LOCUS       sequence-387443-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for the expression of 4X human ubiquitin in tandem (SMC interal
            no. 538).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, 
            Martens S
  TITLE     Oligomerization of p62 allows for selection of ubiquitinated cargo 
            and isolation membrane during selective autophagy.
  JOURNAL   Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.
  PUBMED    26413874
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-09-28"; volume: "4:e08941. doi"; pages: " 
            10.7554/eLife.08941"
COMMENT     Sequence Label: pGEX-4T1_4xUb
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..51
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             108..335
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             336..563
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             564..791
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
       61 atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcatatg cagatcttcg
      121 tgaagaccct gaccggcaag accatcaccc tagaggtgga gcccagtgac accatcgaga
      181 acgtgaaggc caagatccag gataaagagg gcatcccccc tgaccagcag aggctgatct
      241 ttgccggcaa gcagctggaa gatggccgca ccctctctga ttacaacatc cagaaggagt
      301 caaccctgca cctggtcctt cgcctgagag gtggcatgca gatcttcgtg aagaccctga
      361 ccggcaagac catcaccctg gaggtggagc ccagtgacac catcgagaac gtgaaggcca
      421 agatccagga taaagagggc atcccccctg accagcagag gctgatcttt gccggcaagc
      481 agctggaaga tggccgcacc ctctctgatt acaacatcca gaaagagtca accctgcacc
      541 tggtcctccg tctgaggggt ggcatgcaga tcttcgtgaa gaccctgact ggcaagacca
      601 tcaccctgga ggtggagccc agtgacacca tcgagaacgt gaaggccaag atccaggata
      661 aagagggcat cccccctgac cagcagaggc tgatctttgc cggcaagcag ctggaagatg
      721 gccgcaccct ctctgattac aacatccaga aggagtcaac cctgcacctg gtccttcgcc
      781 tgagaggtgg catgcagatc ttcgtgaaga ccctgaccgg caagaccatc accttggagg
      841 tggagcccag tgacaccatc gagaatgtga aggccaagat ccaggataaa gagggcatcc
      901 cccctgacca gcagaggctg atctttgccg gcaagcagct agaagatggc cgcactctct
      961 ctgattacaa catccagaaa ga
//
