LOCUS       sequence-387495-        8414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express hM4D/2A/GFP in 
            the presence of Cre and the leakage expression is significantly 
            reduced without Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Matsushita N, Kato S, Nishizawa K, Sugawara M, Takeuchi K, Miyasaka 
            Y, Mashimo T, Kobayashi K
  TITLE     Highly selective transgene expression through the flip-excision 
            switch system by using a unilateral spacer sequence.
  JOURNAL   Cell Rep Methods. 2023 Jan 18;3(2):100393. doi: 
            10.1016/j.crmeth.2022.100393. eCollection 2023 Feb 27.
  PUBMED    36936079
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-01-18"; volume: "3(2):100393. doi"; pages: " 
            10.1016/j.crmeth.2022.100393. eCollection 2023 Feb 27"
COMMENT     Sequence Label: pAAV-EF1-alpha-Flex/3'USS-hM4D/2A/GFP(ATG mut)
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   64..193
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        211..1389
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          442..1380
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1337..1357
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1395..1428)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1479..1512)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(1531..2244,2245..2247,2248..2250))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1534..2244,2245..2247,2248..2250))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1576..1597)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1925..1944
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2184..2205
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2251..2304)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      3747..3756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3791..3824
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3850..4554
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="VGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTMK
                     IKVVEGGPLPFAFDILATSF*YGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGGV
                     LTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQM
                     ALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAVA
                     RYCDLPSKLGHS"
     protein_bind    4575..4608
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    4624..5212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4677..4697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5095..5106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5224..5700
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5423..5442)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5740..5880
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5740..5869
                     /label=AAV2 ITR
     rep_origin      5955..6410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6042..6061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6252..6273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6427..6446)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6546..6568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6606..6624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6692..6796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6797..6865,6866..7657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7015..7034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7828..8414,1..2)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8317..8336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc
      121 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      181 actaggggtt cctgcggccg cacgccgtga ggctccggtg cccgtcagtg ggcagagcgc
      241 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      301 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      361 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      421 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      481 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      541 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      601 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      661 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      721 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      781 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      841 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      901 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
      961 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     1021 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     1081 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1141 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1201 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1261 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1321 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1381 gtgtcgtgaa attcataact tcgtatagca tacattatac gaagttatgc agaatggtag
     1441 ctggattgta gctgctatta gcaatatgaa acctcttaat aacttcgtat aggatacttt
     1501 atacgaagtt atggatcctc tagagtcgac ttacttgtac agctcgtcca tgccgagagt
     1561 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     1621 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
     1681 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     1741 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     1801 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     1861 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     1921 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
     1981 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
     2041 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
     2101 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
     2161 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
     2221 ggtgaacagc tcctcgccct tgctcaccat tgggccagga ttctcctcga cgtcaccgca
     2281 tgttagcaga cttcctctgc cctctccact gcccctggca gtgccgatgt tccgatactg
     2341 gcacagcagc aggtgccgga aggtcttttt aaaggtggcg ttgcacagag catagcaggc
     2401 agggttgatg gtgctgttga cgtagcagag ccagtagcca atggaccaca ccgtgtcagg
     2461 gatgcagctc tggcagaagg tgttcaccag gaccatgacg ttgtagggcg tccaggtgag
     2521 gatgaaggcc agcagaatgg caaagatcgt tcgtgtcact ttgcgctccc gggccgccat
     2581 ctgccgcttc ttgcgcacct ggttgcgagc gatgctggcg aacttgcggg ccacgttggc
     2641 cgcagggcgc atgccagccg gcgtggcagg cacaatctca atggctgtca cacactcatt
     2701 gcctgtctgc ttcgtcacaa tctggatctt ggaccatctg gaggctgggt tgagggcccg
     2761 cggctgcagg ggaggggcgg gcatggcggg cgtggtggcc tctgtggtgg acagctctgt
     2821 ggctgggcgt tccttggtgt tctgggtggc actgcctgag ctggactcat tggaagtgtc
     2881 cttatcagcc acggggcgcg gtggcggtgg cagcgctggc gggggggcct cctccagctt
     2941 gccattgcgc agctcctccc gggcggcctc cccgggcggg ggcttcttga cgctctgctt
     3001 cattagtggg ctcttgagga aggccagcgt cttggctttc ttctccttcg ggccctcggg
     3061 ccggtgcttg tggactcggc tgcgactggc cagggagatg tggatgtaca gcaccgtcat
     3121 gatgaccaca ggcaggtaga agccagcaat ggctgtgcca aaggtcactg ctgggttgga
     3181 caggaactgg atgaagcact ggttgtcggg caccgtccgc ttacccacca caaactgcca
     3241 gaacaagatg gcaggcgccc agagcacgaa ggacagtacc caggcagcag caatcatgag
     3301 gcctgccatc ttggtggtgc gccgggcagg gtaggtgaga ggcttggtga cgcagaagta
     3361 gcggtcaaag ctgatgatga gaaggttcat gacggaggcg ttgctcacca cgcagtccag
     3421 ggccagccac aggtcgcaga ccacggcgcc caggggccag tagcccttga tgatgtacac
     3481 ggtgtagagg ttcatggaga aggcgcctat gatgagatca gcacacgcca ggctgaagag
     3541 gaagtagttg ttgactgtct gcagctgcct gttgaccttg atggacagca tcaccaggat
     3601 gttgcccacg acagtcacca ggctcaggga gcctgtcact gtggcaatga agaccatttc
     3661 caccgtctca tagcgattgt gggatgatga cgtgaccagg cgcacggact gattgcccga
     3721 gctgccattg acaggtgtga agttggccat ggtggcaagc ttgcctcgag cagcgctgct
     3781 cgagagatct ataacttcgt ataatgtatg ctatacgaag ttatcctagc gctaccggtc
     3841 gccacctagg tgggtgagga tagcgtgctg atcaccgaga acatgcacat gaaactgtac
     3901 atggagggca ccgtgaacga ccaccacttc aagtgcacat ccgagggcga aggcaagccc
     3961 tacgagggca cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc
     4021 ttcgacatcc tggctaccag cttctagtac ggcagcaaaa cctttatcaa ccacacccag
     4081 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gaggatcacc
     4141 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca gaacggctgc
     4201 ctcatctaca acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag
     4261 aagaaaacac tcggctggga ggccagcacc gagatgctgt accccgctga cagcggcctg
     4321 agaggccata gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccctc
     4381 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cttctacttc
     4441 gtggacagga gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac
     4501 gagatggctg tggccaggta ctgcgacctg cctagcaaac tggggcacag ctgatgcggc
     4561 cgcgacgcta gcgcataact tcgtataaag tatcctatac gaagttatgc ggccgcatcg
     4621 attaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4681 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4741 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4801 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4861 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4921 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4981 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5041 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5101 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5161 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatctgatc
     5221 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     5281 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     5341 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     5401 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     5461 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     5521 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     5581 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     5641 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     5701 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     5761 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     5821 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     5881 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     5941 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6001 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     6061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     6181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     6301 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     6361 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     6421 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     6481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     6601 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6661 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6781 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6841 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6901 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6961 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7021 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7081 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7141 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7201 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7261 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7321 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7381 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7441 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7501 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7561 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7621 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7681 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7741 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7801 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7861 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7921 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7981 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8041 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8101 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8161 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8221 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8281 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8341 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8401 gcggagccta tgga
//
