LOCUS       sequence-387481-       11013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mir21 binding sites (3x) were integrated in between the stop codon 
            and TREAT cassette in IRE-TREAT reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: miR21-IRE-TREAT
FEATURES             Location/Qualifiers
     source          1..11013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4086..4110
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4330..5265
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8811..8945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8889..8908
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9053..9086)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9139..9155)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9171..9193)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9604..9961
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10026..10495
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataaag ctttaggcgt gtacggtggg cgcctataaa
     4081 agcagagctc gtttagtgaa ccgtcagatc gcctgcttcc agacgttctt cgccgagagt
     4141 cgtcggggtt tcctgcttca acagtgcttg gacggaaccc ggcgctcgtt ccccaccccg
     4201 gccggccgcc catagccagc cctccgtcac ctcttcaccg caccctcgga ctgccccaag
     4261 gcccccgccg ccgctccagc gccgcgcagc caccgccgcc gccgccgcct ctccttagtc
     4321 gccgccatga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     4381 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     4441 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     4501 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     4561 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     4621 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     4681 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     4741 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     4801 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     4861 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     4921 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     4981 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     5041 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     5101 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     5161 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     5221 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaagggcc agggaagaac
     5281 tgatcgccgt gtaaccgcgg agttgttcag agacataagc tatcggaagt tgttcagaga
     5341 cataagctag gcctagttgt tcagagacat aagctaccgc ggcgcgcgcg ggatccggat
     5401 ccaacctaca aaagatccta cctgcaaagc accagaggat atggcctcgg tgcctcagca
     5461 tcttcagatg tgctgtcact gtccaatctt caagccagca gagcatatgg gctcgctggc
     5521 tccactgtca ttacagtgga cctgatcttc tccaatcaaa ggagcagagg atatggcctc
     5581 gctccctccg actacatcag gctggaagtg acatccatca ttcatcccag cagagcatat
     5641 gggctcgctg ggaccacagc atgcctcgaa gtgctgagag actatcatca aaccgacaga
     5701 ggatatggcc tcgtcggctc ctactccctc ctggtggaat acttctccac tcttcaaccc
     5761 agcagagcat atgggctcgc tgggtccatc agagatacaa gatctctcga cagatcctac
     5821 ctgcaaagca ccagaggata tggcctcggt gcctcagcat cttcagatgt gctgtcactg
     5881 tccaatcttc aagccagcag agcatatggg ctcgctggct ccactgtcat tacagtggac
     5941 ctgatcttct ccaatcaaag gagcagagga tatggcctcg ctccctccga ctacatcagg
     6001 ctggaagtga catccatcat tcatcccagc agagcatatg ggctcgctgg gaccacagca
     6061 tgcctcgaag tgctgagaga ctatcatcaa accgacagag gatatggcct cgtcggctcc
     6121 tactccctcc tggtggaata cttctccact cttcaaccca gcagagcata tgggctcgct
     6181 gggtccatca gagatacaag atctctcgac agatcctacc tgcaaagcac cagaggatat
     6241 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     6301 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     6361 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     6421 catcccagca gagcatatgg gctcgctggg accacagcat gcctcgaagt gctgagagac
     6481 tatcatcaaa ccgacagagg atatggcctc gtcggctcct actccctcct ggtggaatac
     6541 ttctccactc ttcaacccag cagagcatat gggctcgctg ggtccatcag agatacaaga
     6601 tctctcgaca gatcctacct gcaaagcacc agaggatatg gcctcggtgc ctcagcatct
     6661 tcagatgtgc tgtcactgtc caatcttcaa gccagcagag catatgggct cgctggctcc
     6721 actgtcatta cagtggacct gatcttctcc aatcaaagga gcagaggata tggcctcgct
     6781 ccctccgact acatcaggct ggaagtgaca tccatcattc atcccagcag agcatatggg
     6841 ctcgctggga ccacagcatg cctcgaagtg ctgagagact atcatcaaac cgacagagga
     6901 tatggcctcg tcggctccta ctccctcctg gtggaatact tctccactct tcaacccagc
     6961 agagcatatg ggctcgctgg gtccatcaga gatacaagat ctctcgagcc gcggtgcgta
     7021 agtctgttaa cagaatgctg catacgctga gtacttcgaa atgtcatcct ctgtctgaca
     7081 ctgaacgtaa tccagacgcg taagtcaggc cggaaaattc ccgccaccgg aagttgagta
     7141 gacggtgctg cctgcgactc aaccccagga ggactgggtg aacaaagctg cgaagtgatc
     7201 catgtaagcc ctcagaaccg tctcggaaag aggaccccac atgttgtagc ttcaaggccc
     7261 aatgtcagac cacgccatgg cgtgccactc tgcggagagt gcagtctgcg acagtgcccc
     7321 aggaggactg ggtgaggatc ctacctacaa acggcacgag catcagccgt gccgcccagc
     7381 atcttcatca ctgtccaatc ttcaagcgtg cacgatcacg gcacgctcca ctgtcattac
     7441 agtcttatcc aatcaaactc cagcagcatc agcgctggag ctccgactac atcaggacat
     7501 ccatcattca acgctcgacg atcacgcgag cgtccacagc atgcctcaga cactatcatc
     7561 aaacggctgc acgatcacgg cagccgctcc tactgcctct acgttatcga ctcttctgcg
     7621 acgagcatca gccgtcgcaa catcggacat accagatcct acctacaaac ggcacgagca
     7681 tcagccgtgc cgcccagcat cttcatcact gtccaatctt caagcgtgca cgatcacggc
     7741 acgctccact gtcattacag tcttatccaa tcaaactcca gcagcatcag cgctggagct
     7801 ccgactacat caggacatcc atcattcaac gctcgacgat cacgcgagcg tccacagcat
     7861 gcctcagaca ctatcatcaa acggctgcac gatcacggca gccgctccta ctgcctctac
     7921 gttatcgact cttctgcgac gagcatcagc cgtcgcaaca tcggacatac cagatcctac
     7981 ctacaaacgg cacgagcatc agccgtgccg cccagcatct tcatcactgt ccaatcttca
     8041 agcgtgcacg atcacggcac gctccactgt cattacagtc ttatccaatc aaactccagc
     8101 agcatcagcg ctggagctcc gactacatca ggacatccat cattcaacgc tcgacgatca
     8161 cgcgagcgtc cacagcatgc ctcagacact atcatcaaac ggctgcacga tcacggcagc
     8221 cgctcctact gcctctacgt tatcgactct tctgcgacga gcatcagccg tcgcaacatc
     8281 ggacatacca gatcctacct acaaacggca cgagcatcag ccgtgccgcc cagcatcttc
     8341 atcactgtcc aatcttcaag cgtgcacgat cacggcacgc tccactgtca ttacagtctt
     8401 atccaatcaa actccagcag catcagcgct ggagctccga ctacatcagg acatccatca
     8461 ttcaacgctc gacgatcacg cgagcgtcca cagcatgcct cagacactat catcaaacgg
     8521 ctgcacgatc acggcagccg ctcctactgc ctctacgtta tcgactcttc tgcgacgagc
     8581 atcagccgtc gcaacatcgg acataccaga tcttcaatcg atttcgcgcg ggatccagac
     8641 cacctcccct gcgagctaag ctggacagcc aatgacgggt aagagagtga catttttcac
     8701 taacctaaga caggagggcc gtcagagcta ctgcctaatc caaagacggg taaaagtgat
     8761 aaaaatgtat cactccaacc taagacaggc gcagcttccg agggatttga gatccagaca
     8821 tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc
     8881 ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac
     8941 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     9001 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctgaagttcc
     9061 tatactattt gaagaatagg aacttcggaa taggaacttc ggatctcata tggccatatg
     9121 ggggcgccta gagaaggagt gagggctgga taaagggagg atcgaggcgg ggtcgaacga
     9181 ggaggttcaa gggggagaga cggggcggat ggaggaagag gaggcggagg cttagggtgt
     9241 acaaagggct tgacccaggg aggggggtca aaagccaagg cttcccaggt cacgatgtag
     9301 gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg
     9361 atgaggtctc ggttaaaggt gccgtctcgc ggccatccga cgttaaaggt tggccattct
     9421 gcagagcaga aggtaaccca acgtctcttc ttgacatcta ccgactggtt gtgagcgatc
     9481 cgctcgacat ctttccagtg acctaaggtc aaacttaagg gagtggtaac aggctagccc
     9541 gggcccatat tttcagacaa atacagaaac acagtcagac agagacaaca cagaacgatg
     9601 ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa ccgaaagcaa aaattcagac
     9661 ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc ctcctctaag
     9721 gggggtgcac caaagagtcc taaacgatcg ggattttagg actcaggtcg ggccacaaaa
     9781 acggcccccg aagtccctgg gacgtctccc agggttgcgg ccgggtgttc cgaactcgtc
     9841 agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc gacgcaggcg
     9901 cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg ctggccagct
     9961 tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc ggacgagccc
    10021 ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa
    10081 cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg
    10141 gcgactcagt caatcggagg actggcgcgc cgagtgaggg gttgtgggct cttttattga
    10201 gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat
    10261 aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga
    10321 aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag
    10381 gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc
    10441 cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt
    10501 ccatctgttc ctgaccttga tctgaacttt tctattctca gttatgtatt tttccatgcc
    10561 ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc tttcacatgt
    10621 atatgtcaaa aataaaaatc aactaattga ctagtaatta atatgactgg cataatggga
    10681 aattgatcct gacagatgca aactggcttc tcagcagcgc atttatgttg tcaactgagg
    10741 aaggaaacgt taatgacaga aactctaagt aatttccacg tttatctatt tttatttata
    10801 ctagctttgg taacaggaat attgcagcat tcatgcacat tgaaaccctt atgaaataaa
    10861 aacatctgtg catttaaaat ggaattaaca ttttaaatgt taaaaaaagc tggcttagct
    10921 tccccccgcc ccctagggca tagaacaagt caaatgcttt atatatttga gtttgggatg
    10981 tattaggaaa ctcctaagag caaagctagc ttg
//
