta ccagcagcta cgtgtggcgg
     1561 aacatcatcc ctcacgtggc ccctacacac agatgtatcg cccctgacct gatcggcatg
     1621 ggcaagagcg ataagcccga cctgggctac ttcttcgacg accacgtgcg gttcatggac
     1681 gcctttattg aggccctcgg cctggaagag gtggtgctgg ttattcacga ttggggcagc
     1741 gccctgggat tccactgggc caagagaaac cccgagagag tgaagggaat cgccttcatg
     1801 gaattcatca ggcccattcc tacctgggac gagtggcctg agttcgccag agagacattc
     1861 caggccttca gaaccaccga cgtgggcaga aagctgatca tcgaccagaa cgtgttcatc
     1921 gagggcaccc tgcctatggg agtcgttaga cctctgaccg aggtggaaat ggaccactac
     1981 agagagccct ttctgaaccc cgtggaccgg gaacctcttt ggagattccc caacgagctg
     2041 cccattgccg gcgagcctgc taatattgtg gccctggtgg aagagtacat ggactggctg
     2101 catcagagcc ccgtgcctaa gctgctgttt tggggaacac ccggcgtgct gattcctcct
     2161 gctgaagctg ccagactggc caagagcctg cctaattgca aggccgtgga tatcggccct
     2221 ggcctgaatc tgctgcaaga ggacaacccc gatctgatcg gctccgagat tgccagatgg
     2281 ctgagcaccc tggaaatcag cggaggatct ctggctgaag ccgccgctaa agaagccgct
     2341 gcaaaagagg ctgccgccaa agaggcagca gcaaaagaag cagccgctaa ggctgctgct
     2401 ggcggatctg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2461 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     2521 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2581 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2641 atgaagcgcc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2701 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     2761 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2821 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcaccgc cgacaagcag
     2881 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
     2941 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3001 aaccactacc tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac
     3061 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcggatc ccctcaccac
     3121 gtgttcgtgt ccgacttcgt ggacttcagc atctacgtgg acgcccctga ggatctgctg
     3181 cagacctggt acatcaaccg gtttctgaag ttcagagagg gcgcctacac agaccccgac
     3241 agctacttcc acaactacgc caagctgacc aaagaggaag ccatcaagac cgccatgacc
     3301 tggtggaaag agatcaactg gctgaacctg aagcagaaca tcctgcctac cagagagcgg
     3361 gccagcctga tcctgaccaa atctgccaat cacgccgtgg aagaagtgcg gctgagaaaa
     3421 ggatctggcg gcaccggcac accctacctg cagttcgata gaaaccagtg ggccgctctg
     3481 agagacagcg tgccaatgac actgagcgag gacgagatcg ccagactgaa gagcatcaat
     3541 gaggacctga gcctggaaga ggtggccgag atttacctgc ccctgagccg gctgctgaac
     3601 ttctacatca gcagcaacct gcggagacag gccgtgctgg aacagttcct gggaacaaac
     3661 ggccagcgga tcccctacat catctctatc gccggatctg tggccgtggg caagagcaca
     3721 acagctagag ttctgcaggc cctgctgagc agatggcccg agcatagaag agtggaactg
     3781 attaccaccg ccggctttct gcaccccaac caggtgctga aagaacgggg cctgatgaag
     3841 aagaagggct tccccgagag ctacgacatg caccggctgg tcaagtttgt gtccgacctg
     3901 aagtccggcg tgcccaatgt gacagcccct gtgtacagcc acctgatcta cgacgtgatc
     3961 cccgacggcg ataagaccgt ggtgcagccc gatatcctga ttctggaagg cctgaacgtg
     4021 ctgcagagcg gcatggacta tcctcatgat ggcgctccag gattctcctc aatttccgct
     4081 taagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4141 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4201 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4261 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4321 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4381 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4441 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4501 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4561 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4621 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     4681 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     4741 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     4801 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     4861 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     4921 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     4981 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     5041 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     5101 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     5161 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     5221 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     5281 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     5341 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     5401 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     5461 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     5521 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     5581 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     5641 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     5701 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     5761 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5821 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5881 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5941 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6001 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6061 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6841 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8101 catatttgaa tgtat
//
