LOCUS       sequence-387483-        5685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the protein of emiRFP670 and HO1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pBAD-HisD-emiRFP670-HO1
FEATURES             Location/Qualifiers
     source          1..5685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             328..345
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2086..2103)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2256..2342
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2434..2461
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2480..2571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2572..2640,2641..3432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2790..2809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3603..4191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4092..4111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4345..4362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4377..4517
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4443..4462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4603..4625
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4781..5659)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggcc
      361 gagggcagcg tggccagaca gcccgacctg ctgacctgcg aacatgaaga gatccacctc
      421 gccggctcga tccagccgca tggcgcgctt ctggtcgtca gcgaacatga tcatcgcgtc
      481 atccaggcca gcgccaacgc cgcggaattt ctgaatctcg gaagcgtact cggcgttccg
      541 ctcgccgaga tcgacggcga tctgttgatc aagatcctgc cgcatctcga tcccaccgcc
      601 gaaggcatgc cggtcgcggt gcgctgccgg atcggcaatc cctctacgga gtactgcggt
      661 ctgatgcatc ggcctccgga aggcgggctg atcatcgaac tcgaacgtgc cggcccgtcg
      721 atcgatctgt caggcacgct ggcgccggcg ctggagcgga tccgcacggc gggttcactg
      781 cgcgcgctgt gcgatgacac cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg
      841 atggtgtatc gtttcgatga gcaaggccac ggcctggtat tctccgagtg ccatgtgcct
      901 gggctcgaat cctatttcgg caaccgctat ccgtcgtcga ctgtcccgca gatggcgcgg
      961 cagctgtacg tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg
     1021 ctggagccgc ggctgtcgcc gctgaccggg cgcgatctcg acatgtcggg ctgcttcctg
     1081 cgctcgatgt cgccgtgcca tctgcagttc ctgaaggaca tgggcgtgcg cgccaccctg
     1141 gcggtgtcgc tggtggtcgg cggcaagctg tggggcctgg ttgtctgtca ccattatctg
     1201 ccgcgcttca tccgtttcga gctgcgggcg atctgcaaac ggctcgccga aaggatcgcg
     1261 acgcggatca ccgcgcttga gagctaatag ctgcagaaga aggagatata catacccatg
     1321 agtgtcaact tagcttccca gttgcgggaa gggacgaaaa aatcccactc catggcggag
     1381 aacgtcggct ttgtcaaatg cttcctcaag ggcgttgtcg agaaaaattc ctaccgtaag
     1441 ctggttggca atctctactt tgtctacagt gccatggaag aggaaatggc aaaatttaag
     1501 gaccatccca tcctcagcca catttacttc cccgaactca accgcaaaca aagcctagag
     1561 caagacctgc aattctatta cggctccaac tggcggcaag aagtgaaaat ttctgccgct
     1621 ggccaagcct atgtggaccg agtccggcaa gtggccgcta cggcccctga attgttggtg
     1681 gcccattcct acacccgtta cctgggggat ctttccggcg gtcaaattct caagaaaatt
     1741 gcccaaaatg ccatgaatct ccacgatggt ggcacagctt tctatgaatt tgccgacatt
     1801 gatgacgaaa aggcttttaa aaatacctac cgtcaagcta tgaatgatct gcccattgac
     1861 caagccaccg ccgaacggat tgtggatgaa gccaatgacg cctttgccat gaacatgaaa
     1921 atgttcaacg aacttgaagg caacctgatc aaggcgatcg gcattatggt gttcaacagc
     1981 ctcacccgtc gccgcagtca aggcagcacc gaagttggcc tcgccacctc cgaaggctaa
     2041 gaattcgaag cttggctgtt ttggtggatg agagaagatt ttcagcctga tacagattaa
     2101 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     2161 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     2221 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     2281 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     2341 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     2401 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     2461 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3721 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4261 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4381 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4441 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4501 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4561 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4621 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     4681 cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat
     4741 gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta
     4801 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
     4861 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
     4921 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
     4981 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
     5041 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
     5101 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
     5161 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
     5221 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
     5281 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
     5341 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
     5401 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
     5461 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
     5521 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
     5581 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
     5641 cattttgcgc ttcagccata cttttcatac tcccgccatt cagag
//
