LOCUS       sequence-387470-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G 
            gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as 
            optimal components of a BRET2 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple GaoB
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3010..3027)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3039..3612
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3062..3614
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3206..3223)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3433..3452
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5753..5770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5759..5983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6029..6457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6116..6135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6326..6347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6466..6486)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6471..6800
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6651..6786
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6713..6732
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6867..7661
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6921..6940)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7531..7550
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7835..7956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7872..7891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7926..7945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8005..8021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8005..8021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8018..8040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8029..8045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8053..8059,8060..8077,8078..8083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8098..8119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8236..8253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8407..8995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8487..8506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9166..9957,9958..10026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9789..9808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10027..10131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtctaaggg agaggagctg ttcaccggag tggtgcctat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gagctacggc gtgcagtgtt tttcccggta
     2221 tcccgaccac atgaagcagc acgatttctt taagagcgcc atgcctgagg gctacgtgca
     2281 ggagaggaca atcttcttta aggacgatgg caactataag acccgcgccg aggtgaagtt
     2341 cgagggcgac acactggtga accggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt acatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 ctccgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gaccagtgct
     2581 gctgcccgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggaatcacac tgggaatgga
     2701 cgagctgtat aagggctccg ccggcaccat gtctaacaat atggccaaga tcgccgaggc
     2761 cagaaagaca gtggagcagc tgaagctgga ggtcaatatc gataggatga aggtgtctca
     2821 ggctgccgcc gagctgctgg ccttttgcga gacccacgcc aaggacgatc ctctggtgac
     2881 acccgtgcct gcagcagaga acccattccg ggataagcgg ctgttctgcg tgctgctgtg
     2941 accgcggcgt acgaggcctg catgctccgg acctgcaggt tcgaagtcga cagatctcaa
     3001 ttggggcccc tatagtgtca cctaaataat tccgcccccc cctctccctc ccccccccct
     3061 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3121 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3181 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3241 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3301 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3361 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3421 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3481 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3541 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3601 acacgatgat aatatgggct gcaccctgtc cgcagaggag agggcagcac tggagaggtc
     3661 taaggcaatc gagaagaacc tgaaggagga cggcatcagc gccgccaagg atgtgaagct
     3721 gctgctgctg ggagcaggag agtctggcaa gagcacaatc gtgaagcaga tgaagatcat
     3781 ccacgaggat ggcttctctg gcgaggacgt gaagcagtac aagcccgtgg tgtattccaa
     3841 taccatccag tctctggccg ccatcgtgag ggcaatggac acactgtccg gaggaggagg
     3901 ctccatggcc tctaaggtgt acgatccaga gcagaggaag cgcatgatca ccggaccaca
     3961 gtggtgggca aggtgcaagc agatgaacgt gctggactct tttatcaatt actatgatag
     4021 cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aatgccacaa gctcctacct
     4081 gtggaggcac gtggtgcccc acatcgagcc tgtggcaagg tgtatcatcc cagacctgat
     4141 cggcatgggc aagagcggca agtccggcaa cggctcctat cggctgctgg atcactacaa
     4201 gtatctgacc gcctggttcg agctgctgaa tctgcccaag aagatcatct ttgtgggaca
     4261 cgactgggga gcagcactgg cattccacta cgcctatgag caccaggaca gaatcaaggc
     4321 catcgtgcac atggagtccg tggtggatgt gatcgagtct tgggacgagt ggcctgacat
     4381 cgaggaggac atcgccctga tcaagtctga ggagggcgag aagatggtgc tggagaacaa
     4441 tttctttgtg gagaccgtgc tgccaagcaa gatcatgagg aagctggagc cagaggagtt
     4501 cgcagcatac ctggagcctt ttaaggagaa gggcgaggtg cggagaccta cactgagctg
     4561 gccacgcgag atcccactgg tgaagggagg caagccagac gtggtgcaga tcgtgcggaa
     4621 ctacaatgcc tatctgagag cctccgacga tctgcccaag ctgttcatcg agtccgatcc
     4681 tggcttcttt tctaacgcca tcgtggaggg cgccaagaag ttccccaata ccgagtttgt
     4741 gaaggtgaag ggcctgcact ttctgcagga ggacgcccct gatgagatgg gcaagtacat
     4801 caagagcttc gtggagcggg tgctgaagaa cgagcagagc ggaggaggag gctccggcat
     4861 cgagtatggc gataaggagc ggaaggccga cgccaagatg gtgtgcgatg tggtgtccag
     4921 aatggaggac acagagcctt tttctgccga gctgctgagc gccatgatgc ggctgtgggg
     4981 cgactctggc atccaggagt gtttcaaccg gagcagagag taccagctga atgatagcgc
     5041 caagtactat ctggactccc tggatagaat cggcgccgcc gactatcagc caaccgagca
     5101 ggacatcctg cggacacgcg tgaagaccac aggcatcgtg gagacccact tcaccttcaa
     5161 gaacctgcac ttcaggctgt ttgacgtggg aggacagagg tccgagagaa agaagtggat
     5221 tcactgcttc gaggatgtga ccgccatcat cttttgtgtg gccctgtctg gctacgacca
     5281 ggtgctgcac gaggatgaga ccacaaaccg catgcacgag agcctgaagc tgttcgactc
     5341 catctgcaac aataagtggt ttaccgatac aagcatcatc ctgttcctga ataagaagga
     5401 catctttgag gagaagatca agaagagccc cctgaccatc tgtttccctg agtacaccgg
     5461 cccatccgcc tttacagagg ccgtggccta catccaggcc cagtatgagt ctaagaacaa
     5521 gagcgcccac aaggagatct atacccacgt gacatgcgcc accgatacaa acaatatcca
     5581 gttcgtgttt gatgccgtga cagacgtgat catcgctaag aatctgaggg gctgtggcct
     5641 gtattgaggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
     5701 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     5761 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5821 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5881 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5941 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6001 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6421 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6481 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6541 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6601 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6661 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6721 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6781 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6841 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6901 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6961 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7021 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     7081 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7141 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7201 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7261 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7321 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7381 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7441 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7501 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7561 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7621 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7681 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7741 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7801 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7921 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7981 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10141 ccgaaaagtg ccacctgacg tc
//
