LOCUS       sequence-387341-        8048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CoA-Snifit in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Xue L, Schnacke P, Frei MS, Koch B, Hiblot J, Wombacher R, Fabritz 
            S, Johnsson K
  TITLE     Probing coenzyme A homeostasis with semisynthetic biosensors.
  JOURNAL   Nat Chem Biol. 2022 Oct 31. doi: 10.1038/s41589-022-01172-7.
  PUBMED    36316571
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Oct 31. doi: 10.1038/s41589-022-01172-7."
COMMENT     Sequence Label: pCDNA5/FRT_COX8-CoA-Snifit(G41S)
FEATURES             Location/Qualifiers
     source          1..8048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        288..667
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        668..871
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     822..842
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     916..935
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        916..934
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             982..1056
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1069..1140
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1159..1230
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1243..1314
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1345..2229
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             join(2335..2337,2338..2850)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVE"
     primer_bind     complement(2377..2398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2985..3006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4053..4070)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4059..4283
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4416..4435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4567..4614
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4622..5644
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4622..5644
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5774..5895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5811..5830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5865..5884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5944..5960)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5944..5960)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5957..5979)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5968..5984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5992..5998,5999..6016,6017..6022))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6037..6058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6175..6192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6346..6934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6426..6445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7105..7896,7897..7965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7728..7747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7966..8048,1..22))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
       61 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      121 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      181 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      241 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      301 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      361 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      481 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      541 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      601 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      661 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      721 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      781 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      841 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      901 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
      961 cttaagcttg gtaccgccac catgtctgtg ctgacacctc tgctgctgag aggcctgaca
     1021 ggctctgcta gaaggctgcc tgtgcctaga gccaagatcc actctctgag cgtgctgacc
     1081 ccactgctcc ttaggggact gactggatct gctcggagac tgccagtgcc tcgggccaaa
     1141 attcatagcc tcggcggatc cgtcctgact ccactccttc tgagaggact caccggcagt
     1201 gctagacgcc tgccagttcc aagggctaag atccacagtc tgtcagtgct cactcccctg
     1261 ttgctcagag ggctgactgg aagtgcaaga cgactccccg tgccacgcgc caagattcat
     1321 tctcttggag gctctggcgg cagcgagatc ggaaccggct ttcctttcga ccctcactac
     1381 gtggaagtgc tgggcgagag aatgcactat gtggacgtgg gccccagaga tggaacccct
     1441 gtgctgtttc tgcacggcaa ccctaccagc agctacgtgt ggcggaacat catccctcac
     1501 gtggccccta cacacagatg tatcgcccct gacctgatcg gcatgggcaa gagcgataag
     1561 cccgacctgg gctacttctt cgacgaccac gtgcggttca tggacgcctt tattgaggcc
     1621 ctcggcctgg aagaggtggt gctggttatt cacgattggg gcagcgccct gggattccac
     1681 tgggccaaga gaaaccccga gagagtgaag ggaatcgcct tcatggaatt catcaggccc
     1741 attcctacct gggacgagtg gcctgagttc gccagagaga cattccaggc cttcagaacc
     1801 accgacgtgg gcagaaagct gatcatcgac cagaacgtgt tcatcgaggg caccctgcct
     1861 atgggagtcg ttagacctct gaccgaggtg gaaatggacc actacagaga gccctttctg
     1921 aaccccgtgg accgggaacc tctttggaga ttccccaacg agctgcccat tgccggcgag
     1981 cctgctaata ttgtggccct ggtggaagag tacatggact ggctgcatca gagccccgtg
     2041 cctaagctgc tgttttgggg aacacccggc gtgctgattc ctcctgctga agctgccaga
     2101 ctggccaaga gcctgcctaa ttgcaaggcc gtggatatcg gccctggcct gaatctgctg
     2161 caagaggaca accccgatct gatcggctcc gagattgcca gatggctgag caccctggaa
     2221 atcagcggag gatctctggc tgaagccgcc gctaaagaag ccgctgcaaa agaggctgcc
     2281 gccaaagagg cagcagcaaa agaagcagcc gctaaggctg ctgctggcgg atctgtgagc
     2341 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2401 aacggccaca agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg
     2461 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2521 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcgccacgac
     2581 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatcag cttcaaggac
     2641 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2701 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2761 tacaacttca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     2821 gccaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac
     2881 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2941 acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3001 ttcgtgaccg ccgccgggat cactcacggc ggatcccctc accacgtgtt cgtgtccgac
     3061 ttcgtggact tcagcatcta cgtggacgcc cctgaggatc tgctgcagac ctggtacatc
     3121 aaccggtttc tgaagttcag agagggcgcc tacacagacc ccgacagcta cttccacaac
     3181 tacgccaagc tgaccaaaga ggaagccatc aagaccgcca tgacctggtg gaaagagatc
     3241 aactggctga acctgaagca gaacatcctg cctaccagag agcgggccag cctgatcctg
     3301 accaaatctg ccaatcacgc cgtggaagaa gtgcggctga gaaaaggatc tggcggcacc
     3361 ggcacaccct acctgcagtt cgatagaaac cagtgggccg ctctgagaga cagcgtgcca
     3421 atgacactga gcgaggacga gatcgccaga ctgaagagca tcaatgagga cctgagcctg
     3481 gaagaggtgg ccgagattta cctgcccctg agccggctgc tgaacttcta catcagcagc
     3541 aacctgcgga gacaggccgt gctggaacag ttcctgggaa caaacggcca gcggatcccc
     3601 tacatcatct ctatcgccgg atctgtggcc gtgggcaaga gcacaacagc tagagttctg
     3661 caggccctgc tgagcagatg gcccgagcat agaagagtgg aactgattac caccgccggc
     3721 tttctgcacc ccaaccaggt gctgaaagaa cggggcctga tgaagaagaa gggcttcccc
     3781 gagagctacg acatgcaccg gctggtcaag tttgtgtccg acctgaagtc cggcgtgccc
     3841 aatgtgacag cccctgtgta cagccacctg atctacgacg tgatccccga cggcgataag
     3901 accgtggtgc agcccgatat cctgattctg gaaggcctga acgtgctgca gagcggcatg
     3961 gactatcctc atgatggcgc tccaggattc tcctcaattt ccgcttaagc ggccgctcga
     4021 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4081 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4141 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4201 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4261 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4321 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4381 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4441 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4501 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4561 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     4621 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     4681 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     4741 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     4801 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     4861 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     4921 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     4981 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5041 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5101 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5161 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5221 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5281 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     5341 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     5401 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5461 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5521 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     5581 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     5641 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5701 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5761 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5821 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5881 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6001 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6061 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7081 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7141 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7201 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7261 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7321 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7381 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7441 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7501 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7561 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7621 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7681 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7741 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7801 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7861 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7921 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8041 ttagaaaa
//
