LOCUS       sequence-387471-       11101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the
            SunTag-Renilla coding sequence in the Delta-IRE-SunTag reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: IRE-SunTag
FEATURES             Location/Qualifiers
     source          1..11101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3710..3757)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3804..3822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3840..3858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3876..3894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3912..3930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3948..3966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3984..4002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4020..4038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4084..4108
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4367..4423
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4439..4495
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4511..4567
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4583..4639
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4655..4711
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4727..4783
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4799..4855
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4871..4927
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4943..4999
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5015..5071
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5087..5143
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5159..5215
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5231..5287
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5303..5359
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5375..5431
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5447..5503
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5519..5575
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5591..5647
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5663..5719
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5735..5791
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5807..5863
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5879..5935
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6023..6079
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6113..7042
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7043..7045,7046..7366)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7046..7369
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    8899..9033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8977..8996
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9141..9174)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9227..9243)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9259..9281)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9692..10049
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10114..10583
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
     2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
     2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
     2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
     3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
     3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
     3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
     3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
     3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
     3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
     3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
     3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
     3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
     3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
     3781 tttgggccgg ccgaattctt tactccctat cagtgataga gaacgtatga agagtttact
     3841 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     3901 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     3961 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     4021 ccctatcagt gatagagaac gtataaagct ttaggcgtgt acggtgggcg cctataaaag
     4081 cagagctcgt ttagtgaacc gtcagatcgc ctgcttccag acgttcttcg ccgagagtcg
     4141 tcggggtttc ctgcttcaac agtgcttgga cggaacccgg cgctcgttcc ccaccccggc
     4201 cggccgccca tagccagccc tccgtcacct cttcaccgca ccctcggact gccccaaggc
     4261 ccccgccgcc gctccagcgc cgcgcagcca ccgccgccgc cgccgcctct ccttagtcgc
     4321 cgccatgacc ggtccgggtg gatctggagg tggaggttct ggaggagaag aacttttgag
     4381 caagaattat catcttgaga acgaagtggc tcgtcttaag aaaggttctg gcagtggaga
     4441 agaactgctt tcaaagaatt accacctgga aaatgaggta gctagactga aaaaggggag
     4501 cggaagtggg gaggagttgc tgagcaaaaa ttatcatttg gagaacgaag tagcacgact
     4561 aaagaaaggg tccggatcgg gtgaggagtt actctcgaaa aattatcatc tcgaaaacga
     4621 agtggctcgg ctaaaaaagg gcagtggttc tggagaagag ctattatcta aaaactacca
     4681 cctcgaaaat gaggtggcac gcttaaaaaa gggaagtggc agtggtgaag agctactatc
     4741 caagaattat catcttgaga acgaggtagc gcgtttgaag aagggttccg gctcaggaga
     4801 ggaactgctc tcgaagaact atcatcttga aaatgaggtc gctcgattaa aaaagggatc
     4861 gggcagtggt gaggaactac tttcaaagaa ttaccacctc gaaaacgaag tagctcgatt
     4921 aaagaaaggt tcagggtcgg gtgaagaatt actgagtaaa aattatcatc tggaaaatga
     4981 ggtagcgaga ctaaaaaagg ggagtggttc tggcgaagag ttgctatcga aaaattatca
     5041 tcttgagaac gaagttgcta ggctcaaaaa gggctcaggc tcaggcgagg agttgctctc
     5101 gaaaaactac cacttggaaa atgaggtcgc gaggttgaaa aaggggagcg ggtcgggcga
     5161 ggagttattg agcaaaaact atcatttaga gaacgaagtc gcgcgcttaa agaaaggctc
     5221 gggctcgggc gaagaactct tatcgaagaa ctaccacctc gaaaatgagg tcgccaggtt
     5281 gaaaaagggc agtggcagcg gggaggaact cttgagcaag aactaccact tggagaatga
     5341 ggtcgcgaga ttgaagaaag ggtcggggag cggcgaggaa ttgctcagca agaattatca
     5401 tttggagaac gaagtcgcca ggctcaagaa aggctcgggg tcgggggagg aattgttgag
     5461 taaaaactac cacttggaaa atgaagtcgc caggctcaaa aaagggagtg ggagcggcga
     5521 agagttattg agcaaaaatt accacttgga gaacgaagtg gcaaggctca agaaagggag
     5581 cggcagcggg gaggagctct tatcgaagaa ctaccactta gagaatgaag tcgcccgctt
     5641 gaagaaaggc tcggggagcg gggaagagct cttgagcaag aactaccact tggaaaatga
     5701 ggtggcgcgc ttgaagaaag ggagcgggag cggggaagag ttactatcta agaattatca
     5761 tctcgagaac gaggtggctc gactaaagaa gggctccggc agtggggagg aactcctgtc
     5821 gaagaactat catcttgaaa atgaggttgc aagacttaaa aaggggtccg gatcaggtga
     5881 ggaactactc agtaagaatt accacctgga aaacgaagta gctcgattaa agaaaggttc
     5941 agggtcgggt gaagaactgc tctcaaaaga ttatcatttg gaaaatgagg ttgcacgttt
     6001 aaaaaaggga agtggcagtg gtgaggaact tctgtcgaaa aattatcatc tcgagaatga
     6061 agtagcccga cttaaaaagg gttctggctc gggtcagcgg ccgcaccggt cagcttccaa
     6121 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg
     6181 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga
     6241 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt
     6301 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc
     6361 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg
     6421 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg
     6481 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga
     6541 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc
     6601 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac
     6661 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga
     6721 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc
     6781 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct
     6841 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa
     6901 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct
     6961 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga
     7021 gcgcgtgctg aagaacgagc agatgggagt gcaggtggaa accatctccc caggagacgg
     7081 gcgcaccttc cccaagcgcg gccagacctg tgtggtgcac tacaccggga tgcttgaaga
     7141 tggaaagaaa gtcgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
     7201 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
     7261 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
     7321 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ccggaataag ggccagggaa
     7381 gaactggctc ggaaagagga ccccacatgt tgtagcttca agtttaaacg gatccaacct
     7441 acaaacgggt ggaggatcac cccacccgac acttcacaat caaggggtac aatacacaag
     7501 ggtggaggaa caccccaccc tccagacaca ttacacagaa atccaatcaa acagaagcac
     7561 catcagggct tctgctacca aatttatctc aaaaaactac aacaaggaat caccatcagg
     7621 gattccctgt gcaatatacg tcaaacgagg gccacgacgg gaggacgatc acgcctcccg
     7681 aatatcggca tgtctggctt tcgaattcag tgcgtggagc atcagcccac gcagccaatc
     7741 agagtcgaat acaagtcgac tttcgcgaag agcatcagcc ttcgcgccat tcttacacaa
     7801 accacactct cccctacagg aacagcatca gcgttcctgc ccagtaccca actcaagaaa
     7861 atttatgtcc ccatgcagca tcagcgcatg ggccccaaga atacatcccc aacaaaatca
     7921 catccgagca ccaacagggc tcggagtgtt gtttcttgtc caactggaca aaccctccat
     7981 ggaccatcag gccatggact ctcaccaaca agacaaaaac tactcttctc gaagcagcat
     8041 cagcgcttcg aaacactcga gcatacattg tgcctatttc ttgggtggac gatcacgcca
     8101 cccatgctct cacgaatttc aaaacacgga caaggacgag caccaccagg gctcgtcgtt
     8161 ccacgtccaa tacgattact tacctttcgg gatcacgatc acggatcccg cagctacatc
     8221 acttccactc aggacattca agcatgcacg atcacggcat gctccacaag tctcaaccac
     8281 agaaactacc aaatgggttc agcaccagcg aacccactcc tacctcaaac ctcttcccac
     8341 aaaactggca agcaggatca ccgcttgccc attccaacat accaaatcaa aaacaattac
     8401 tggtacagca tcagcgtacc agcccacatc tctcactact atcaaaaacc aaaccgttca
     8461 gcaacagcga acggtacaca cggaaaaatc aactggttta caaatacgaa agacgatcac
     8521 gctttcgtcc agcgcaaact attacgaaaa acatccgacg ggaagagcaa cagccttccc
     8581 gcggcggaaa acctcacaaa aacacgacaa acggatgcac gaacacggca tccgccgaca
     8641 acccacaaac ttacaaccag gcaaacggtg caggatcacc gcaccgtaca tcaaacacct
     8701 cagatctcat ttcgcgcggg atccagacca cctcccctgc gagctaagct ggacagccaa
     8761 tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact
     8821 gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc
     8881 agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     8941 aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta
     9001 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     9061 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     9121 ggtatggctg attatgatct gaagttccta tactatttga agaataggaa cttcggaata
     9181 ggaacttcgg atctcatatg gccatatggg ggcgcctaga gaaggagtga gggctggata
     9241 aagggaggat cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg
     9301 aggaagagga ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa
     9361 agccaaggct tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg
     9421 tgaaaagacc ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg
     9481 ccatccgacg ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt
     9541 gacatctacc gactggttgt gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa
     9601 acttaaggga gtggtaacag gctagcccgg gcccatattt tcagacaaat acagaaacac
     9661 agtcagacag agacaacaca gaacgatgct gcagcagaca agacgcgcgg cgcggcttcg
     9721 gtcccaaacc gaaagcaaaa attcagacgg aggcgggaac tgttttaggt tctcgtctcc
     9781 taccagaacc acatatccct cctctaaggg gggtgcacca aagagtccta aacgatcggg
     9841 attttaggac tcaggtcggg ccacaaaaac ggcccccgaa gtccctggga cgtctcccag
     9901 ggttgcggcc gggtgttccg aactcgtcag ttccaccacg ggtccgccag atacagagct
     9961 agttagctaa ctagtaccga cgcaggcgca taaaatcagt catagacact agacaatcgg
    10021 acagacacag ataagttgct ggccagctta cctcccggtg gtgggtcggt ggtccctggg
    10081 caggggtctc ccgatcccgg acgagccccc aaatgaaaga cccccgctga cgggtagtca
    10141 atcactcaga ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag
    10201 agggtttatt ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgcgccg
    10261 agtgaggggt tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg
    10321 agaagcgaac tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca
    10381 ccctggaaac atctgatggt tctctagaaa ctgctgaggg ctggaccgca tctggggacc
    10441 atctgttctt ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc
    10501 atattcagct gttccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga
    10561 tatcctgttt ggcccatatt cagctgttcc atctgttcct gaccttgatc tgaacttttc
    10621 tattctcagt tatgtatttt tccatgcctt gcaaaatggc gttacttaag ctagcttgcc
    10681 aaacctacag gtggggtctt tcacatgtat atgtcaaaaa taaaaatcaa ctaattgact
    10741 agtaattaat atgactggca taatgggaaa ttgatcctga cagatgcaaa ctggcttctc
    10801 agcagcgcat ttatgttgtc aactgaggaa ggaaacgtta atgacagaaa ctctaagtaa
    10861 tttccacgtt tatctatttt tatttatact agctttggta acaggaatat tgcagcattc
    10921 atgcacattg aaacccttat gaaataaaaa catctgtgca tttaaaatgg aattaacatt
    10981 ttaaatgtta aaaaaagctg gcttagcttc cccccgcccc ctagggcata gaacaagtca
    11041 aatgctttat atatttgagt ttgggatgta ttaggaaact cctaagagca aagctagctt
    11101 g
//
