LOCUS       sequence-387517-        7502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Melamed D, Young DL, Miller CR, Fields S
  TITLE     Combining natural sequence variation with high throughput mutational
            data to reveal protein interaction sites.
  JOURNAL   PLoS Genet. 2015 Feb 11;11(2):e1004918. doi: 
            10.1371/journal.pgen.1004918. eCollection 2015 Feb.
  PUBMED    25671604
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-02-11"; volume: "11(2):e1004918. doi"; pages: "
            10.1371/journal.pgen.1004918. eCollection 2015 Feb"
COMMENT     Sequence Label: pOBD2 Pab1 E181R (123-204)
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             536..976
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     terminator      1693..1880
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1748..1772)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    2710..3872
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      3035..3872
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(3711..4385)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4386..4487)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(4507..4525)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4593..4697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4698..4766,4767..5558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4916..4935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5729..6317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6218..6237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6471..6488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(7306..7502,1..508)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
      301 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
      361 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
      421 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
      481 tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgaa
      541 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
      601 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
      661 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
      721 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
      781 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
      841 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
      901 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
      961 acagttgact gtatcgaact atctattcga tgatgaagat accccaccaa acccaaaaaa
     1021 agagatcgaa ttccagctga ccaccatggc aaaaggatcc ggtaacatct ttatcaagaa
     1081 cttgcaccct gatattgaca acaaggcttt gtatgacact ttctctgtgt ttggtgacat
     1141 cttgtccagc aagattgcca ccgacgaaaa cggaaaatcc aagggttttg ggtttgttca
     1201 cttcgaagaa gaaggtgctg ccaagagagc tattgatgct ttgaatggta tgctgttgaa
     1261 cggtcaagaa atttatgttg ctcctcactt gtctagatga gtcgacctgc agagatctat
     1321 gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc
     1381 aatttctttc atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt
     1441 caatcttggc catgtaacct ctgatctata gaatttttta aatgactaga attaatgccc
     1501 atcttttttt tggacctaaa ttcttcatga aaatatatta cgagggctta ttcagaagct
     1561 ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc
     1621 ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac
     1681 acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     1741 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     1801 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt
     1861 attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc
     1921 caattgtaga tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc
     1981 ctcagaggac aacacctgtt gtaatcgttc ttccacacgg atcctggcgt aatagcgaag
     2041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     2101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataatc gctgggccat
     2161 tctcatgaag aatatcttga atttattgtc atattactag ttggtgtgga agtcctaata
     2221 tcggtgatca atatagtggt tgacatgctg gctagtcaac attgagcctt ttgatcatgc
     2281 aaatatatta cggtatttta caatcaaata tcaaacttaa ctattgactt tataacttat
     2341 ttaggtggta acattcttat aaaaaagaaa aaaattactg caaaacagta ctagctttta
     2401 acttgtatcc taggttatct atgctgtctc accatagaga atattaccta tttcagaatg
     2461 tatgtccatg attcgccggg taaatacata taatacacaa atctggctta ataaagtcta
     2521 taatatatct cataaagaag tgctaaattg gctagtgcta tatattttta agaaaatttc
     2581 ttttgactaa gtccatatcg actttgtaaa agttcacatt agcatacata tattacacga
     2641 gccagaaata gtaacttttg cctaaatcac aaattgcaaa atttaattgc ttgcaaaagg
     2701 tcacatgctt ataatcaact tttttaaaaa tttaaaatac ttttttattt tttattttta
     2761 aacataaatg aaataattta tttattgttt atgattaccg aaacataaaa cctgctcaag
     2821 aaaaagaaac tgttttgtcc ttggaaaaaa agcactacct aggagcggcc aaaatgccga
     2881 ggctttcata gcttaaactc tttacagaaa ataggcatta tagatcagtt cgagttttct
     2941 tattcttcct tccggtttta tcgtcacagt tttacagtaa ataagtatca cctcttagag
     3001 ttcgatgata agctgtcaaa catgagaatt aattccacat gttaaaatag tgaaggagca
     3061 tgttcggcac acagtggacc gaacgtgggg taagtgcact agggtccggt taaacggatc
     3121 tcgcattgat gaggcaacgc taattatcaa catatagatt gttatctatc tgcatgaaca
     3181 cgaaatcttt acttgacgac ttgaggctga tggtgtttat gcaaagaaac cactgtgttt
     3241 aatatgtgtc actgtttgat attactgtca gcgtagaaga taatagtaaa agcggttaat
     3301 aagtgtattt gagataagtg tgataaagtt tttacagcga aaagacgata aatacaagaa
     3361 aatgattacg aggatacgga gagaggtatg tacatgtgta tttatatact aagctgccgg
     3421 cggttgtttg caagaccgag aaaaggctag caagaatcgg gtcattgtag cgtatgcgcc
     3481 tgtgaacatt ctcttcaaca agtttgattc cattgcggtg aaatggtaaa agtcaacccc
     3541 ctgcgatgta tattttcctg tacaatcaat caaaaagcca aatgatttag cattatcttt
     3601 acatcttgtt attttacaga ttttatgttt agatctttta tgcttgcttt tcaaaaggct
     3661 tgcaggcaag tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta
     3721 gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct
     3781 ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc
     3841 gtcagtccac cagctaacat aaaatgtaag ctctcggggc tctcttgcct tccaacccag
     3901 tcagaaatcg agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag
     3961 ggaataaacg aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga
     4021 aatacgagtc ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca
     4081 tctccatgca gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc
     4141 ttaggttgat tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat
     4201 gcttttacaa gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg
     4261 ggcacacata taatacccag caagtcagca tcggaatcta gtgcacattc tgcggcctct
     4321 gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca
     4381 gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac
     4441 caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttgaagac
     4501 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4561 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6481 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6541 ttcattaatg caggatccgg gatcgaagaa atgatggtaa atgaaatagg aaatcaagga
     6601 gcatgaaggc aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa agtgttgata
     6661 tgatgtattt ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac aatcatgctg
     6721 actctgtggc ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc
     6781 ccggcggagt tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag gggcgagatt
     6841 ggagaagcaa taagaatgcc ggttggggtt gcgatgatga cgaccacgac aactggtgtc
     6901 attatttaag ttgccgaaag aacctgagtg catttgcaac atgagtatac tagaagaatg
     6961 agccaagact tgcgagacgc gagtttgccg gtggtgcgaa caatagagcg accatgacct
     7021 tgaaggtgag acgcgcataa ccgctagagt actttgaaga ggaaacagca atagggttgc
     7081 taccagtata aatagacagg tacatacaac actggaaatg gttgtctgtt tgagtacgct
     7141 ttcaattcat ttgggtgtgc actttattat gttacaatat ggaagggaac tttacacttc
     7201 tcctatgcac atatattaat taaagtccaa tgctagtaga gaaggggggt aacacccctc
     7261 cgcgctcttt tccgattttt ttctaaaccg tggaatattt cggatatcct tttgttgttt
     7321 ccgggtgtac aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct
     7381 ataatacctt cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac
     7441 agataccaga caagacataa tgggctaaac aagactacac caattacact gcctcattga
     7501 tg
//
