LOCUS       sequence-387510-       13186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SunTag cassette was added in the coding region in the let7-IRE-TREAT
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: IRE-let7-SunTag-TREAT
FEATURES             Location/Qualifiers
     source          1..13186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3706..3753)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3872..3890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3908..3926
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3944..3962
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3980..3998
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4016..4034
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4079..4103
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4362..4418
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4434..4490
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4506..4562
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4578..4634
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4650..4706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4722..4778
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4794..4850
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4866..4922
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4938..4994
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5010..5066
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5082..5138
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5154..5210
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5226..5282
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5298..5354
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5370..5426
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5442..5498
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5514..5570
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5586..5642
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5658..5714
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5730..5786
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5802..5858
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5874..5930
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6018..6074
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6108..7037
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7038..7040,7041..7361)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7041..7364
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    10989..11123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11067..11086
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(11231..11264)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(11317..11333)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(11349..11371)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    11777..12134
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             12199..12668
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaaw cacacacaca cacayrcaca cacacacaca
     2821 cacacacaca cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca
     2881 atcacacaca cacaatcaca cacacacaca tcaatcaatc aatcaatcaa tataccaaaa
     2941 atgatagctc atacctgtaa tcttagatgt gggaaggcta aaacagcagg ctacccatga
     3001 cttcaaggtc accctgggtt acagagtagc taatagtcta gcttgaacta tagtgtagga
     3061 tctttaaaaa aaaattaacc aaccaaccaa caaacaaaca aacaaacaac aacaacaaca
     3121 aatggaagac agaggacaaa aaaaaataaa agaaaaagta gaaagccaat ggagatacta
     3181 tcttaaaatt agaaatattt atgacacatt ctgaatactc catgtttatt tcaaggaagc
     3241 caatgtaact ttaagtacta tggccattta atgtaaatac ttaagaaaaa aaaccaaatt
     3301 aattttgata catgctgcat gtgaaagacc cccgctgacg ggtagtcaat cactcagagg
     3361 agaccctctc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
     3421 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag tgaggggttg
     3481 tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct tgccaaacct
     3541 acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct tttattttat
     3601 ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa ctagagcctg
     3661 gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt tcctatactt
     3721 tctagagaat aggaacttcg gaataggaac ttcgcggcca gatcttttgc ggccgctttg
     3781 ggccggccga attctttact ccctatcagt gatagagaac gtatgaagag tttactccct
     3841 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3901 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3961 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     4021 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     4081 ctcgtttagt gaaccgtcag atcgcctgct tccagacgtt cttcgccgag agtcgtcggg
     4141 gtttcctgct tcaacagtgc ttggacggaa cccggcgctc gttccccacc ccggccggcc
     4201 gcccatagcc agccctccgt cacctcttca ccgcaccctc ggactgcccc aaggcccccg
     4261 ccgccgctcc agcgccgcgc agccaccgcc gccgccgccg cctctcctta gtcgccgcca
     4321 tgaccggtcc gggtggatct ggaggtggag gttctggagg agaagaactt ttgagcaaga
     4381 attatcatct tgagaacgaa gtggctcgtc ttaagaaagg ttctggcagt ggagaagaac
     4441 tgctttcaaa gaattaccac ctggaaaatg aggtagctag actgaaaaag gggagcggaa
     4501 gtggggagga gttgctgagc aaaaattatc atttggagaa cgaagtagca cgactaaaga
     4561 aagggtccgg atcgggtgag gagttactct cgaaaaatta tcatctcgaa aacgaagtgg
     4621 ctcggctaaa aaagggcagt ggttctggag aagagctatt atctaaaaac taccacctcg
     4681 aaaatgaggt ggcacgctta aaaaagggaa gtggcagtgg tgaagagcta ctatccaaga
     4741 attatcatct tgagaacgag gtagcgcgtt tgaagaaggg ttccggctca ggagaggaac
     4801 tgctctcgaa gaactatcat cttgaaaatg aggtcgctcg attaaaaaag ggatcgggca
     4861 gtggtgagga actactttca aagaattacc acctcgaaaa cgaagtagct cgattaaaga
     4921 aaggttcagg gtcgggtgaa gaattactga gtaaaaatta tcatctggaa aatgaggtag
     4981 cgagactaaa aaaggggagt ggttctggcg aagagttgct atcgaaaaat tatcatcttg
     5041 agaacgaagt tgctaggctc aaaaagggct caggctcagg cgaggagttg ctctcgaaaa
     5101 actaccactt ggaaaatgag gtcgcgaggt tgaaaaaggg gagcgggtcg ggcgaggagt
     5161 tattgagcaa aaactatcat ttagagaacg aagtcgcgcg cttaaagaaa ggctcgggct
     5221 cgggcgaaga actcttatcg aagaactacc acctcgaaaa tgaggtcgcc aggttgaaaa
     5281 agggcagtgg cagcggggag gaactcttga gcaagaacta ccacttggag aatgaggtcg
     5341 cgagattgaa gaaagggtcg gggagcggcg aggaattgct cagcaagaat tatcatttgg
     5401 agaacgaagt cgccaggctc aagaaaggct cggggtcggg ggaggaattg ttgagtaaaa
     5461 actaccactt ggaaaatgaa gtcgccaggc tcaaaaaagg gagtgggagc ggcgaagagt
     5521 tattgagcaa aaattaccac ttggagaacg aagtggcaag gctcaagaaa gggagcggca
     5581 gcggggagga gctcttatcg aagaactacc acttagagaa tgaagtcgcc cgcttgaaga
     5641 aaggctcggg gagcggggaa gagctcttga gcaagaacta ccacttggaa aatgaggtgg
     5701 cgcgcttgaa gaaagggagc gggagcgggg aagagttact atctaagaat tatcatctcg
     5761 agaacgaggt ggctcgacta aagaagggct ccggcagtgg ggaggaactc ctgtcgaaga
     5821 actatcatct tgaaaatgag gttgcaagac ttaaaaaggg gtccggatca ggtgaggaac
     5881 tactcagtaa gaattaccac ctggaaaacg aagtagctcg attaaagaaa ggttcagggt
     5941 cgggtgaaga actgctctca aaagattatc atttggaaaa tgaggttgca cgtttaaaaa
     6001 agggaagtgg cagtggtgag gaacttctgt cgaaaaatta tcatctcgag aatgaagtag
     6061 cccgacttaa aaagggttct ggctcgggtc agcggccgca ccggtcagct tccaaggtgt
     6121 acgaccccga gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc
     6181 aaatgaacgt gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg
     6241 ccgtgatttt tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc
     6301 acatcgagcc cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca
     6361 agagcgggaa tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg
     6421 agctgctgaa ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg
     6481 cctttcacta ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg
     6541 tcgtggacgt gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga
     6601 tcaagagcga agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc
     6661 tcccaagcaa gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat
     6721 tcaaggagaa gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg
     6781 ttaagggagg caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg
     6841 ccagcgacga tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta
     6901 ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact
     6961 tcagccagga ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg
     7021 tgctgaagaa cgagcagatg ggagtgcagg tggaaaccat ctccccagga gacgggcgca
     7081 ccttccccaa gcgcggccag acctgtgtgg tgcactacac cgggatgctt gaagatggaa
     7141 agaaagtcga ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg
     7201 aggtgatccg aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac
     7261 tgactatatc tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac
     7321 atgccactct cgtcttcgat gtggagcttc taaaaccgga ataagggcca gggaagaact
     7381 ggctcggaaa gaggacccca catgttgtag cttcaagttt gggccaggga agaactgatc
     7441 gccgtgtaac cgcggggaca gcctattgaa ctacctcact cggagcacag cctattgaac
     7501 tacctcaggc ctgcacagcc tattgaacta cctcaccgcg gcgcgcgcgg gatccggatc
     7561 caacctacaa agaacgagca gtaagtcgac agatcctacc tgcaaagcac cagaggatat
     7621 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     7681 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     7741 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     7801 catcccagca gagcatatgg gctcgctggg accacagcat gcctcgaagt gctgagagac
     7861 tatcatcaaa ccgacagagg atatggcctc gtcggctcct actccctcct ggtggaatac
     7921 ttctccactc ttcaacccag cagagcatat gggctcgctg ggtccatcag agatacaaga
     7981 tctctcgaca gatcctacct gcaaagcacc agaggatatg gcctcggtgc ctcagcatct
     8041 tcagatgtgc tgtcactgtc caatcttcaa gccagcagag catatgggct cgctggctcc
     8101 actgtcatta cagtggacct gatcttctcc aatcaaagga gcagaggata tggcctcgct
     8161 ccctccgact acatcaggct ggaagtgaca tccatcattc atcccagcag agcatatggg
     8221 ctcgctggga ccacagcatg cctcgaagtg ctgagagact atcatcaaac cgacagagga
     8281 tatggcctcg tcggctccta ctccctcctg gtggaatact tctccactct tcaacccagc
     8341 agagcatatg ggctcgctgg gtccatcaga gatacaagat ctctcgacag atcctacctg
     8401 caaagcacca gaggatatgg cctcggtgcc tcagcatctt cagatgtgct gtcactgtcc
     8461 aatcttcaag ccagcagagc atatgggctc gctggctcca ctgtcattac agtggacctg
     8521 atcttctcca atcaaaggag cagaggatat ggcctcgctc cctccgacta catcaggctg
     8581 gaagtgacat ccatcattca tcccagcaga gcatatgggc tcgctgggac cacagcatgc
     8641 ctcgaagtgc tgagagacta tcatcaaacc gacagaggat atggcctcgt cggctcctac
     8701 tccctcctgg tggaatactt ctccactctt caacccagca gagcatatgg gctcgctggg
     8761 tccatcagag atacaagatc tctcgacaga tcctacctgc aaagcaccag aggatatggc
     8821 ctcggtgcct cagcatcttc agatgtgctg tcactgtcca atcttcaagc cagcagagca
     8881 tatgggctcg ctggctccac tgtcattaca gtggacctga tcttctccaa tcaaaggagc
     8941 agaggatatg gcctcgctcc ctccgactac atcaggctgg aagtgacatc catcattcat
     9001 cccagcagag catatgggct cgctgggacc acagcatgcc tcgaagtgct gagagactat
     9061 catcaaaccg acagaggata tggcctcgtc ggctcctact ccctcctggt ggaatacttc
     9121 tccactcttc aacccagcag agcatatggg ctcgctgggt ccatcagaga tacaagatct
     9181 ctcgagccgc ggtgcgtaag tctgttaaca gaatgctgca tacgctgagt acttcgaaat
     9241 gtcatcctct gtctgacact gaacgtaatc cagacgcgta agtcaggccg gaaaattccc
     9301 gccaccggaa gttgagtaga cggtgctgcc tgcgactcaa ccccaggagg actgggtgaa
     9361 caaagctgcg aagtgatcca tgtaagccct cagaaccgtc tcggaaagag gaccccacat
     9421 gttgtagctt caaggcccaa tgtcagacca cgccatggcg tgccactctg cggagagtgc
     9481 agtctgcgac agtgccccag gaggactggg tgaggatcct acctacaaac ggcacgagca
     9541 tcagccgtgc cgcccagcat cttcatcact gtccaatctt caagcgtgca cgatcacggc
     9601 acgctccact gtcattacag tcttatccaa tcaaactcca gcagcatcag cgctggagct
     9661 ccgactacat caggacatcc atcattcaac gctcgacgat cacgcgagcg tccacagcat
     9721 gcctcagaca ctatcatcaa acggctgcac gatcacggca gccgctccta ctgcctctac
     9781 gttatcgact cttctgcgac gagcatcagc cgtcgcaaca tcggacatac cagatcctac
     9841 ctacaaacgg cacgagcatc agccgtgccg cccagcatct tcatcactgt ccaatcttca
     9901 agcgtgcacg atcacggcac gctccactgt cattacagtc ttatccaatc aaactccagc
     9961 agcatcagcg ctggagctcc gactacatca ggacatccat cattcaacgc tcgacgatca
    10021 cgcgagcgtc cacagcatgc ctcagacact atcatcaaac ggctgcacga tcacggcagc
    10081 cgctcctact gcctctacgt tatcgactct tctgcgacga gcatcagccg tcgcaacatc
    10141 ggacatacca gatcctacct acaaacggca cgagcatcag ccgtgccgcc cagcatcttc
    10201 atcactgtcc aatcttcaag cgtgcacgat cacggcacgc tccactgtca ttacagtctt
    10261 atccaatcaa actccagcag catcagcgct ggagctccga ctacatcagg acatccatca
    10321 ttcaacgctc gacgatcacg cgagcgtcca cagcatgcct cagacactat catcaaacgg
    10381 ctgcacgatc acggcagccg ctcctactgc ctctacgtta tcgactcttc tgcgacgagc
    10441 atcagccgtc gcaacatcgg acataccaga tcctacctac aaacggcacg agcatcagcc
    10501 gtgccgccca gcatcttcat cactgtccaa tcttcaagcg tgcacgatca cggcacgctc
    10561 cactgtcatt acagtcttat ccaatcaaac tccagcagca tcagcgctgg agctccgact
    10621 acatcaggac atccatcatt caacgctcga cgatcacgcg agcgtccaca gcatgcctca
    10681 gacactatca tcaaacggct gcacgatcac ggcagccgct cctactgcct ctacgttatc
    10741 gactcttctg cgacgagcat cagccgtcgc aacatcggac ataccagatc ttcaatcgat
    10801 ttcgcgcggg atccagacca cctcccctgc gagctaagct ggacagccaa tgacgggtaa
    10861 gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact gcctaatcca
    10921 aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc agcttccgag
    10981 ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa
    11041 tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca
    11101 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
    11161 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    11221 attatgatct gaagttccta tactatttga agaataggaa cttcggaata ggaacttcgg
    11281 atctcatatg gccatatggg ggcgcctaga gaaggagtga gggctggata aagggaggat
    11341 cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga
    11401 ggcggaggct tagggaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
    11461 ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
    11521 cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa
    11581 ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg
    11641 gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt
    11701 aacaggctag cccgggccca tattttcaga caaatacaga aacacagtca gacagagaca
    11761 acacagaacg atgctgcagc agacaagacg cgcggcgcgg cttcggtccc aaaccgaaag
    11821 caaaaattca gacggaggcg ggaactgttt taggttctcg tctcctacca gaaccacata
    11881 tccctcctct aaggggggtg caccaaagag tcctaaacga tcgggatttt aggactcagg
    11941 tcgggccaca aaaacggccc ccgaagtccc tgggacgtct cccagggttg cggccgggtg
    12001 ttccgaactc gtcagttcca ccacgggtcc gccagataca gagctagtta gctaactagt
    12061 accgacgcag gcgcataaaa tcagtcatag acactagaca atcggacaga cacagataag
    12121 ttgctggcca gcttacctcc cggtggtggg tcggtggtcc ctgggcaggg gtctcccgat
    12181 cccggacgag cccccaaatg aaagaccccc gctgacgggt agtcaatcac tcagaggaga
    12241 ccctcccaag gaacagcgag accacaagtc ggatgcaact gcaagagggt ttattggata
    12301 cacgggtacc cgggcgactc agtcaatcgg aggactggcg cgccgagtga ggggttgtgg
    12361 gctcttttat tgagctcggg gagcagaagc gcgcgaacag aagcgagaag cgaactgatt
    12421 ggttagttca aataaggcac agggtcattt caggtccttg gggcaccctg gaaacatctg
    12481 atggttctct agaaactgct gagggctgga ccgcatctgg ggaccatctg ttcttggccc
    12541 tgagccgggg caggaactgc ttaccacaga tatcctgttt ggcccatatt cagctgttcc
    12601 atctgttctt ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc
    12661 atattcagct gttccatctg ttcctgacct tgatctgaac ttttctattc tcagttatgt
    12721 atttttccat gccttgcaaa atggcgttac ttaagctagc ttgccaaacc tacaggtggg
    12781 gtctttcaca tgtatatgtc aaaaataaaa atcaactaat tgactagtaa ttaatatgac
    12841 tggcataatg ggaaattgat cctgacagat gcaaactggc ttctcagcag cgcatttatg
    12901 ttgtcaactg aggaaggaaa cgttaatgac agaaactcta agtaatttcc acgtttatct
    12961 atttttattt atactagctt tggtaacagg aatattgcag cattcatgca cattgaaacc
    13021 cttatgaaat aaaaacatct gtgcatttaa aatggaatta acattttaaa tgttaaaaaa
    13081 agctggctta gcttcccccc gccccctagg gcatagaaca agtcaaatgc tttatatatt
    13141 tgagtttggg atgtattagg aaactcctaa gagcaaagct agcttg
//
