LOCUS       sequence-387446-        5380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express VSV-G protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Jneid B, Bochnakian A, Hoffmann C, Delisle F, Djacoto E, Sirven P, 
            Denizeau J, Sedlik C, Gerber-Ferder Y, Fiore F, Akyol R, Brousse C, 
            Kramer R, Walters I, Carlioz S, Salmon H, Malissen B, Dalod M, 
            Piaggio E, Manel N
  TITLE     Selective STING stimulation in dendritic cells primes antitumor T 
            cell responses.
  JOURNAL   Sci Immunol. 2023 Jan 13;8(79):eabn6612. doi: 
            10.1126/sciimmunol.abn6612. Epub 2023 Jan 13.
  PUBMED    36638189
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-13"; volume: "8(79):eabn6612. doi"; pages:
            " 10.1126/sciimmunol.abn6612. Epub 2023 Jan 13"
COMMENT     Sequence Label: pVAX1-VSVG-INDIANA2
FEATURES             Location/Qualifiers
     source          1..5380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        37..416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        417..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     571..591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     617..641
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          732..1304
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             join(1390..1437,1438..2925)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(3206..3223)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3212..3436
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             3609..4403
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3609..4403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3663..3682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4273..4292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      4728..5316
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5217..5236
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      601 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      661 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      721 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      781 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      841 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      901 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
      961 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1021 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1081 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1141 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1201 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1261 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1321 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc ctcgaggaat
     1381 tctgacacta tgaagtgcct tttgtactta gcctttttat tcattggggt gaattgcaag
     1441 ttcaccatag tttttccaca caaccaaaaa ggaaactgga aaaatgttcc ttctaattac
     1501 cattattgcc cgtcaagctc agatttaaat tggcataatg acttaatagg cacagcctta
     1561 caagtcaaaa tgcccaagag tcacaaggct attcaagcag acggttggat gtgtcatgct
     1621 tccaaatggg tcactacttg tgatttccgc tggtatggac cgaagtatat aacacattcc
     1681 atccgatcct tcactccatc tgtagaacaa tgcaaggaaa gcattgaaca aacgaaacaa
     1741 ggaacttggc tgaatccagg cttccctcct caaagttgtg gatatgcaac tgtgacggat
     1801 gccgaagcag tgattgtcca ggtgactcct caccatgtgc tggttgatga atacacagga
     1861 gaatgggttg attcacagtt catcaacgga aaatgcagca attacatatg ccccactgtc
     1921 cataactcta caacctggca ttctgactat aaggtcaaag ggctatgtga ttctaacctc
     1981 atttccatgg acatcacctt cttctcagag gacggagagc tatcatccct gggaaaggag
     2041 ggcacagggt tcagaagtaa ctactttgct tatgaaactg gaggcaaggc ctgcaaaatg
     2101 caatactgca agcattgggg agtcagactc ccatcaggtg tctggttcga gatggctgat
     2161 aaggatctct ttgctgcagc cagattccct gaatgcccag aagggtcaag tatctctgct
     2221 ccatctcaga cctcagtgga tgtaagtcta attcaggacg ttgagaggat cttggattat
     2281 tccctctgcc aagaaacctg gagcaaaatc agagcgggtc ttccaatctc tccagtggat
     2341 ctcagctatc ttgctcctaa aaacccagga accggtcctg ctttcaccat aatcaatggt
     2401 accctaaaat actttgagac cagatacatc agagtcgata ttgctgctcc aatcctctca
     2461 agaatggtcg gaatgatcag tggaactacc acagaaaggg aactgtggga tgactgggca
     2521 ccatatgaag acgtggaaat tggacccaat ggagttctga ggaccagttc aggatataag
     2581 tttcctttat acatgattgg acatggtatg ttggactccg atcttcatct tagctcaaag
     2641 gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt cgcaacttcc tgatgatgag
     2701 agtttatttt ttggtgatac tgggctatcc aaaaatccaa tcgagcttgt agaaggttgg
     2761 ttcagtagtt ggaaaagctc tattgcctct tttttcttta tcatagggtt aatcattgga
     2821 ctattcttgg ttctccgagt tggtatccat ctttgcatta aattaaagca caccaagaaa
     2881 agacagattt atacagacat agagatgaac cgacttggaa agtaactcaa atcctgcaca
     2941 acagattctt catgtttgga ccaaatcaac ttgtgatacc atgctcaaag aggcctcaat
     3001 tatatttgag tttttaattt ttatgaaaaa aaaaaaaaaa aacggaattc ctcgagggat
     3061 ccgtcgagga attcactcgc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
     3121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
     3181 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3241 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3301 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3361 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3421 gcggtgggct ctatggcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc
     3481 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     3541 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     3601 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3661 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3721 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3781 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3841 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3901 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3961 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4021 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4081 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4141 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4201 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4261 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4321 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4381 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
     4441 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
     4501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4561 caataaccct gataaatgct tcaataatag cacgtgctaa aacttcattt ttaatttaaa
     4621 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4681 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4741 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4861 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4921 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4981 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5041 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5101 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5161 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5221 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5281 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5341 cggttcctgg ccttttgctg gccttttgct cacatgttct
//
