LOCUS       sequence-387457-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Let7-miRNA binding sites (3x) were integrated in between the stop 
            codon and TREAT cassette in IRE-TREAT reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: Let7-IRE-TREAT
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4086..4110
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4330..5265
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8815..8949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8893..8912
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9057..9090)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9143..9159)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9175..9197)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9608..9965
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10030..10499
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataaag ctttaggcgt gtacggtggg cgcctataaa
     4081 agcagagctc gtttagtgaa ccgtcagatc gcctgcttcc agacgttctt cgccgagagt
     4141 cgtcggggtt tcctgcttca acagtgcttg gacggaaccc ggcgctcgtt ccccaccccg
     4201 gccggccgcc catagccagc cctccgtcac ctcttcaccg caccctcgga ctgccccaag
     4261 gcccccgccg ccgctccagc gccgcgcagc caccgccgcc gccgccgcct ctccttagtc
     4321 gccgccatga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     4381 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     4441 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     4501 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     4561 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     4621 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     4681 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     4741 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     4801 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     4861 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     4921 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     4981 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     5041 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     5101 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     5161 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     5221 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaagggcc agggaagaac
     5281 tgatcgccgt gtaaccgcgg ggacagccta ttgaactacc tcactcggag cacagcctat
     5341 tgaactacct caggcctgca cagcctattg aactacctca ccgcggcgcg cgcgggatcc
     5401 ggatccaacc tacaaaagat cctacctgca aagcaccaga ggatatggcc tcggtgcctc
     5461 agcatcttca gatgtgctgt cactgtccaa tcttcaagcc agcagagcat atgggctcgc
     5521 tggctccact gtcattacag tggacctgat cttctccaat caaaggagca gaggatatgg
     5581 cctcgctccc tccgactaca tcaggctgga agtgacatcc atcattcatc ccagcagagc
     5641 atatgggctc gctgggacca cagcatgcct cgaagtgctg agagactatc atcaaaccga
     5701 cagaggatat ggcctcgtcg gctcctactc cctcctggtg gaatacttct ccactcttca
     5761 acccagcaga gcatatgggc tcgctgggtc catcagagat acaagatctc tcgacagatc
     5821 ctacctgcaa agcaccagag gatatggcct cggtgcctca gcatcttcag atgtgctgtc
     5881 actgtccaat cttcaagcca gcagagcata tgggctcgct ggctccactg tcattacagt
     5941 ggacctgatc ttctccaatc aaaggagcag aggatatggc ctcgctccct ccgactacat
     6001 caggctggaa gtgacatcca tcattcatcc cagcagagca tatgggctcg ctgggaccac
     6061 agcatgcctc gaagtgctga gagactatca tcaaaccgac agaggatatg gcctcgtcgg
     6121 ctcctactcc ctcctggtgg aatacttctc cactcttcaa cccagcagag catatgggct
     6181 cgctgggtcc atcagagata caagatctct cgacagatcc tacctgcaaa gcaccagagg
     6241 atatggcctc ggtgcctcag catcttcaga tgtgctgtca ctgtccaatc ttcaagccag
     6301 cagagcatat gggctcgctg gctccactgt cattacagtg gacctgatct tctccaatca
     6361 aaggagcaga ggatatggcc tcgctccctc cgactacatc aggctggaag tgacatccat
     6421 cattcatccc agcagagcat atgggctcgc tgggaccaca gcatgcctcg aagtgctgag
     6481 agactatcat caaaccgaca gaggatatgg cctcgtcggc tcctactccc tcctggtgga
     6541 atacttctcc actcttcaac ccagcagagc atatgggctc gctgggtcca tcagagatac
     6601 aagatctctc gacagatcct acctgcaaag caccagagga tatggcctcg gtgcctcagc
     6661 atcttcagat gtgctgtcac tgtccaatct tcaagccagc agagcatatg ggctcgctgg
     6721 ctccactgtc attacagtgg acctgatctt ctccaatcaa aggagcagag gatatggcct
     6781 cgctccctcc gactacatca ggctggaagt gacatccatc attcatccca gcagagcata
     6841 tgggctcgct gggaccacag catgcctcga agtgctgaga gactatcatc aaaccgacag
     6901 aggatatggc ctcgtcggct cctactccct cctggtggaa tacttctcca ctcttcaacc
     6961 cagcagagca tatgggctcg ctgggtccat cagagataca agatctctcg agccgcggtg
     7021 cgtaagtctg ttaacagaat gctgcatacg ctgagtactt cgaaatgtca tcctctgtct
     7081 gacactgaac gtaatccaga cgcgtaagtc aggccggaaa attcccgcca ccggaagttg
     7141 agtagacggt gctgcctgcg actcaacccc aggaggactg ggtgaacaaa gctgcgaagt
     7201 gatccatgta agccctcaga accgtctcgg aaagaggacc ccacatgttg tagcttcaag
     7261 gcccaatgtc agaccacgcc atggcgtgcc actctgcgga gagtgcagtc tgcgacagtg
     7321 ccccaggagg actgggtgag gatcctacct acaaacggca cgagcatcag ccgtgccgcc
     7381 cagcatcttc atcactgtcc aatcttcaag cgtgcacgat cacggcacgc tccactgtca
     7441 ttacagtctt atccaatcaa actccagcag catcagcgct ggagctccga ctacatcagg
     7501 acatccatca ttcaacgctc gacgatcacg cgagcgtcca cagcatgcct cagacactat
     7561 catcaaacgg ctgcacgatc acggcagccg ctcctactgc ctctacgtta tcgactcttc
     7621 tgcgacgagc atcagccgtc gcaacatcgg acataccaga tcctacctac aaacggcacg
     7681 agcatcagcc gtgccgccca gcatcttcat cactgtccaa tcttcaagcg tgcacgatca
     7741 cggcacgctc cactgtcatt acagtcttat ccaatcaaac tccagcagca tcagcgctgg
     7801 agctccgact acatcaggac atccatcatt caacgctcga cgatcacgcg agcgtccaca
     7861 gcatgcctca gacactatca tcaaacggct gcacgatcac ggcagccgct cctactgcct
     7921 ctacgttatc gactcttctg cgacgagcat cagccgtcgc aacatcggac ataccagatc
     7981 ctacctacaa acggcacgag catcagccgt gccgcccagc atcttcatca ctgtccaatc
     8041 ttcaagcgtg cacgatcacg gcacgctcca ctgtcattac agtcttatcc aatcaaactc
     8101 cagcagcatc agcgctggag ctccgactac atcaggacat ccatcattca acgctcgacg
     8161 atcacgcgag cgtccacagc atgcctcaga cactatcatc aaacggctgc acgatcacgg
     8221 cagccgctcc tactgcctct acgttatcga ctcttctgcg acgagcatca gccgtcgcaa
     8281 catcggacat accagatcct acctacaaac ggcacgagca tcagccgtgc cgcccagcat
     8341 cttcatcact gtccaatctt caagcgtgca cgatcacggc acgctccact gtcattacag
     8401 tcttatccaa tcaaactcca gcagcatcag cgctggagct ccgactacat caggacatcc
     8461 atcattcaac gctcgacgat cacgcgagcg tccacagcat gcctcagaca ctatcatcaa
     8521 acggctgcac gatcacggca gccgctccta ctgcctctac gttatcgact cttctgcgac
     8581 gagcatcagc cgtcgcaaca tcggacatac cagatcttca atcgatttcg cgcgggatcc
     8641 agaccacctc ccctgcgagc taagctggac agccaatgac gggtaagaga gtgacatttt
     8701 tcactaacct aagacaggag ggccgtcaga gctactgcct aatccaaaga cgggtaaaag
     8761 tgataaaaat gtatcactcc aacctaagac aggcgcagct tccgagggat ttgagatcca
     8821 gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa
     8881 tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat
     8941 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     9001 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctgaag
     9061 ttcctatact atttgaagaa taggaacttc ggaataggaa cttcggatct catatggcca
     9121 tatgggggcg cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga
     9181 acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg
     9241 gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat
     9301 gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg
     9361 ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca
     9421 ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc
     9481 gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacaggcta
     9541 gcccgggccc atattttcag acaaatacag aaacacagtc agacagagac aacacagaac
     9601 gatgctgcag cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa gcaaaaattc
     9661 agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat atccctcctc
     9721 taaggggggt gcaccaaaga gtcctaaacg atcgggattt taggactcag gtcgggccac
     9781 aaaaacggcc cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt gttccgaact
     9841 cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag taccgacgca
     9901 ggcgcataaa atcagtcata gacactagac aatcggacag acacagataa gttgctggcc
     9961 agcttacctc ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga
    10021 gcccccaaat gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa
    10081 ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac
    10141 ccgggcgact cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta
    10201 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
    10261 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
    10321 tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg
    10381 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct
    10441 tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc
    10501 tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg tatttttcca
    10561 tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcac
    10621 atgtatatgt caaaaataaa aatcaactaa ttgactagta attaatatga ctggcataat
    10681 gggaaattga tcctgacaga tgcaaactgg cttctcagca gcgcatttat gttgtcaact
    10741 gaggaaggaa acgttaatga cagaaactct aagtaatttc cacgtttatc tatttttatt
    10801 tatactagct ttggtaacag gaatattgca gcattcatgc acattgaaac ccttatgaaa
    10861 taaaaacatc tgtgcattta aaatggaatt aacattttaa atgttaaaaa aagctggctt
    10921 agcttccccc cgccccctag ggcatagaac aagtcaaatg ctttatatat ttgagtttgg
    10981 gatgtattag gaaactccta agagcaaagc tagcttg
//
