LOCUS       sequence-387528-       11012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutated mir21 binding sites (3x) were integrated in between the stop
            codon and TREAT cassette in IRE-TREAT reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: miR21mutant-IRE-TREAT
FEATURES             Location/Qualifiers
     source          1..11012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4086..4110
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4330..5265
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8810..8944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8888..8907
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9052..9085)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9138..9154)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9170..9192)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9603..9960
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10025..10494
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataaag ctttaggcgt gtacggtggg cgcctataaa
     4081 agcagagctc gtttagtgaa ccgtcagatc gcctgcttcc agacgttctt cgccgagagt
     4141 cgtcggggtt tcctgcttca acagtgcttg gacggaaccc ggcgctcgtt ccccaccccg
     4201 gccggccgcc catagccagc cctccgtcac ctcttcaccg caccctcgga ctgccccaag
     4261 gcccccgccg ccgctccagc gccgcgcagc caccgccgcc gccgccgcct ctccttagtc
     4321 gccgccatga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     4381 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     4441 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     4501 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     4561 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     4621 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     4681 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     4741 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     4801 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     4861 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     4921 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     4981 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     5041 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     5101 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     5161 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     5221 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaagggcc agggaagaac
     5281 tgatcgccgt gtaaccgcgg agttgtagtc agactattcg aatcggaagt tgtagtcaga
     5341 ctattcgaag gcctagttgt agtcagacta ttcgaaccgc ggcgcggcgg gatccggatc
     5401 caacctacaa aagatcctac ctgcaaagca ccagaggata tggcctcggt gcctcagcat
     5461 cttcagatgt gctgtcactg tccaatcttc aagccagcag agcatatggg ctcgctggct
     5521 ccactgtcat tacagtggac ctgatcttct ccaatcaaag gagcagagga tatggcctcg
     5581 ctccctccga ctacatcagg ctggaagtga catccatcat tcatcccagc agagcatatg
     5641 ggctcgctgg gaccacagca tgcctcgaag tgctgagaga ctatcatcaa accgacagag
     5701 gatatggcct cgtcggctcc tactccctcc tggtggaata cttctccact cttcaaccca
     5761 gcagagcata tgggctcgct gggtccatca gagatacaag atctctcgac agatcctacc
     5821 tgcaaagcac cagaggatat ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt
     5881 ccaatcttca agccagcaga gcatatgggc tcgctggctc cactgtcatt acagtggacc
     5941 tgatcttctc caatcaaagg agcagaggat atggcctcgc tccctccgac tacatcaggc
     6001 tggaagtgac atccatcatt catcccagca gagcatatgg gctcgctggg accacagcat
     6061 gcctcgaagt gctgagagac tatcatcaaa ccgacagagg atatggcctc gtcggctcct
     6121 actccctcct ggtggaatac ttctccactc ttcaacccag cagagcatat gggctcgctg
     6181 ggtccatcag agatacaaga tctctcgaca gatcctacct gcaaagcacc agaggatatg
     6241 gcctcggtgc ctcagcatct tcagatgtgc tgtcactgtc caatcttcaa gccagcagag
     6301 catatgggct cgctggctcc actgtcatta cagtggacct gatcttctcc aatcaaagga
     6361 gcagaggata tggcctcgct ccctccgact acatcaggct ggaagtgaca tccatcattc
     6421 atcccagcag agcatatggg ctcgctggga ccacagcatg cctcgaagtg ctgagagact
     6481 atcatcaaac cgacagagga tatggcctcg tcggctccta ctccctcctg gtggaatact
     6541 tctccactct tcaacccagc agagcatatg ggctcgctgg gtccatcaga gatacaagat
     6601 ctctcgacag atcctacctg caaagcacca gaggatatgg cctcggtgcc tcagcatctt
     6661 cagatgtgct gtcactgtcc aatcttcaag ccagcagagc atatgggctc gctggctcca
     6721 ctgtcattac agtggacctg atcttctcca atcaaaggag cagaggatat ggcctcgctc
     6781 cctccgacta catcaggctg gaagtgacat ccatcattca tcccagcaga gcatatgggc
     6841 tcgctgggac cacagcatgc ctcgaagtgc tgagagacta tcatcaaacc gacagaggat
     6901 atggcctcgt cggctcctac tccctcctgg tggaatactt ctccactctt caacccagca
     6961 gagcatatgg gctcgctggg tccatcagag atacaagatc tctcgagccg cggtgcgtaa
     7021 gtctgttaac agaatgctgc atacgctgag tacttcgaaa tgtcatcctc tgtctgacac
     7081 tgaacgtaat ccagacgcgt aagtcaggcc ggaaaattcc cgccaccgga agttgagtag
     7141 acggtgctgc ctgcgactca accccaggag gactgggtga acaaagctgc gaagtgatcc
     7201 atgtaagccc tcagaaccgt ctcggaaaga ggaccccaca tgttgtagct tcaaggccca
     7261 atgtcagacc acgccatggc gtgccactct gcggagagtg cagtctgcga cagtgcccca
     7321 ggaggactgg gtgaggatcc tacctacaaa cggcacgagc atcagccgtg ccgcccagca
     7381 tcttcatcac tgtccaatct tcaagcgtgc acgatcacgg cacgctccac tgtcattaca
     7441 gtcttatcca atcaaactcc agcagcatca gcgctggagc tccgactaca tcaggacatc
     7501 catcattcaa cgctcgacga tcacgcgagc gtccacagca tgcctcagac actatcatca
     7561 aacggctgca cgatcacggc agccgctcct actgcctcta cgttatcgac tcttctgcga
     7621 cgagcatcag ccgtcgcaac atcggacata ccagatccta cctacaaacg gcacgagcat
     7681 cagccgtgcc gcccagcatc ttcatcactg tccaatcttc aagcgtgcac gatcacggca
     7741 cgctccactg tcattacagt cttatccaat caaactccag cagcatcagc gctggagctc
     7801 cgactacatc aggacatcca tcattcaacg ctcgacgatc acgcgagcgt ccacagcatg
     7861 cctcagacac tatcatcaaa cggctgcacg atcacggcag ccgctcctac tgcctctacg
     7921 ttatcgactc ttctgcgacg agcatcagcc gtcgcaacat cggacatacc agatcctacc
     7981 tacaaacggc acgagcatca gccgtgccgc ccagcatctt catcactgtc caatcttcaa
     8041 gcgtgcacga tcacggcacg ctccactgtc attacagtct tatccaatca aactccagca
     8101 gcatcagcgc tggagctccg actacatcag gacatccatc attcaacgct cgacgatcac
     8161 gcgagcgtcc acagcatgcc tcagacacta tcatcaaacg gctgcacgat cacggcagcc
     8221 gctcctactg cctctacgtt atcgactctt ctgcgacgag catcagccgt cgcaacatcg
     8281 gacataccag atcctaccta caaacggcac gagcatcagc cgtgccgccc agcatcttca
     8341 tcactgtcca atcttcaagc gtgcacgatc acggcacgct ccactgtcat tacagtctta
     8401 tccaatcaaa ctccagcagc atcagcgctg gagctccgac tacatcagga catccatcat
     8461 tcaacgctcg acgatcacgc gagcgtccac agcatgcctc agacactatc atcaaacggc
     8521 tgcacgatca cggcagccgc tcctactgcc tctacgttat cgactcttct gcgacgagca
     8581 tcagccgtcg caacatcgga cataccagat cttcaatcga tttcgcgcgg gatccagacc
     8641 acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac atttttcact
     8701 aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt aaaagtgata
     8761 aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgag atccagacat
     8821 gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct
     8881 tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca
     8941 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     9001 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tgaagttcct
     9061 atactatttg aagaatagga acttcggaat aggaacttcg gatctcatat ggccatatgg
     9121 gggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
     9181 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
     9241 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
     9301 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
     9361 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
     9421 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
     9481 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca ggctagcccg
     9541 ggcccatatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc
     9601 tgcagcagac aagacgcgcg gcgcggcttc ggtcccaaac cgaaagcaaa aattcagacg
     9661 gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatccc tcctctaagg
     9721 ggggtgcacc aaagagtcct aaacgatcgg gattttagga ctcaggtcgg gccacaaaaa
     9781 cggcccccga agtccctggg acgtctccca gggttgcggc cgggtgttcc gaactcgtca
     9841 gttccaccac gggtccgcca gatacagagc tagttagcta actagtaccg acgcaggcgc
     9901 ataaaatcag tcatagacac tagacaatcg gacagacaca gataagttgc tggccagctt
     9961 acctcccggt ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg gacgagcccc
    10021 caaatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
    10081 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
    10141 cgactcagtc aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag
    10201 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
    10261 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa
    10321 actgctgagg gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg
    10381 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc
    10441 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc
    10501 catctgttcc tgaccttgat ctgaactttt ctattctcag ttatgtattt ttccatgcct
    10561 tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcacatgta
    10621 tatgtcaaaa ataaaaatca actaattgac tagtaattaa tatgactggc ataatgggaa
    10681 attgatcctg acagatgcaa actggcttct cagcagcgca tttatgttgt caactgagga
    10741 aggaaacgtt aatgacagaa actctaagta atttccacgt ttatctattt ttatttatac
    10801 tagctttggt aacaggaata ttgcagcatt catgcacatt gaaaccctta tgaaataaaa
    10861 acatctgtgc atttaaaatg gaattaacat tttaaatgtt aaaaaaagct ggcttagctt
    10921 ccccccgccc cctagggcat agaacaagtc aaatgcttta tatatttgag tttgggatgt
    10981 attaggaaac tcctaagagc aaagctagct tg
//
