LOCUS       sequence-387449-       11269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reverse complement of let7-miRNA binding sites (3x) were integrated 
            in between the stop codon and MS2 stem-loops in the SunTag 
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: Let7R-SunTag
FEATURES             Location/Qualifiers
     source          1..11269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4085..4109
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4186..4318
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4301..4320
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4363..4381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4385..4394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4460..4516
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4532..4588
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4604..4660
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4676..4732
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4748..4804
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4820..4876
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4892..4948
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4964..5020
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5036..5092
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5108..5164
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5180..5236
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5252..5308
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5324..5380
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5396..5452
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5468..5524
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5540..5596
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5612..5668
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5684..5740
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5756..5812
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5828..5884
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5900..5956
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5972..6028
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6116..6172
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6206..7135
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7136..7138,7139..7459)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7139..7462
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    9067..9201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9145..9164
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9309..9342)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9395..9411)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9427..9449)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9860..10217
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10282..10751
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     4081 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     4141 ataccaactt tccgtaccac ttcctaccct cgtaaaaccg gtcaggtaag tatcaaggtt
     4201 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     4261 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     4321 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
     4381 gctagccacc atgggcgcca tcatacacgc ggccgcaatc atcggtccgg gtggatctgg
     4441 aggtggaggt tctggaggag aagaactttt gagcaagaat tatcatcttg agaacgaagt
     4501 ggctcgtctt aagaaaggtt ctggcagtgg agaagaactg ctttcaaaga attaccacct
     4561 ggaaaatgag gtagctagac tgaaaaaggg gagcggaagt ggggaggagt tgctgagcaa
     4621 aaattatcat ttggagaacg aagtagcacg actaaagaaa gggtccggat cgggtgagga
     4681 gttactctcg aaaaattatc atctcgaaaa cgaagtggct cggctaaaaa agggcagtgg
     4741 ttctggagaa gagctattat ctaaaaacta ccacctcgaa aatgaggtgg cacgcttaaa
     4801 aaagggaagt ggcagtggtg aagagctact atccaagaat tatcatcttg agaacgaggt
     4861 agcgcgtttg aagaagggtt ccggctcagg agaggaactg ctctcgaaga actatcatct
     4921 tgaaaatgag gtcgctcgat taaaaaaggg atcgggcagt ggtgaggaac tactttcaaa
     4981 gaattaccac ctcgaaaacg aagtagctcg attaaagaaa ggttcagggt cgggtgaaga
     5041 attactgagt aaaaattatc atctggaaaa tgaggtagcg agactaaaaa aggggagtgg
     5101 ttctggcgaa gagttgctat cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa
     5161 aaagggctca ggctcaggcg aggagttgct ctcgaaaaac taccacttgg aaaatgaggt
     5221 cgcgaggttg aaaaagggga gcgggtcggg cgaggagtta ttgagcaaaa actatcattt
     5281 agagaacgaa gtcgcgcgct taaagaaagg ctcgggctcg ggcgaagaac tcttatcgaa
     5341 gaactaccac ctcgaaaatg aggtcgccag gttgaaaaag ggcagtggca gcggggagga
     5401 actcttgagc aagaactacc acttggagaa tgaggtcgcg agattgaaga aagggtcggg
     5461 gagcggcgag gaattgctca gcaagaatta tcatttggag aacgaagtcg ccaggctcaa
     5521 gaaaggctcg gggtcggggg aggaattgtt gagtaaaaac taccacttgg aaaatgaagt
     5581 cgccaggctc aaaaaaggga gtgggagcgg cgaagagtta ttgagcaaaa attaccactt
     5641 ggagaacgaa gtggcaaggc tcaagaaagg gagcggcagc ggggaggagc tcttatcgaa
     5701 gaactaccac ttagagaatg aagtcgcccg cttgaagaaa ggctcgggga gcggggaaga
     5761 gctcttgagc aagaactacc acttggaaaa tgaggtggcg cgcttgaaga aagggagcgg
     5821 gagcggggaa gagttactat ctaagaatta tcatctcgag aacgaggtgg ctcgactaaa
     5881 gaagggctcc ggcagtgggg aggaactcct gtcgaagaac tatcatcttg aaaatgaggt
     5941 tgcaagactt aaaaaggggt ccggatcagg tgaggaacta ctcagtaaga attaccacct
     6001 ggaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtgaagaac tgctctcaaa
     6061 agattatcat ttggaaaatg aggttgcacg tttaaaaaag ggaagtggca gtggtgagga
     6121 acttctgtcg aaaaattatc atctcgagaa tgaagtagcc cgacttaaaa agggttctgg
     6181 ctcgggtcag cggccgcacc ggtcagcttc caaggtgtac gaccccgagc aacgcaaacg
     6241 catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt
     6301 catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa
     6361 cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg
     6421 catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg
     6481 cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa
     6541 aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca
     6601 ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg
     6661 ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa
     6721 aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa
     6781 actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag
     6841 acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt
     6901 cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat
     6961 gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt
     7021 ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga
     7081 tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagatggg
     7141 agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac
     7201 ctgtgtggtg cactacaccg ggatgcttga agatggaaag aaagtcgatt cctcccggga
     7261 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga
     7321 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc
     7381 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt
     7441 ggagcttcta aaaccggaat aagggccagg gaagaactga tcgccgtgta accgcggtga
     7501 ggtagttcaa taggctgtgc aggcctgagg tagttcaata ggctgtgctc cgagtgaggt
     7561 agttcaatag gctgtccccg cggcgcgcgc gggatccgga tccaacctac aaacgggtgg
     7621 aggatcaccc cacccgacac ttcacaatca aggggtacaa tacacaaggg tggaggaaca
     7681 ccccaccctc cagacacatt acacagaaat ccaatcaaac agaagcacca tcagggcttc
     7741 tgctaccaaa tttatctcaa aaaactacaa caaggaatca ccatcaggga ttccctgtgc
     7801 aatatacgtc aaacgagggc cacgacggga ggacgatcac gcctcccgaa tatcggcatg
     7861 tctggctttc gaattcagtg cgtggagcat cagcccacgc agccaatcag agtcgaatac
     7921 aagtcgactt tcgcgaagag catcagcctt cgcgccattc ttacacaaac cacactctcc
     7981 cctacaggaa cagcatcagc gttcctgccc agtacccaac tcaagaaaat ttatgtcccc
     8041 atgcagcatc agcgcatggg ccccaagaat acatccccaa caaaatcaca tccgagcacc
     8101 aacagggctc ggagtgttgt ttcttgtcca actggacaaa ccctccatgg accatcaggc
     8161 catggactct caccaacaag acaaaaacta ctcttctcga agcagcatca gcgcttcgaa
     8221 acactcgagc atacattgtg cctatttctt gggtggacga tcacgccacc catgctctca
     8281 cgaatttcaa aacacggaca aggacgagca ccaccagggc tcgtcgttcc acgtccaata
     8341 cgattactta cctttcggga tcacgatcac ggatcccgca gctacatcac ttccactcag
     8401 gacattcaag catgcacgat cacggcatgc tccacaagtc tcaaccacag aaactaccaa
     8461 atgggttcag caccagcgaa cccactccta cctcaaacct cttcccacaa aactggcaag
     8521 caggatcacc gcttgcccat tccaacatac caaatcaaaa acaattactg gtacagcatc
     8581 agcgtaccag cccacatctc tcactactat caaaaaccaa accgttcagc aacagcgaac
     8641 ggtacacacg gaaaaatcaa ctggtttaca aatacgaaag acgatcacgc tttcgtccag
     8701 cgcaaactat tacgaaaaac atccgacggg aagagcaaca gccttcccgc ggcggaaaac
     8761 ctcacaaaaa cacgacaaac ggatgcacga acacggcatc cgccgacaac ccacaaactt
     8821 acaaccaggc aaacggtgca ggatcaccgc accgtacatc aaacacctca gatctcattt
     8881 cgcgcgggat ccagaccacc tcccctgcga gctaagctgg acagccaatg acgggtaaga
     8941 gagtgacatt tttcactaac ctaagacagg agggccgtca gagctactgc ctaatccaaa
     9001 gacgggtaaa agtgataaaa atgtatcact ccaacctaag acaggcgcag cttccgaggg
     9061 atttgagatc cagacatgat aagatacatt gatgagtttg gacaaaccaa aactagaatg
     9121 cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta ttgccttatt tgtaaccatt
     9181 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     9241 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     9301 tatgatctga agttcctata ctatttgaag aataggaact tcggaatagg aacttcggat
     9361 ctcatatggc catatggggg cgcctagaga aggagtgagg gctggataaa gggaggatcg
     9421 aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg
     9481 cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc
     9541 ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg aaaagacctt
     9601 gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt
     9661 aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga catctaccga
     9721 ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac ttaagggagt
     9781 ggtaacaggc tagcccgggc ccatattttc agacaaatac agaaacacag tcagacagag
     9841 acaacacaga acgatgctgc agcagacaag acgcgcggcg cggcttcggt cccaaaccga
     9901 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
     9961 atatccctcc tctaaggggg gtgcaccaaa gagtcctaaa cgatcgggat tttaggactc
    10021 aggtcgggcc acaaaaacgg cccccgaagt ccctgggacg tctcccaggg ttgcggccgg
    10081 gtgttccgaa ctcgtcagtt ccaccacggg tccgccagat acagagctag ttagctaact
    10141 agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac agacacagat
    10201 aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca ggggtctccc
    10261 gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat cactcagagg
    10321 agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
    10381 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgcgccgag tgaggggttg
    10441 tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg
    10501 attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat
    10561 ctgatggttc tctagaaact gctgagggct ggaccgcatc tggggaccat ctgttcttgg
    10621 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
    10681 tccatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
    10741 cccatattca gctgttccat ctgttcctga ccttgatctg aacttttcta ttctcagtta
    10801 tgtatttttc catgccttgc aaaatggcgt tacttaagct agcttgccaa acctacaggt
    10861 ggggtctttc acatgtatat gtcaaaaata aaaatcaact aattgactag taattaatat
    10921 gactggcata atgggaaatt gatcctgaca gatgcaaact ggcttctcag cagcgcattt
    10981 atgttgtcaa ctgaggaagg aaacgttaat gacagaaact ctaagtaatt tccacgttta
    11041 tctattttta tttatactag ctttggtaac aggaatattg cagcattcat gcacattgaa
    11101 acccttatga aataaaaaca tctgtgcatt taaaatggaa ttaacatttt aaatgttaaa
    11161 aaaagctggc ttagcttccc cccgccccct agggcataga acaagtcaaa tgctttatat
    11221 atttgagttt gggatgtatt aggaaactcc taagagcaaa gctagcttg
//
