LOCUS       sequence-387503-       12423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The coding sequence of Triose phosphate isomerase gene was fused 
            downstream Renilla in the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: IRE-TPI-wildtype-TREAT
FEATURES             Location/Qualifiers
     source          1..12423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4086..4110
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4330..5262
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    10221..10355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     10299..10318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(10463..10496)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(10549..10565)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(10581..10603)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    11014..11371
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             11436..11905
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataaag ctttaggcgt gtacggtggg cgcctataaa
     4081 agcagagctc gtttagtgaa ccgtcagatc gcctgcttcc agacgttctt cgccgagagt
     4141 cgtcggggtt tcctgcttca acagtgcttg gacggaaccc ggcgctcgtt ccccaccccg
     4201 gccggccgcc catagccagc cctccgtcac ctcttcaccg caccctcgga ctgccccaag
     4261 gcccccgccg ccgctccagc gccgcgcagc caccgccgcc gccgccgcct ctccttagtc
     4321 gccgccatga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     4381 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     4441 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     4501 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     4561 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     4621 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     4681 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     4741 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     4801 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     4861 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     4921 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     4981 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     5041 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     5101 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     5161 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     5221 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc aggtggtttg tgctccccct
     5281 actgcctata tcgacttcgc ccggcagaag ctagatccca agattgctgt ggctgcgcag
     5341 aactgctaca aagtgactaa tggggctttt actggggaga tcaggtgaga tcgaggtgga
     5401 gaggggtgtg tgggaccctt ccctcacttt cctcgttgag gggaaagcca cagggtgggc
     5461 tccctgctga accttggctt catctcttcc tttagccctg gcatgatcaa agactgcgga
     5521 gccacgtggg tggtcctggg gcactcagag agaaggcatg tctttgggga gtcagatgag
     5581 gttagtagcc aagagagaag ataagggatg tctttttcca agaaggatgt ctcaccaagt
     5641 ctgtttctca acagctgatt gggcagaaag tggcccatgc tctggcagag ggactcggag
     5701 taatcgcctg cattggggag aagctagatg aaagggaagc tggcatcact gagaaggttg
     5761 ttttcgagca gacaaaggtc atcgcaggta tctctggaga aagggacctt tgagcctatc
     5821 cagggccaca gagactcaga gggtagggtc aggccctgga gcctgtcttg gtccccatgc
     5881 tgatccagaa aaggaaaaag gggaggggga gtgacaatct ttgcttgggg cctatgactt
     5941 ctccagcccc aaggtagatg ccacctggaa atcccccaat gtccactagg gggcagtagg
     6001 ccaccgttct tcgtactccg gagaacctgg ctggagagct ctttcttgtt cacccttccc
     6061 tccatctgta tctctgccct gcagataacg tgaaggactg gagcaaggtc gtcctggcct
     6121 atgagcctgt gtgggccatt ggtactggca agactgcaac accccaacag gtaaccgggc
     6181 ccaggagccc tgccctcatc ccagcctgcc tcaataggtt tggacagaca cagcccacat
     6241 ggggcaaccc cttatttcaa agacacagag accttgaacc cagagacagt gacttgtcca
     6301 agggcatcca gtccagggcc tggcttggat cagagccctg gtactctgac tcagtcagaa
     6361 accacactaa gtgtccactg gtgccagtga tttttcctct tagagaggca gaaaaggtct
     6421 tacttaggcc agcttcttgt tctaggccca ggaagtacac gagaagctcc gaggatggct
     6481 gaagtccaac gtctctgatg cggtggctca gagcacccgt atcatttatg gaggtgagtg
     6541 gctttggttc ccggctgagg tggagtgggc tgaggactag actgagccct cggacatgga
     6601 ggtggggatg gggcagactc atcccattct tgaccaagcc cttgttctgc tcccttccca
     6661 ggctctgtga ctggggcaac ctgcaaggag ctggccagcc agcctgatgt ggatggcttc
     6721 cttgtgggtg gtgcttccct caagcccgaa ttcgtggaca tcatcaatgc caaacaatga
     6781 gccccatcca tcttccctac ccttcctgcc aagccaggga ccagatccta cctgcaaagc
     6841 accagaggat atggcctcgg tgcctcagca tcttcagatg tgctgtcact gtccaatctt
     6901 caagccagca gagcatatgg gctcgctggc tccactgtca ttacagtgga cctgatcttc
     6961 tccaatcaaa ggagcagagg atatggcctc gctccctccg actacatcag gctggaagtg
     7021 acatccatca ttcatcccag cagagcatat gggctcgctg ggaccacagc atgcctcgaa
     7081 gtgctgagag actatcatca aaccgacaga ggatatggcc tcgtcggctc ctactccctc
     7141 ctggtggaat acttctccac tcttcaaccc agcagagcat atgggctcgc tgggtccatc
     7201 agagatacaa gatctctcga cagatcctac ctgcaaagca ccagaggata tggcctcggt
     7261 gcctcagcat cttcagatgt gctgtcactg tccaatcttc aagccagcag agcatatggg
     7321 ctcgctggct ccactgtcat tacagtggac ctgatcttct ccaatcaaag gagcagagga
     7381 tatggcctcg ctccctccga ctacatcagg ctggaagtga catccatcat tcatcccagc
     7441 agagcatatg ggctcgctgg gaccacagca tgcctcgaag tgctgagaga ctatcatcaa
     7501 accgacagag gatatggcct cgtcggctcc tactccctcc tggtggaata cttctccact
     7561 cttcaaccca gcagagcata tgggctcgct gggtccatca gagatacaag atctctcgac
     7621 agatcctacc tgcaaagcac cagaggatat ggcctcggtg cctcagcatc ttcagatgtg
     7681 ctgtcactgt ccaatcttca agccagcaga gcatatgggc tcgctggctc cactgtcatt
     7741 acagtggacc tgatcttctc caatcaaagg agcagaggat atggcctcgc tccctccgac
     7801 tacatcaggc tggaagtgac atccatcatt catcccagca gagcatatgg gctcgctggg
     7861 accacagcat gcctcgaagt gctgagagac tatcatcaaa ccgacagagg atatggcctc
     7921 gtcggctcct actccctcct ggtggaatac ttctccactc ttcaacccag cagagcatat
     7981 gggctcgctg ggtccatcag agatacaaga tctctcgaca gatcctacct gcaaagcacc
     8041 agaggatatg gcctcggtgc ctcagcatct tcagatgtgc tgtcactgtc caatcttcaa
     8101 gccagcagag catatgggct cgctggctcc actgtcatta cagtggacct gatcttctcc
     8161 aatcaaagga gcagaggata tggcctcgct ccctccgact acatcaggct ggaagtgaca
     8221 tccatcattc atcccagcag agcatatggg ctcgctggga ccacagcatg cctcgaagtg
     8281 ctgagagact atcatcaaac cgacagagga tatggcctcg tcggctccta ctccctcctg
     8341 gtggaatact tctccactct tcaacccagc agagcatatg ggctcgctgg gtccatcaga
     8401 gatacaagat ctctcgagcc gcggtgcgta agtctgttaa cagaatgctg catacgctga
     8461 gtacttcgaa atgtcatcct ctgtctgaca ctgaacgtaa tccagacgcg taagtcaggc
     8521 cggaaaattc ccgccaccgg aagttgagta gacggtgctg cctgcgactc aaccccagga
     8581 ggactgggtg aacaaagctg cgaagtgatc catgtaagcc ctcagaaccg tctcggaaag
     8641 aggaccccac atgttgtagc ttcaaggccc aatgtcagac cacgccatgg cgtgccactc
     8701 tgcggagagt gcagtctgcg acagtgcccc aggaggactg ggtgaggatc ctacctacaa
     8761 acggcacgag catcagccgt gccgcccagc atcttcatca ctgtccaatc ttcaagcgtg
     8821 cacgatcacg gcacgctcca ctgtcattac agtcttatcc aatcaaactc cagcagcatc
     8881 agcgctggag ctccgactac atcaggacat ccatcattca acgctcgacg atcacgcgag
     8941 cgtccacagc atgcctcaga cactatcatc aaacggctgc acgatcacgg cagccgctcc
     9001 tactgcctct acgttatcga ctcttctgcg acgagcatca gccgtcgcaa catcggacat
     9061 accagatcct acctacaaac ggcacgagca tcagccgtgc cgcccagcat cttcatcact
     9121 gtccaatctt caagcgtgca cgatcacggc acgctccact gtcattacag tcttatccaa
     9181 tcaaactcca gcagcatcag cgctggagct ccgactacat caggacatcc atcattcaac
     9241 gctcgacgat cacgcgagcg tccacagcat gcctcagaca ctatcatcaa acggctgcac
     9301 gatcacggca gccgctccta ctgcctctac gttatcgact cttctgcgac gagcatcagc
     9361 cgtcgcaaca tcggacatac cagatcctac ctacaaacgg cacgagcatc agccgtgccg
     9421 cccagcatct tcatcactgt ccaatcttca agcgtgcacg atcacggcac gctccactgt
     9481 cattacagtc ttatccaatc aaactccagc agcatcagcg ctggagctcc gactacatca
     9541 ggacatccat cattcaacgc tcgacgatca cgcgagcgtc cacagcatgc ctcagacact
     9601 atcatcaaac ggctgcacga tcacggcagc cgctcctact gcctctacgt tatcgactct
     9661 tctgcgacga gcatcagccg tcgcaacatc ggacatacca gatcctacct acaaacggca
     9721 cgagcatcag ccgtgccgcc cagcatcttc atcactgtcc aatcttcaag cgtgcacgat
     9781 cacggcacgc tccactgtca ttacagtctt atccaatcaa actccagcag catcagcgct
     9841 ggagctccga ctacatcagg acatccatca ttcaacgctc gacgatcacg cgagcgtcca
     9901 cagcatgcct cagacactat catcaaacgg ctgcacgatc acggcagccg ctcctactgc
     9961 ctctacgtta tcgactcttc tgcgacgagc atcagccgtc gcaacatcgg acataccaga
    10021 tcttcaatcg atttcgcgcg ggatccagac cacctcccct gcgagctaag ctggacagcc
    10081 aatgacgggt aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta
    10141 ctgcctaatc caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc
    10201 gcagcttccg agggatttga gatccagaca tgataagata cattgatgag tttggacaaa
    10261 ccaaaactag aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct
    10321 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
    10381 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
    10441 gtggtatggc tgattatgat ctgaagttcc tatactattt gaagaatagg aacttcggaa
    10501 taggaacttc ggatctcata tggccatatg ggggcgccta gagaaggagt gagggctgga
    10561 taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat
    10621 ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca
    10681 aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca
    10741 ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc
    10801 ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc
    10861 ttgacatcta ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc
    10921 aaacttaagg gagtggtaac aggctagccc gggcccatat tttcagacaa atacagaaac
    10981 acagtcagac agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt
    11041 cggtcccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct
    11101 cctaccagaa ccacatatcc ctcctctaag gggggtgcac caaagagtcc taaacgatcg
    11161 ggattttagg actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc
    11221 agggttgcgg ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag
    11281 ctagttagct aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc
    11341 ggacagacac agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg
    11401 ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt
    11461 caatcactca gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca
    11521 agagggttta ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgcgc
    11581 cgagtgaggg gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag
    11641 cgagaagcga actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg
    11701 caccctggaa acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga
    11761 ccatctgttc ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc
    11821 ccatattcag ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca
    11881 gatatcctgt ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttt
    11941 tctattctca gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg
    12001 ccaaacctac aggtggggtc tttcacatgt atatgtcaaa aataaaaatc aactaattga
    12061 ctagtaatta atatgactgg cataatggga aattgatcct gacagatgca aactggcttc
    12121 tcagcagcgc atttatgttg tcaactgagg aaggaaacgt taatgacaga aactctaagt
    12181 aatttccacg tttatctatt tttatttata ctagctttgg taacaggaat attgcagcat
    12241 tcatgcacat tgaaaccctt atgaaataaa aacatctgtg catttaaaat ggaattaaca
    12301 ttttaaatgt taaaaaaagc tggcttagct tccccccgcc ccctagggca tagaacaagt
    12361 caaatgcttt atatatttga gtttgggatg tattaggaaa ctcctaagag caaagctagc
    12421 ttg
//
