LOCUS       sequence-387473-       10872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The codon optimized Renilla coding sequence in the IRE-TREAT 
            reporter was replaced by non-optimal Renilla containing 93 
            synonymous codon substitutions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: IRE-nonOpt-TREAT
FEATURES             Location/Qualifiers
     source          1..10872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4086..4110
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8670..8804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8748..8767
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8912..8945)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8998..9014)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9030..9052)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9463..9820
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9885..10354
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataaag ctttaggcgt gtacggtggg cgcctataaa
     4081 agcagagctc gtttagtgaa ccgtcagatc gcctgcttcc agacgttctt cgccgagagt
     4141 cgtcggggtt tcctgcttca acagtgcttg gacggaaccc ggcgctcgtt ccccaccccg
     4201 gccggccgcc catagccagc cctccgtcac ctcttcaccg caccctcgga ctgccccaag
     4261 gcccccgccg ccgctccagc gccgcgcagc caccgccgcc gccgccgcct ctccttagtc
     4321 gccgccatga tggctagtaa ggtgtacgac cccgagcaaa ggaaacgcat gatcactggg
     4381 ccacaatggt gggctaggtg caaacaaatg aacgtgctgg actccttcat aaactactat
     4441 gacagtgaga agcacgccga gaacgccgtg atattcttac atgggaacgc tgcctccagc
     4501 tatctgtgga ggcacgtagt gccgcacatt gaacccgtgg cgagatgcat catacctgat
     4561 ttgataggaa tgggtaagtc cggcaaaagc gggaatggca gctatcgcct cctggatcac
     4621 tacaaatact taactgcgtg gttcgaactg ttaaaccttc cgaaaaaaat catttttgtg
     4681 gggcacgact ggggggcgtg tttagccttt cactacagtt acgagcacca agacaagata
     4741 aaggcgatcg tccatgcgga gagtgtggtg gacgtgatag agagctggga cgagtggccc
     4801 gacattgagg aggacatagc gttaatcaag tcggaagagg gcgagaaaat ggtgcttgag
     4861 aataatttct ttgtagagac catgctcccc agcaagataa tgaggaaatt agagcctgag
     4921 gagttcgcgg cctatctaga gccattcaag gaaaagggcg aggtaagaag gccgacgttg
     4981 agctggcctc gcgaaattcc tctcgttaaa ggaggcaagc ccgacgtcgt ccagattgtt
     5041 aggaactaca acgcgtatct tcgggcgagc gacgatctgc ctaaaatgtt catagagagt
     5101 gaccctgggt tttttagcaa cgctattgta gagggggcta aaaagttccc gaacactgag
     5161 tttgtgaagg tgaaaggctt acatttcagc caggaggacg ctccggatga aatgggtaaa
     5221 tacatcaaga gcttcgtgga aagagtgctg aagaacgagc agtaagtcga cagatcctac
     5281 ctgcaaagca ccagaggata tggcctcggt gcctcagcat cttcagatgt gctgtcactg
     5341 tccaatcttc aagccagcag agcatatggg ctcgctggct ccactgtcat tacagtggac
     5401 ctgatcttct ccaatcaaag gagcagagga tatggcctcg ctccctccga ctacatcagg
     5461 ctggaagtga catccatcat tcatcccagc agagcatatg ggctcgctgg gaccacagca
     5521 tgcctcgaag tgctgagaga ctatcatcaa accgacagag gatatggcct cgtcggctcc
     5581 tactccctcc tggtggaata cttctccact cttcaaccca gcagagcata tgggctcgct
     5641 gggtccatca gagatacaag atctctcgac agatcctacc tgcaaagcac cagaggatat
     5701 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     5761 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     5821 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     5881 catcccagca gagcatatgg gctcgctggg accacagcat gcctcgaagt gctgagagac
     5941 tatcatcaaa ccgacagagg atatggcctc gtcggctcct actccctcct ggtggaatac
     6001 ttctccactc ttcaacccag cagagcatat gggctcgctg ggtccatcag agatacaaga
     6061 tctctcgaca gatcctacct gcaaagcacc agaggatatg gcctcggtgc ctcagcatct
     6121 tcagatgtgc tgtcactgtc caatcttcaa gccagcagag catatgggct cgctggctcc
     6181 actgtcatta cagtggacct gatcttctcc aatcaaagga gcagaggata tggcctcgct
     6241 ccctccgact acatcaggct ggaagtgaca tccatcattc atcccagcag agcatatggg
     6301 ctcgctggga ccacagcatg cctcgaagtg ctgagagact atcatcaaac cgacagagga
     6361 tatggcctcg tcggctccta ctccctcctg gtggaatact tctccactct tcaacccagc
     6421 agagcatatg ggctcgctgg gtccatcaga gatacaagat ctctcgacag atcctacctg
     6481 caaagcacca gaggatatgg cctcggtgcc tcagcatctt cagatgtgct gtcactgtcc
     6541 aatcttcaag ccagcagagc atatgggctc gctggctcca ctgtcattac agtggacctg
     6601 atcttctcca atcaaaggag cagaggatat ggcctcgctc cctccgacta catcaggctg
     6661 gaagtgacat ccatcattca tcccagcaga gcatatgggc tcgctgggac cacagcatgc
     6721 ctcgaagtgc tgagagacta tcatcaaacc gacagaggat atggcctcgt cggctcctac
     6781 tccctcctgg tggaatactt ctccactctt caacccagca gagcatatgg gctcgctggg
     6841 tccatcagag atacaagatc tctcgagccg cggtgcgtaa gtctgttaac agaatgctgc
     6901 atacgctgag tacttcgaaa tgtcatcctc tgtctgacac tgaacgtaat ccagacgcgt
     6961 aagtcaggcc ggaaaattcc cgccaccgga agttgagtag acggtgctgc ctgcgactca
     7021 accccaggag gactgggtga acaaagctgc gaagtgatcc atgtaagccc tcagaaccgt
     7081 ctcggaaaga ggaccccaca tgttgtagct tcaaggccca atgtcagacc acgccatggc
     7141 gtgccactct gcggagagtg cagtctgcga cagtgcccca ggaggactgg gtgaggatcc
     7201 tacctacaaa cggcacgagc atcagccgtg ccgcccagca tcttcatcac tgtccaatct
     7261 tcaagcgtgc acgatcacgg cacgctccac tgtcattaca gtcttatcca atcaaactcc
     7321 agcagcatca gcgctggagc tccgactaca tcaggacatc catcattcaa cgctcgacga
     7381 tcacgcgagc gtccacagca tgcctcagac actatcatca aacggctgca cgatcacggc
     7441 agccgctcct actgcctcta cgttatcgac tcttctgcga cgagcatcag ccgtcgcaac
     7501 atcggacata ccagatccta cctacaaacg gcacgagcat cagccgtgcc gcccagcatc
     7561 ttcatcactg tccaatcttc aagcgtgcac gatcacggca cgctccactg tcattacagt
     7621 cttatccaat caaactccag cagcatcagc gctggagctc cgactacatc aggacatcca
     7681 tcattcaacg ctcgacgatc acgcgagcgt ccacagcatg cctcagacac tatcatcaaa
     7741 cggctgcacg atcacggcag ccgctcctac tgcctctacg ttatcgactc ttctgcgacg
     7801 agcatcagcc gtcgcaacat cggacatacc agatcctacc tacaaacggc acgagcatca
     7861 gccgtgccgc ccagcatctt catcactgtc caatcttcaa gcgtgcacga tcacggcacg
     7921 ctccactgtc attacagtct tatccaatca aactccagca gcatcagcgc tggagctccg
     7981 actacatcag gacatccatc attcaacgct cgacgatcac gcgagcgtcc acagcatgcc
     8041 tcagacacta tcatcaaacg gctgcacgat cacggcagcc gctcctactg cctctacgtt
     8101 atcgactctt ctgcgacgag catcagccgt cgcaacatcg gacataccag atcctaccta
     8161 caaacggcac gagcatcagc cgtgccgccc agcatcttca tcactgtcca atcttcaagc
     8221 gtgcacgatc acggcacgct ccactgtcat tacagtctta tccaatcaaa ctccagcagc
     8281 atcagcgctg gagctccgac tacatcagga catccatcat tcaacgctcg acgatcacgc
     8341 gagcgtccac agcatgcctc agacactatc atcaaacggc tgcacgatca cggcagccgc
     8401 tcctactgcc tctacgttat cgactcttct gcgacgagca tcagccgtcg caacatcgga
     8461 cataccagat cttcaatcga tttcgcgcgg gatccagacc acctcccctg cgagctaagc
     8521 tggacagcca atgacgggta agagagtgac atttttcact aacctaagac aggagggccg
     8581 tcagagctac tgcctaatcc aaagacgggt aaaagtgata aaaatgtatc actccaacct
     8641 aagacaggcg cagcttccga gggatttgag atccagacat gataagatac attgatgagt
     8701 ttggacaaac caaaactaga atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg
     8761 ctattgcctt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     8821 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     8881 tctacaaatg tggtatggct gattatgatc tgaagttcct atactatttg aagaatagga
     8941 acttcggaat aggaacttcg gatctcatat ggccatatgg gggcgcctag agaaggagtg
     9001 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
     9061 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
     9121 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     9181 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
     9241 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
     9301 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
     9361 cctaaggtca aacttaaggg agtggtaaca ggctagcccg ggcccatatt ttcagacaaa
     9421 tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg
     9481 gcgcggcttc ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg
     9541 ttctcgtctc ctaccagaac cacatatccc tcctctaagg ggggtgcacc aaagagtcct
     9601 aaacgatcgg gattttagga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg
     9661 acgtctccca gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca
     9721 gatacagagc tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac
     9781 tagacaatcg gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg
     9841 tggtccctgg gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg
     9901 acgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat
     9961 gcaactgcaa gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga
    10021 ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc
    10081 gaacagaagc gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg
    10141 tccttggggc accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc
    10201 atctggggac catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc
    10261 ctgtttggcc catattcagc tgttccatct gttcttggcc ctgagccggg gcaggaactg
    10321 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttcc tgaccttgat
    10381 ctgaactttt ctattctcag ttatgtattt ttccatgcct tgcaaaatgg cgttacttaa
    10441 gctagcttgc caaacctaca ggtggggtct ttcacatgta tatgtcaaaa ataaaaatca
    10501 actaattgac tagtaattaa tatgactggc ataatgggaa attgatcctg acagatgcaa
    10561 actggcttct cagcagcgca tttatgttgt caactgagga aggaaacgtt aatgacagaa
    10621 actctaagta atttccacgt ttatctattt ttatttatac tagctttggt aacaggaata
    10681 ttgcagcatt catgcacatt gaaaccctta tgaaataaaa acatctgtgc atttaaaatg
    10741 gaattaacat tttaaatgtt aaaaaaagct ggcttagctt ccccccgccc cctagggcat
    10801 agaacaagtc aaatgcttta tatatttgag tttgggatgt attaggaaac tcctaagagc
    10861 aaagctagct tg
//
