LOCUS       sequence-387447-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human synapsin promoter driven dTomato expression at human AAVS1 
            safe harbour locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, 
            Durnberger G, Mechtler K, Knoblich JA
  TITLE     Integrated transcriptome and proteome analysis reveals 
            posttranscriptional regulation of ribosomal genes in human brain 
            organoids.
  JOURNAL   Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.
  PUBMED    36989136
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-29"; volume: "12:e85135. doi"; pages: " 
            10.7554/eLife.85135"
COMMENT     Sequence Label: AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      375..963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     864..883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1117..1134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1251..1272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1287..1292,1293..1310,1311..1317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1325..1341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1330..1352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1349..1365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1349..1365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1386..1404
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1439..2242
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    2249..2274
                     /label=SA
                     /note="splice acceptor site"
     CDS             2298..2351
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2361..2960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2361..2380)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2857..2877
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2992..3009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2998..3222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3231..3249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3880..4327
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          4366..4498
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4481..4500
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      4534..4543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4540..5241
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(4673..4691)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4941..4960
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5135..5158
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5251..5839
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5304..5324)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5722..5733)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5994..6115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6031..6050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6085..6104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    6789..7625
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(7660..7678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7685..7702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7685..7701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7694..7716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7839..8141
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8020..8039
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8490..9284
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8490..9284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8544..8563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9154..9173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9534..10143,1..182,183..251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1141 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1201 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1261 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1321 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     1381 ctcagaatta accctcacta aagggactag tcctgcaggt ttaaacgaat tcgccctttg
     1441 ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg
     1501 cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct
     1561 tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg
     1621 gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc
     1681 tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg
     1741 gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc
     1801 tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt
     1861 ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt
     1921 gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca
     1981 tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct
     2041 gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc
     2101 ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg
     2161 gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt
     2221 cccctccacc ccacagtggg gcaagcttct gacctcttct cttcctccca cagggcctcg
     2281 agagatctgg cagcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     2341 atcccggccc taggctcgag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     2401 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     2461 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     2521 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     2581 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     2641 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     2701 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     2761 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     2821 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     2881 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     2941 cccgcaagcc cggtgcctga tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     3001 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3061 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3121 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3181 aagacaatag caggcatgct ggggatgcgg tgggctctat gggtaccggt taatacgact
     3241 cactataggg aacaaaagct gggtaccggg ccccccctcg agggagctca cggggagagc
     3301 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     3361 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     3421 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     3481 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag
     3541 agatttagaa tgacagggta ccggagctca cggggagagc ccccccccaa agcccccagg
     3601 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     3661 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     3721 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     3781 acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa tgacagtcta
     3841 gagcggatgc atatcgatct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac
     3901 caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca
     3961 cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat
     4021 gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc
     4081 tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc
     4141 cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg
     4201 accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc
     4261 cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag
     4321 agcgcagcct taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
     4381 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     4441 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     4501 gtccactccc agttcaatta cagcggcgcg cccgccacca tggtgagcaa gggcgaggag
     4561 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     4621 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4681 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     4741 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     4801 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     4861 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     4921 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4981 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     5041 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     5101 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     5161 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac
     5221 ggcatggacg agctgtacaa gtaattaatt aatcaacctc tggattacaa aatttgtgaa
     5281 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5341 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5401 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5461 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5521 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5581 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5641 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5701 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5761 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5821 ctttgggccg cctccccgca taagtcgacg gtaccgcggg cccgggatcc accggatcta
     5881 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5941 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     6121 taaattgtaa gcgttaatat tttgttaaaa ttcgccgaat tgggtaccgg gccccccctc
     6181 gagggagctc acggggagag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     6241 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     6301 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     6361 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     6421 aaaagcttta ggctgaaaga gagatttaga atgacagggt accggagctc acggggagag
     6481 ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg
     6541 agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg
     6601 cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg
     6661 ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgaaaga
     6721 gagatttaga atgacagtct agagcggccg ccaccgcggt ggagctccag ccgacagtac
     6781 taagctttac tagggacagg attggtgaca gaaaagcccc atccttaggc ctcctccttc
     6841 ctagtctcct gatattgggt ctaaccccca cctcctgtta ggcagattcc ttatctggtg
     6901 acacaccccc atttcctgga gccatctctc tccttgccag aacctctaag gtttgcttac
     6961 gatggagcca gagaggatcc tgggagggag agcttggcag ggggtgggag ggaagggggg
     7021 gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct accctctccc agaacctgag
     7081 ctgctctgac gcggctgtct ggtgcgtttc actgatcctg gtgctgcagc ttccttacac
     7141 ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa taagttggtc ctgagttcta
     7201 actttggctc ttcacctttc tagtccccaa tttatattgt tcctccgtgc gtcagtttta
     7261 cctgtgagat aaggccagta gccagccccg tcctggcagg gctgtggtga ggaggggggt
     7321 gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg
     7381 ccgcctctac tccctttctc tttctccatc cttctttcct taaagagtcc ccagtgctat
     7441 ctgggacata ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct gctctgggct
     7501 tctgggtttg agtccttggc aagcccagga gaggcgctca ggcttccctg tcccccttcc
     7561 tcgtccacca tctcatgccc ctggctctcc tgccccttcc ctacaggggt tcctggctct
     7621 gctctaaggg cgaattcgcg gccgctaaat tcaattcgcc ctatagtgag tcgtattaca
     7681 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7741 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7801 atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt tacacctata
     7861 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgccgg
     7921 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     7981 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     8041 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     8101 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc atgagattat
     8161 caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct
     8221 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     8281 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     8341 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     8401 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagctctg
     8461 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     8521 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     8581 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8641 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     8701 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8761 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8821 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8881 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8941 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     9001 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     9061 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     9121 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     9181 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     9241 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt aacgcttaca
     9301 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt
     9361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9421 aatatgtatc cgctcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10081 ggtgaatact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10141 ccg
//
