LOCUS       sequence-387525-        9581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-1 u1A 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u1A
FEATURES             Location/Qualifiers
     source          1..9581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..40
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        69..470
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             516..536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             552..893
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             906..932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      1136..1145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      2642..2829
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2697..2721)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2850..2883)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2961..2994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3038..3071)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3149..3182)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3226..3259)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3337..3370)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3879..4973)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4974..5379)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5856..7198
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6819..6866)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7463..7567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7568..7636,7637..8428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7786..7805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8599..9187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9088..9107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9341..9358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9373..9513
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9439..9458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
       61 acgcgcgagg cagcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct
      121 caccatatcc gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa
      181 agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga
      241 aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt
      301 ccttccagtt acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa
      361 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
      421 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact ccaagctttg
      481 caaagatgga taaagcggaa ttaattcccg agcctccaaa aaagaagaga aaggtcgaat
      541 tgggtaccgc cgccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca
      601 ctttcactaa cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc
      661 tttcacaacc aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg
      721 ctagtaaaat tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa
      781 ctgcgtataa cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg
      841 tatataacta tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagatctcta
      901 tggcttaccc atacgatgtt ccagattacg ctagcttggg tggtcatatg gccatggagg
      961 ccccggggat ccgaattccc gccatgtccg ccagcgccgt ctacgtactg gatctgaagg
     1021 gcaaggtgct catctgcagg aactaccgtg gggatgtgga catgtcagag gtggagcact
     1081 tcatgcccat tctgatggag aaggaggagg agggcatgct gtcacctatc ttggcccatg
     1141 gtggcgttcg tttcatgtgg attaagcaca acaacctgta cctggtcgcc acttcaaaaa
     1201 agaatgcttg tgtgtcactg gtgttctcct tcctctacaa ggtggtacag gtcttctccg
     1261 agtactttaa ggagttggag gaggagagca tccgagacaa ctttgtcatc atctacgagc
     1321 tgctagatga gctcatggac tttggctacc cgcagaccac tgacagcaag atcttgcagg
     1381 agtacatcac tcaggaaggc cacaagctgg aaacgggggc ccctaggccc ccagccacag
     1441 tcaccaatgc tgtgtcctgg cgttcagaag gcatcaagta tcggaagaat gaagtattcc
     1501 tggatgtcat tgaggctgtt aacctcttgg tcagtgccaa tggcaacgtg ctgcgcagtg
     1561 agattgtggg ttccatcaag atgcgggtct tcctctcagg catgcctgag ttacgcctgg
     1621 gtctcaatga caaggtcctc ttcgacaaca caggccgagg gaagagcaag tcagtggagc
     1681 tggaggatgt gaaattccac cagtgtgtgc ggctgtcacg ttttgagaac gaccgcacta
     1741 tctccttcat cccacccgac ggagagtttg aactcatgtc ctaccgcctc aacacccatg
     1801 tgaagccttt gatctggatt gagtccgtga ttgagaagca ttcccacagc cgcattgagt
     1861 acatggtcaa ggccaagagc cagttcaaga ggcggtcaac agccaacaat gtagagatcc
     1921 atataccagt ccccaacgat gctgattcac ccaagttcaa gactacagtg gggagtgtca
     1981 agtgggtccc tgaaaacagt gagatcgtgt ggtccgtcaa gtcctttccg ggtggcaagg
     2041 agtacctgat gcgggcccac tttggccttc ccagtgtgga agctgaagac aaggagggaa
     2101 agccccccat cagcgtcaag tttgagatcc cctacttcac tacctctggc atccaggtgc
     2161 gctacctgaa aatcattgag aagagtgggt accaggccct gccctgggta cgatatatca
     2221 cacagaacgg agattatcag ctccggaccc agtgactcga gagatctatg aatcgtagat
     2281 actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg caccatctca atttctttca
     2341 tttatacatc gttttgcctt cttttatgta actatactcc tctaagtttc aatcttggcc
     2401 atgtaacctc tgatctatag aattttttaa atgactagaa ttaatgccca tctttttttt
     2461 ggacctaaat tcttcatgaa aatatattac gagggcttat tcagaagctt tggacttctt
     2521 cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat
     2581 ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca cttctaaata
     2641 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     2701 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     2761 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     2821 ctctaccggc atgccggtcg agggacctaa taacttcgta tagcatacat tatacgaagt
     2881 tatattaagg gttccggatc gcggccgctc gacctgcagc caagctagct tggctggacg
     2941 taaactcctc ttcagaccta ataacttcgt atagcataca ttatacgaag ttatattaag
     3001 ggttattgaa tatgatcgga attggtcgag ggacctaata acttcgtata gcatacatta
     3061 tacgaagtta tattaagggt tccggatcgc ggccgctcga cctgcagcca agctagcttg
     3121 gctggacgta aactcctctt cagacctaat aacttcgtat agcatacatt atacgaagtt
     3181 atattaaggg ttattgaata tgatcggaat tggtcgaggg acctaataac ttcgtatagc
     3241 atacattata cgaagttata ttaagggttc cggatcgcgg ccgctcgacc tgcagccaag
     3301 ctagcttggc tggacgtaaa ctcctcttca gacctaataa cttcgtatag catacattat
     3361 acgaagttat attaagggtt attgaatatg atcggaattg gtcgactacg tcgttaaggc
     3421 cgtttctgac agagtaaaat tcttgaggga actttcacca ttatgggaaa tggttcaaga
     3481 aggtattgac ttaaactcca tcaaatggtc aggtcattga gtgtttttta tttgttgtat
     3541 tttttttttt ttagagaaaa tcctccaata tataaattag gaatcatagt ttcatgattt
     3601 tctgttacac ctaacttttt gtgtggtgcc ctcctccttg tcaatattaa tgttaaagtg
     3661 caattctttt tccttatcac gttgagccat tagtatcaat ttgcttacct gtattccttt
     3721 acatcctcct ttttctcctt cttgataaat gtatgtagat tgcgtatata gtttcgtcta
     3781 ccctatgaac atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata
     3841 aagtttatgt acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact
     3901 tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt
     3961 ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc
     4021 aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc
     4081 ttattctttg gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg
     4141 ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata
     4201 acggaggctt catcggagat gatatcacca aacatgttgc tggtgattat aataccattt
     4261 aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc
     4321 ttcaatgtag gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa
     4381 gaggccaaaa cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg
     4441 gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta
     4501 tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact
     4561 aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag
     4621 tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct
     4681 ttacggattt ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca
     4741 cccacagcac ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc ctcatctgga
     4801 agtggaacac ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac
     4861 ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt
     4921 tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg
     4981 gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
     5041 gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
     5101 cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
     5161 cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg
     5221 cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga
     5281 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg
     5341 aactcttgca tcttacgata cctgagtatt cccacagttc cttaccactc ttttgttact
     5401 ctattgatcc agctcagcaa aggcagtgtg atctaagatt ctatcttcgc gatgtagtaa
     5461 aactagctag accgagaaag agactagaaa tgcaaaaggc acttctacaa tggctgccat
     5521 cattattatc cgatgtgacg ctgcagcttc tcaatgatat tcgaatacgc tttgaggaga
     5581 tacagcctaa tatccgacaa actgttttac agatttacga tcgtacttgt tacccatcat
     5641 tgaattttga acatccgaac ctgggagttt tccctgaaac agatagtata tttgaacctg
     5701 tataataata tatagtctag cgctttacgg aagacaatgt atgtatttcg gttcctggag
     5761 aaactattgc atctattgca taggtaatct tgcacgtcgc atccccggtt cattttctgc
     5821 gtttccatct tgcacttcaa tagcatatct ttgttaacga agcatctgtg cttcattttg
     5881 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     5941 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     6001 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     6061 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     6121 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     6181 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     6241 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     6301 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     6361 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     6421 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     6481 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     6541 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     6601 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     6661 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     6721 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     6781 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     6841 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     6901 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     6961 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     7021 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     7081 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     7141 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     7201 atgcatagta ccgagaaact agtgcgaagt agtgatcagg tattgctgtt atctgatgag
     7261 tatacgttgt cctggccacg gcagaagcac gcttatcgct ccaatttccc acaacattag
     7321 tcaactccgt taggcccttc attgaaagaa atgaggtcat caaatgtctt ccaatgtgag
     7381 attttgggcc attttttata gcaaagattg aataaggcgc atttttcttc aaagctcagg
     7441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7801 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8101 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8401 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8701 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     8761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9241 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9301 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9361 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     9421 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     9481 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     9541 gcgccctgac gggcttgtct gctcccggca tccgcttaca g
//
