LOCUS       sequence-38742-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Bakkenist CJ, Kastan MB
  TITLE     DNA damage activates ATM through intermolecular autophosphorylation 
            and dimer dissociation.
  JOURNAL   Nature. 2003 Jan 30;421(6922):499-506.
  PUBMED    12556884
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2003-01-30"; volume: "421(6922)"; pages: "499-506"
COMMENT     Sequence Label: hATMS1981A
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             192..209
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga
      121 attctgcaga tatccatcac actggcggcc gctcgaccac catggactac aaggacgatg
      181 acgacaagat gcatcatcat catcatcata tgagtctagt acttaatgat ctgcttatct
      241 gctgccgtca actagaacat gatagagcta cagaacgaaa gaaagaagtt gagaaattta
      301 agcgcctgat tcgagatcct gaaacaatta aacatctaga tcggcattca gattccaaac
      361 aaggaaaata tttgaattgg gatgctgttt ttagattttt acagaaatat attcagaaag
      421 aaacagaatg tctgagaata gcaaaaccaa atgtatcagc ctcaacacaa gcctccaggc
      481 agaaaaagat gcaggaaatc agtagtttgg tcaaatactt catcaaatgt gcaaacagaa
      541 gagcacctag gctaaaatgt caagaactct taaattatat catggataca gtgaaagatt
      601 catctaatgg tgctatttac ggagctgatt gtagcaacat actactcaaa gacattcttt
      661 ctgtgagaaa atactggtgt gaaatatctc agcaacagtg gttagaattg ttctctgtgt
      721 acttcaggct ctatctgaaa
//
