LOCUS       sequence-387524-        9608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-2 u2 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u2
FEATURES             Location/Qualifiers
     source          1..9608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..36
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        65..466
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             512..532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             548..889
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             902..928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             908..934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2665..2852
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2720..2744)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2873..2906)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2984..3017)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3061..3094)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3172..3205)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3249..3282)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3360..3393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3902..4996)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4997..5402)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5879..7221
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6842..6889)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7486..7590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7591..7659,7660..8451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7809..7828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8622..9210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9111..9130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9364..9381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9396..9536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9462..9481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
       61 gcgaggcagc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
      121 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac
      181 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact
      241 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt
      301 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga
      361 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      421 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      481 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      541 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      601 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      661 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      721 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      781 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      841 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctctatggc
      901 ttacccatac gatgttccag attacgctag cttgggtggt catatggcca tggaggcccc
      961 ggggatctct gccaccatga tcggaggctt attcatctat aatcacaagg gggaggtgct
     1021 tatctcccgg gtctacagag atgacatcgg gaggaatgct gtggatgcct ttcgggtcaa
     1081 tgtcattcat gcacggcagc aggtgcgcag ccctgtcaca aacatcgctc gcaccagctt
     1141 cttccatgtt aagcggtcca acatctggct ggccgcagtc accaagcaga atgtcaatgc
     1201 tgccatggtc ttcgaattcc tctacaagat gtgtgatgta atggctgctt actttggcaa
     1261 aatcagcgag gagaacatca agaacaattt tgtgctcata tacgagctgc tggatgagat
     1321 tctggacttt ggctacccac agaactcaga gacaggtgca ctgaaaacct tcatcaccca
     1381 gcagggtatc aagagtcaga cgaaggaaga acagtcccag atcaccagcc aggtgaccgg
     1441 gcagattggc tggcggcgag aaggcatcaa gtatcgccgg aatgaactct tcctagatgt
     1501 tctggagagt gtgaacctgc ttatgtcccc acaggggcag gtgctgagtg cccatgtgtc
     1561 aggccgggtg gtgatgaaga gttacctgag tggcatgcct gagtgcaagt ttggaatgaa
     1621 tgacaagatt gtcatagaaa agcagggcaa aggcacagct gatgaaacaa gcaagagtgg
     1681 taagcagtcg atcgccattg atgactgcac cttccaccag tgtgtgcgac tcagcaagtt
     1741 tgactctgag cggagcatca gcttcatccc tcccgacgga gagtttgaac tcatgagata
     1801 ccgtactacc aaggacatca tccttccttt ccgggtgatc ccattggtgc gggaggtggg
     1861 gcgcaccaaa ctggaggtca aggtggtcat caagtccaac ttcaagccct cacttctggc
     1921 ccagaagatt gaggtgagga tcccgactcc attgaacaca agcggggtac aggtgatctg
     1981 catgaagggg aaggccaagt acaaggccag cgagaacgcc attgtatgga agatcaagcg
     2041 catggcaggc atgaaggaat cccagatcag cgcagagatt gagctcctgc ccaccaacga
     2101 taagaagaaa tgggctcggc cccccatttc catgaacttt gaggtgccat tcgcgccttc
     2161 tggcctcaag gtgcgctact tgaaggtatt cgaaccgaag ctgaactaca gcgaccatga
     2221 tgtcatcaaa tgggtgcgat acattggccg cagtggcatt tatgaaaccc gctgctagct
     2281 cgagagatct atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc
     2341 gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac
     2401 tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta
     2461 gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct
     2521 tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc
     2581 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga
     2641 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
     2701 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     2761 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     2821 gaggtcgctc ttattgacca cacctctacc ggcatgccgg tcgagggacc taataacttc
     2881 gtatagcata cattatacga agttatatta agggttccgg atcgcggccg ctcgacctgc
     2941 agccaagcta gcttggctgg acgtaaactc ctcttcagac ctaataactt cgtatagcat
     3001 acattatacg aagttatatt aagggttatt gaatatgatc ggaattggtc gagggaccta
     3061 ataacttcgt atagcataca ttatacgaag ttatattaag ggttccggat cgcggccgct
     3121 cgacctgcag ccaagctagc ttggctggac gtaaactcct cttcagacct aataacttcg
     3181 tatagcatac attatacgaa gttatattaa gggttattga atatgatcgg aattggtcga
     3241 gggacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttccggatcg
     3301 cggccgctcg acctgcagcc aagctagctt ggctggacgt aaactcctct tcagacctaa
     3361 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcggaa
     3421 ttggtcgact acgtcgttaa ggccgtttct gacagagtaa aattcttgag ggaactttca
     3481 ccattatggg aaatggttca agaaggtatt gacttaaact ccatcaaatg gtcaggtcat
     3541 tgagtgtttt ttatttgttg tatttttttt tttttagaga aaatcctcca atatataaat
     3601 taggaatcat agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
     3661 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
     3721 aatttgctta cctgtattcc tttacatcct cctttttctc cttcttgata aatgtatgta
     3781 gattgcgtat atagtttcgt ctaccctatg aacatattcc attttgtaat ttcgtgtcgt
     3841 ttctattatg aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt
     3901 tttaagcaag gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt
     3961 tggaaccacc taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt
     4021 caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca
     4081 agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa ccgtggcatg
     4141 gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca
     4201 acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt
     4261 tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag
     4321 aatcaatcaa ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca
     4381 cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag
     4441 gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt
     4501 ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct
     4561 taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa
     4621 attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta
     4681 agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac
     4741 tacctgtacc ccatttagga ccacccacag cacctaacaa aacggcatca gccttcttgg
     4801 aggcttccag cgcctcatct ggaagtggaa cacctgtagc atcgatagca gcaccaccaa
     4861 ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa
     4921 ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct
     4981 tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg
     5041 taaaaggtaa gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat
     5101 aggtccttaa ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg
     5161 cttctctatt ctatatgaaa agccggttcc ggcgctctca cctttccttt ttctcccaat
     5221 ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag
     5281 tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa
     5341 aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag
     5401 ttccttacca ctcttttgtt actctattga tccagctcag caaaggcagt gtgatctaag
     5461 attctatctt cgcgatgtag taaaactagc tagaccgaga aagagactag aaatgcaaaa
     5521 ggcacttcta caatggctgc catcattatt atccgatgtg acgctgcagc ttctcaatga
     5581 tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt tacagattta
     5641 cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga
     5701 aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa
     5761 tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt
     5821 cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata tctttgttaa
     5881 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
     5941 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
     6001 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
     6061 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     6121 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     6181 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     6241 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     6301 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     6361 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     6421 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     6481 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     6541 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     6601 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     6661 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     6721 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg
     6781 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
     6841 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     6901 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     6961 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     7021 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
     7081 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
     7141 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
     7201 catttccttt gatattggat catatgcata gtaccgagaa actagtgcga agtagtgatc
     7261 aggtattgct gttatctgat gagtatacgt tgtcctggcc acggcagaag cacgcttatc
     7321 gctccaattt cccacaacat tagtcaactc cgttaggccc ttcattgaaa gaaatgaggt
     7381 catcaaatgt cttccaatgt gagattttgg gccatttttt atagcaaaga ttgaataagg
     7441 cgcatttttc ttcaaagctc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7801 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8101 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     8161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8281 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8761 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     9421 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     9481 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     9541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     9601 acagacaa
//
