LOCUS       sequence-387478-       11213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich 
            stem-loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: SL-SunTag
FEATURES             Location/Qualifiers
     source          1..11213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3710..3757)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3804..3822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3840..3858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3876..3894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3912..3930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3948..3966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3984..4002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4020..4038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          4205..4337
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4320..4339
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4382..4400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4404..4413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4479..4535
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4551..4607
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4623..4679
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4695..4751
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4767..4823
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4839..4895
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4911..4967
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4983..5039
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5055..5111
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5127..5183
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5199..5255
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5271..5327
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5343..5399
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5415..5471
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5487..5543
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5559..5615
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5631..5687
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5703..5759
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5775..5831
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5847..5903
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5919..5975
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5991..6047
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6135..6191
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6225..7154
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7155..7157,7158..7478)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7158..7481
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    9011..9145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9089..9108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9253..9286)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9339..9355)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9371..9393)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9804..10161
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10226..10695
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
     2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
     2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
     2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
     3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
     3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
     3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
     3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
     3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
     3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
     3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
     3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
     3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
     3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
     3781 tttgggccgg ccgaattctt tactccctat cagtgataga gaacgtatga agagtttact
     3841 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     3901 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     3961 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     4021 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
     4081 agagctcgtt tagtgaaccg tcagatgcgc cggtgcagcc ggcgcagcgc ctggagcaat
     4141 tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaaaccgg
     4201 tcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
     4261 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     4321 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
     4381 ttaatacgac tcactatagg ctagccacca tgggcgccat catacacgcg gccgcaatca
     4441 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     4501 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     4561 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     4621 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     4681 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     4741 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     4801 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     4861 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     4921 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     4981 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     5041 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     5101 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     5161 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     5221 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     5281 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     5341 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     5401 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     5461 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     5521 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     5581 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     5641 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     5701 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     5761 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     5821 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     5881 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     5941 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     6001 tcagtaagaa ttaccacctg gaaaacgaag tagctcgatt aaagaaaggt tcagggtcgg
     6061 gtgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     6121 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     6181 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcttcc aaggtgtacg
     6241 accccgagca acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa
     6301 tgaacgtgct ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg
     6361 tgatttttct gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca
     6421 tcgagcccgt ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga
     6481 gcgggaatgg ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc
     6541 tgctgaacct tccaaagaaa atcatctttg tgggccacga ctggggggct tgtctggcct
     6601 ttcactactc ctacgagcac caagacaaga tcaaggccat cgtccatgct gagagtgtcg
     6661 tggacgtgat cgagtcctgg gacgagtggc ctgacatcga ggaggatatc gccctgatca
     6721 agagcgaaga gggcgagaaa atggtgcttg agaataactt cttcgtcgag accatgctcc
     6781 caagcaagat catgcggaaa ctggagcctg aggagttcgc tgcctacctg gagccattca
     6841 aggagaaggg cgaggttaga cggcctaccc tctcctggcc tcgcgagatc cctctcgtta
     6901 agggaggcaa gcccgacgtc gtccagattg tccgcaacta caacgcctac cttcgggcca
     6961 gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg gttcttttcc aacgctattg
     7021 tcgagggagc taagaagttc cctaacaccg agttcgtgaa ggtgaagggc ctccacttca
     7081 gccaggagga cgctccagat gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc
     7141 tgaagaacga gcagatggga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct
     7201 tccccaagcg cggccagacc tgtgtggtgc actacaccgg gatgcttgaa gatggaaaga
     7261 aagtcgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg
     7321 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga
     7381 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg
     7441 ccactctcgt cttcgatgtg gagcttctaa aaccggaata agggccaggg aagaactggc
     7501 tcggaaagag gaccccacat gttgtagctt caagtttaaa cggatccaac ctacaaacgg
     7561 gtggaggatc accccacccg acacttcaca atcaaggggt acaatacaca agggtggagg
     7621 aacaccccac cctccagaca cattacacag aaatccaatc aaacagaagc accatcaggg
     7681 cttctgctac caaatttatc tcaaaaaact acaacaagga atcaccatca gggattccct
     7741 gtgcaatata cgtcaaacga gggccacgac gggaggacga tcacgcctcc cgaatatcgg
     7801 catgtctggc tttcgaattc agtgcgtgga gcatcagccc acgcagccaa tcagagtcga
     7861 atacaagtcg actttcgcga agagcatcag ccttcgcgcc attcttacac aaaccacact
     7921 ctcccctaca ggaacagcat cagcgttcct gcccagtacc caactcaaga aaatttatgt
     7981 ccccatgcag catcagcgca tgggccccaa gaatacatcc ccaacaaaat cacatccgag
     8041 caccaacagg gctcggagtg ttgtttcttg tccaactgga caaaccctcc atggaccatc
     8101 aggccatgga ctctcaccaa caagacaaaa actactcttc tcgaagcagc atcagcgctt
     8161 cgaaacactc gagcatacat tgtgcctatt tcttgggtgg acgatcacgc cacccatgct
     8221 ctcacgaatt tcaaaacacg gacaaggacg agcaccacca gggctcgtcg ttccacgtcc
     8281 aatacgatta cttacctttc gggatcacga tcacggatcc cgcagctaca tcacttccac
     8341 tcaggacatt caagcatgca cgatcacggc atgctccaca agtctcaacc acagaaacta
     8401 ccaaatgggt tcagcaccag cgaacccact cctacctcaa acctcttccc acaaaactgg
     8461 caagcaggat caccgcttgc ccattccaac ataccaaatc aaaaacaatt actggtacag
     8521 catcagcgta ccagcccaca tctctcacta ctatcaaaaa ccaaaccgtt cagcaacagc
     8581 gaacggtaca cacggaaaaa tcaactggtt tacaaatacg aaagacgatc acgctttcgt
     8641 ccagcgcaaa ctattacgaa aaacatccga cgggaagagc aacagccttc ccgcggcgga
     8701 aaacctcaca aaaacacgac aaacggatgc acgaacacgg catccgccga caacccacaa
     8761 acttacaacc aggcaaacgg tgcaggatca ccgcaccgta catcaaacac ctcagatctc
     8821 atttcgcgcg ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
     8881 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
     8941 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
     9001 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     9061 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     9121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     9181 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     9241 tgattatgat ctgaagttcc tatactattt gaagaatagg aacttcggaa taggaacttc
     9301 ggatctcata tggccatatg ggggcgccta gagaaggagt gagggctgga taaagggagg
     9361 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
     9421 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
     9481 cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga
     9541 ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga
     9601 cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta
     9661 ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg
     9721 gagtggtaac aggctagccc gggcccatat tttcagacaa atacagaaac acagtcagac
     9781 agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa
     9841 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
     9901 ccacatatcc ctcctctaag gggggtgcac caaagagtcc taaacgatcg ggattttagg
     9961 actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc agggttgcgg
    10021 ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
    10081 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
    10141 agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc
    10201 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
    10261 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
    10321 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg
    10381 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
    10441 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
    10501 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
    10561 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
    10621 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
    10681 ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttt tctattctca
    10741 gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
    10801 aggtggggtc tttcacatgt atatgtcaaa aataaaaatc aactaattga ctagtaatta
    10861 atatgactgg cataatggga aattgatcct gacagatgca aactggcttc tcagcagcgc
    10921 atttatgttg tcaactgagg aaggaaacgt taatgacaga aactctaagt aatttccacg
    10981 tttatctatt tttatttata ctagctttgg taacaggaat attgcagcat tcatgcacat
    11041 tgaaaccctt atgaaataaa aacatctgtg catttaaaat ggaattaaca ttttaaatgt
    11101 taaaaaaagc tggcttagct tccccccgcc ccctagggca tagaacaagt caaatgcttt
    11161 atatatttga gtttgggatg tattaggaaa ctcctaagag caaagctagc ttg
//
