LOCUS       sequence-387514-       11243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Let7-miRNA binding sites (3x) were integrated in between the stop 
            codon and MS2 stem-loops in the SunTag reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: Let7-SunTag
FEATURES             Location/Qualifiers
     source          1..11243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1147..1938,1939..2007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1770..1789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2008..2112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2180..2198
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2236..2258)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2358..2377
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2571..2593
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2585..2602
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2586..2602
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3686..3733)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3780..3798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3816..3834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3852..3870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3888..3906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3924..3942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3960..3978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3996..4014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4059..4083
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4160..4292
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4275..4294
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4337..4355
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4359..4368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4434..4490
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4506..4562
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4578..4634
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4650..4706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4722..4778
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4794..4850
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4866..4922
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4938..4994
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5010..5066
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5082..5138
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5154..5210
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5226..5282
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5298..5354
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5370..5426
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5442..5498
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5514..5570
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5586..5642
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5658..5714
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5730..5786
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5802..5858
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5874..5930
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5946..6002
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6090..6146
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6180..7109
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(7110..7112,7113..7433)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             7113..7436
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    9041..9175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9119..9138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9283..9316)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9369..9385)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9401..9423)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9834..10191
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10256..10725
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttgctaaagt atatatgagt aaacttggtc
     1141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1261 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2101 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2161 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2221 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2281 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2341 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2401 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     2461 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2521 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2581 acgttgtaaa acgacggcca gtgaattaat tccaagctaa gtgaaagact ctgtctcaaa
     2641 aaccaacctg acacctgagc cggagcgttg cctctcacta ccataaatat atatacacaa
     2701 cacacacaca cacacacaca cacacacaca cacacacaca cacacaatca tacacataaa
     2761 cacaatcaca cacacacaca tgcacaatca cacacacaca atcacacaca aacatacata
     2821 cacacaatca cacacaacca tacacacata tacacaatca cacacacaaa cacaatcaca
     2881 cacacacaca tcaatcaatc aatcaatcaa tataccaaaa atgatagctc atacctgtaa
     2941 tcttagatgt gggaaggcta aaacagcagg ctacccatga cttcaaggtc accctgggtt
     3001 acagagtagc taatagtcta gcttgaacta tagtgtagga tctttaaaaa aaaattaacc
     3061 aaccaaccaa caaacaaaca aacaaacaac aacaacaaca aatggaagac agaggacaaa
     3121 aaaaaataaa agaaaaagta gaaagccaat ggagatacta tcttaaaatt agaaatattt
     3181 atgrcacatt ctgaatactc catgtttatt tcaaggaagc caatgtaact ttaagtacta
     3241 tggccattta atgtaaatac ttaagaaaaa aaaccaaatt aattttgata catgctgcat
     3301 gtgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctctc aaggaacagc
     3361 gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga
     3421 ctcagtcaat cggaggactg gcgccccgag tgaggggttg tgggctcgag ctcggggagc
     3481 agaagcgcgc gaacagaagt cgagctagct tgccaaacct acaggtgggg tctttcattc
     3541 cccccttttt ctggagacta aataaaatct tttattttat ctatggctcg tactctatag
     3601 gcttcagctg gtgatattgt tgagtcaaaa ctagagcctg gaccactgat atcctgtctt
     3661 taacaaattg gactaatccg gatctgaagt tcctatactt tctagagaat aggaacttcg
     3721 gaataggaac ttcgcggcca gatcttttgc ggccgctttg ggccggccga attctttact
     3781 ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat
     3841 gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga
     3901 tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact
     3961 ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat
     4021 aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag
     4081 atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta ccacttccta
     4141 ccctcgtaaa accggtcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     4201 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     4261 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     4321 ttaaggctag agtacttaat acgactcact ataggctagc caccatgggc gccatcatac
     4381 acgcggccgc aatcatcggt ccgggtggat ctggaggtgg aggttctgga ggagaagaac
     4441 ttttgagcaa gaattatcat cttgagaacg aagtggctcg tcttaagaaa ggttctggca
     4501 gtggagaaga actgctttca aagaattacc acctggaaaa tgaggtagct agactgaaaa
     4561 aggggagcgg aagtggggag gagttgctga gcaaaaatta tcatttggag aacgaagtag
     4621 cacgactaaa gaaagggtcc ggatcgggtg aggagttact ctcgaaaaat tatcatctcg
     4681 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg agaagagcta ttatctaaaa
     4741 actaccacct cgaaaatgag gtggcacgct taaaaaaggg aagtggcagt ggtgaagagc
     4801 tactatccaa gaattatcat cttgagaacg aggtagcgcg tttgaagaag ggttccggct
     4861 caggagagga actgctctcg aagaactatc atcttgaaaa tgaggtcgct cgattaaaaa
     4921 agggatcggg cagtggtgag gaactacttt caaagaatta ccacctcgaa aacgaagtag
     4981 ctcgattaaa gaaaggttca gggtcgggtg aagaattact gagtaaaaat tatcatctgg
     5041 aaaatgaggt agcgagacta aaaaagggga gtggttctgg cgaagagttg ctatcgaaaa
     5101 attatcatct tgagaacgaa gttgctaggc tcaaaaaggg ctcaggctca ggcgaggagt
     5161 tgctctcgaa aaactaccac ttggaaaatg aggtcgcgag gttgaaaaag gggagcgggt
     5221 cgggcgagga gttattgagc aaaaactatc atttagagaa cgaagtcgcg cgcttaaaga
     5281 aaggctcggg ctcgggcgaa gaactcttat cgaagaacta ccacctcgaa aatgaggtcg
     5341 ccaggttgaa aaagggcagt ggcagcgggg aggaactctt gagcaagaac taccacttgg
     5401 agaatgaggt cgcgagattg aagaaagggt cggggagcgg cgaggaattg ctcagcaaga
     5461 attatcattt ggagaacgaa gtcgccaggc tcaagaaagg ctcggggtcg ggggaggaat
     5521 tgttgagtaa aaactaccac ttggaaaatg aagtcgccag gctcaaaaaa gggagtggga
     5581 gcggcgaaga gttattgagc aaaaattacc acttggagaa cgaagtggca aggctcaaga
     5641 aagggagcgg cagcggggag gagctcttat cgaagaacta ccacttagag aatgaagtcg
     5701 cccgcttgaa gaaaggctcg gggagcgggg aagagctctt gagcaagaac taccacttgg
     5761 aaaatgaggt ggcgcgcttg aagaaaggga gcgggagcgg ggaagagtta ctatctaaga
     5821 attatcatct cgagaacgag gtggctcgac taaagaaggg ctccggcagt ggggaggaac
     5881 tcctgtcgaa gaactatcat cttgaaaatg aggttgcaag acttaaaaag gggtccggat
     5941 caggtgagga actactcagt aagaattacc acctggaaaa cgaagtagct cgattaaaga
     6001 aaggttcagg gtcgggtgaa gaactgctct caaaagatta tcatttggaa aatgaggttg
     6061 cacgtttaaa aaagggaagt ggcagtggtg aggaacttct gtcgaaaaat tatcatctcg
     6121 agaatgaagt agcccgactt aaaaagggtt ctggctcggg tcagcggccg caccggtcag
     6181 cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg
     6241 ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc
     6301 acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc
     6361 acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg
     6421 gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca
     6481 ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg
     6541 gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc
     6601 atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg
     6661 atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg
     6721 tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct
     6781 acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg
     6841 agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg
     6901 cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct
     6961 tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga
     7021 agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct
     7081 tcgtggagcg cgtgctgaag aacgagcaga tgggagtgca ggtggaaacc atctccccag
     7141 gagacgggcg caccttcccc aagcgcggcc agacctgtgt ggtgcactac accgggatgc
     7201 ttgaagatgg aaagaaagtc gattcctccc gggacagaaa caagcccttt aagtttatgc
     7261 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
     7321 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
     7381 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaaccg gaataagggc
     7441 cagggaagaa ctgatcgccg tgtaaccgcg gggacagcct attgaactac ctcactcgga
     7501 gcacagccta ttgaactacc tcaggcctgc acagcctatt gaactacctc accgcggcgc
     7561 gcgcgggatc cggatccaac ctacaaacgg gtggaggatc accccacccg acacttcaca
     7621 atcaaggggt acaatacaca agggtggagg aacaccccac cctccagaca cattacacag
     7681 aaatccaatc aaacagaagc accatcaggg cttctgctac caaatttatc tcaaaaaact
     7741 acaacaagga atcaccatca gggattccct gtgcaatata cgtcaaacga gggccacgac
     7801 gggaggacga tcacgcctcc cgaatatcgg catgtctggc tttcgaattc agtgcgtgga
     7861 gcatcagccc acgcagccaa tcagagtcga atacaagtcg actttcgcga agagcatcag
     7921 ccttcgcgcc attcttacac aaaccacact ctcccctaca ggaacagcat cagcgttcct
     7981 gcccagtacc caactcaaga aaatttatgt ccccatgcag catcagcgca tgggccccaa
     8041 gaatacatcc ccaacaaaat cacatccgag caccaacagg gctcggagtg ttgtttcttg
     8101 tccaactgga caaaccctcc atggaccatc aggccatgga ctctcaccaa caagacaaaa
     8161 actactcttc tcgaagcagc atcagcgctt cgaaacactc gagcatacat tgtgcctatt
     8221 tcttgggtgg acgatcacgc cacccatgct ctcacgaatt tcaaaacacg gacaaggacg
     8281 agcaccacca gggctcgtcg ttccacgtcc aatacgatta cttacctttc gggatcacga
     8341 tcacggatcc cgcagctaca tcacttccac tcaggacatt caagcatgca cgatcacggc
     8401 atgctccaca agtctcaacc acagaaacta ccaaatgggt tcagcaccag cgaacccact
     8461 cctacctcaa acctcttccc acaaaactgg caagcaggat caccgcttgc ccattccaac
     8521 ataccaaatc aaaaacaatt actggtacag catcagcgta ccagcccaca tctctcacta
     8581 ctatcaaaaa ccaaaccgtt cagcaacagc gaacggtaca cacggaaaaa tcaactggtt
     8641 tacaaatacg aaagacgatc acgctttcgt ccagcgcaaa ctattacgaa aaacatccga
     8701 cgggaagagc aacagccttc ccgcggcgga aaacctcaca aaaacacgac aaacggatgc
     8761 acgaacacgg catccgccga caacccacaa acttacaacc aggcaaacgg tgcaggatca
     8821 ccgcaccgta catcaaacac ctcagatctc atttcgcgcg ggatccagac cacctcccct
     8881 gcgagctaag ctggacagcc aatgacgggt aagagagtga catttttcac taacctaaga
     8941 caggagggcc gtcagagcta ctgcctaatc caaagacggg taaaagtgat aaaaatgtat
     9001 cactccaacc taagacaggc gcagcttccg agggatttga gatccagaca tgataagata
     9061 cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc ttatttgtga
     9121 aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac aagttaacaa
     9181 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     9241 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctgaagttcc tatactattt
     9301 gaagaatagg aacttcggaa taggaacttc ggatctcata tggccatatg ggggcgccta
     9361 gagaaggagt gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa
     9421 gggggagaga cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct
     9481 tgacccaggg aggggggtca aaagccaagg cttcccaggt cacgatgtag gggacctggt
     9541 ctgggtgtcc atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc
     9601 ggttaaaggt gccgtctcgc ggccatccga cgttaaaggt tggccattct gcagagcaga
     9661 aggtaaccca acgtctcttc ttgacatcta ccgactggtt gtgagcgatc cgctcgacat
     9721 ctttccagtg acctaaggtc aaacttaagg gagtggtaac aggctagccc gggcccatat
     9781 tttcagacaa atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcaga
     9841 caagacgcgc ggcgcggctt cggtcccaaa ccgaaagcaa aaattcagac ggaggcggga
     9901 actgttttag gttctcgtct cctaccagaa ccacatatcc ctcctctaag gggggkgcac
     9961 caaagagtcc taaacgatcg ggattttagg actcaggtcg ggccacaaaa acggcccccg
    10021 aagtccctgg gacgtctccc agggttgcgg ccgggtgttc cgaactcgtc agttccacca
    10081 cgggtccgcc agatacagag ctagttagct aactagtacc gacgcaggcg cataaaatca
    10141 gtcatagaca ctagacaatc ggacagacac agataagttg ctggccagct tacctcccgg
    10201 tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa
    10261 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
    10321 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
    10381 caatcggagg actggcgcgc cgagtgaggg gttgtgggct cttttattga gctcggggag
    10441 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
    10501 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag
    10561 ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta
    10621 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc cctgagccgg
    10681 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
    10741 ctgaccttga tctgaacttt tctattctca gttatgtatt tttccatgcc ttgcaaaatg
    10801 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcacatgt atatgtcaaa
    10861 aataaaaatc aactaattga ctagtaatta atatgactgg cataatggga aattgatcct
    10921 gacagatgca aactggcttc tcagcagcgc atttatgttg tcaactgagg aaggaaacgt
    10981 taatgacaga aactctaagt aatttccacg tttatctatt tttatttata ctagctttgg
    11041 taacaggaat attgcagcat tcatgcacat tgaaaccctt atgaaataaa aacatctgtg
    11101 catttaaaat ggaattaaca ttttaaatgt taaaaaaagc tggcttagct tccccccgcc
    11161 ccctagggca tagaacaagt caaatgcttt atatatttga gtttgggatg tattaggaaa
    11221 ctcctaagag caaagctagc ttg
//
