LOCUS       sequence-387475-        6907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cre recombinase under the control of the tryptophan 
            hydroxylase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, 
            Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, Banala S, 
            Freeman M, Xu CS, Kirchhausen T, Hess HF, Lavis L, Li Y, 
            Chaumont-Dubel S, Clapham DE
  TITLE     A serotonergic axon-cilium synapse drives nuclear signaling to alter
            chromatin accessibility.
  JOURNAL   Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 
            10.1016/j.cell.2022.07.026.
  PUBMED    36055200
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-09-1- 1";
            volume: "185"; issue: "18"; pages: "3390-3407.e18"
COMMENT     Sequence Label: pAAV-TPH2-Cre
FEATURES             Location/Qualifiers
     source          1..6907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   13..153
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   24..153
                     /label=AAV2 ITR
     protein_bind    160..180
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2222..2246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2247..2256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2259..2279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2280..3308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     protein_bind    complement(3309..3333)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3343..3931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3396..3416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3814..3825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3961..3978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3967..4174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4182..4322
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4182..4311
                     /label=AAV2 ITR
     rep_origin      4397..4852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4484..4503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4694..4715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4869..4888)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4988..5010
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5048..5066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5134..5238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5239..5307,5308..6099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5457..5476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6270..6858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6759..6778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
       61 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      121 agggagtggc caactccatc actaggggtt ccttctagac aactttgtat agaaaagttg
      181 agatctggac tgaccactcc gtgcctttgc ttttcgtgtc ttctgcagag aggtcgatac
      241 cctttgttct acttggttat cgcacaactt gattctctca gccagactgt gatctccgtg
      301 ggtcagtgct gcatcgaaca cttctttgca tgcttgggat tcagtttcag cttactagaa
      361 actcagggct caacaaaatt ttgttgagtt atacatctgt tctgtgtctg gctctgtagt
      421 ctgtcgagtc tgcttttctt tacacccttc tccttctttg gaagagaaca cggattccca
      481 aagtcaaatt gactgaatta agatcctacc ttagacattc ttttgtgcct tagtacacac
      541 acacacatac acacacacac acacacacac acacagatgc acacacatac acacatatgc
      601 acacacacat acacacagat gcacacacac acatacacat acacacagat gcacacacgc
      661 atacacatac acacagatgc acacacacat acacatacac acagatgcac acacgcacac
      721 acatacacat acacacatgc acacgcacac ataaacatac acacagatgc acacacgcac
      781 acacataaac atacacatgc acacacacac acacatgcac acacacatgc acatgcacac
      841 acacacacac acaccagttt tatgataact gactatgttc tgacctataa acgtgcataa
      901 aaatccagaa tatgattact tagcattgtt agctattatt ttttacaatt acttcagtta
      961 atttattaat aatttctctt cctcttagcc tcttagtctt cagaggaaat aggagctaaa
     1021 tgattatttc cagagttgga tcaaataata atcctaaacc ctcaagtctc cagaaacagt
     1081 taaaatccaa ccaatgttaa catatacaga cttgttttga agaatcttca ttttgttttc
     1141 tatacaatcc aattgtacga gtcccagatt ttgtttagtt cttcagttaa acatgtactc
     1201 ttggttaaga attttccatg tttaaaagtt atttgttatt tttacgtaca tgagtacact
     1261 gtagctgtct tcatacacac cagaagaggg catcaaatcc ccattacata tgattgtgag
     1321 ccacctagtg gttgctggga tttgaactca ggacctctgg aagagcagtc agcactctta
     1381 accactaagc tatctctcca gccccaaact ttccattctt taaaggattg ctaagctttc
     1441 ccgtggcttt ctaaagttgg gaaggtacct atagaatttt gtctatgcct gtcagattgc
     1501 tgggtctgat taagttatag atggggagca gcagaattgc atcagaaaag catcaaaggg
     1561 atagtgggcc tatgggcatt tcatttccat atgtttctta cttagtgaag aacattctag
     1621 aaggtgagca tcttggctaa gttcaagcct cgaatttcaa tagcctgagt aaccttctac
     1681 ctggagacac cacaggtttt ggaaacaggc ctcttacgaa agccatcaca catacataca
     1741 cataaaattt gcacatcgta acatgtcatg gtgcagaaaa gccttcctta agctattttc
     1801 ttacttttag aaagcacaaa gcactcaaac tcatagttac tggaagactg agggtaacct
     1861 tccaatagca ggccctagaa ttaaaattag acgactgcta ttttgttgca aggaaaaaaa
     1921 taataataaa ccctgacgca tagctctcca ccatctcttc ccaaagggct actcgaccta
     1981 tgaaacaaac aaatctcatc atgagcacag ataaccccag gcttcagacg tgtaatctga
     2041 ttgtggccat cagcaaccag aaatgagttt tttctaatca gcttcccatc actcctcagt
     2101 cactcatata aaggaacaca gggaggggag gaagcgcact gctcttcagc accagggttc
     2161 tggacagcgc ctcgagcagc cagctgccgc tcaccttcct cctacatctc tgccgcaccg
     2221 gtcaagtttg tacaaaaaag caggctgcca ccatgggccc aaagaagaag agaaaggttt
     2281 cgaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
     2341 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
     2401 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
     2461 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
     2521 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
     2581 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
     2641 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
     2701 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
     2761 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
     2821 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
     2881 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
     2941 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
     3001 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     3061 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     3121 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     3181 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca
     3241 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     3301 gcgattagac ccagctttct tgtacaaagt gggaattccg ataatcaacc tctggattac
     3361 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3721 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3901 agtcggatct ccctttgggc cgcctccccg catcgggaat tcctagagct cgctgatcag
     3961 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4021 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4081 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4141 aggattggga agagaatagc aggcatgctg gggagggccg caggaacccc tagtgatgga
     4201 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4261 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     4321 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4381 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4441 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
     4501 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4561 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4621 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4681 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     4741 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     4801 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4861 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4921 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4981 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5041 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5101 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5221 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5281 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5341 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5401 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5461 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5521 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5581 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5641 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5701 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5761 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5821 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5881 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5941 gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6001 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6061 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6121 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6181 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6901 tggcctt
//
