LOCUS       sequence-387529-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The TREAT reporter containing cap-proximal GC-rich stemloop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
  TITLE     Single-molecule imaging reveals translation-dependent 
            destabilization of mRNAs.
  JOURNAL   Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 
            10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
  PUBMED    36731471
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.013"; journalName: "Mol Cell"; date: 
            "2023-02-16- 16"; volume: "83"; issue: "4"; pages: "589-606.e6"
COMMENT     Sequence Label: SL-TREAT
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          4207..4339
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4322..4341
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4384..4402
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4406..4415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4412..5347
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    7954..8088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8032..8051
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8196..8229)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8282..8298)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(8314..8336)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8747..9104
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9169..9638
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     4081 gcagagctcg tttagtgaac cgtcagatgc gccggtgcag ccggcgcagc gcctggagca
     4141 attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaaacc
     4201 ggtcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     4261 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     4321 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     4381 acttaatacg actcactata ggctagccac catggcttcc aaggtgtacg accccgagca
     4441 acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct
     4501 ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg tgatttttct
     4561 gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt
     4621 ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga gcgggaatgg
     4681 ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc tgctgaacct
     4741 tccaaagaaa atcatctttg tgggccacga ctggggggct tgtctggcct ttcactactc
     4801 ctacgagcac caagacaaga tcaaggccat cgtccatgct gagagtgtcg tggacgtgat
     4861 cgagtcctgg gacgagtggc ctgacatcga ggaggatatc gccctgatca agagcgaaga
     4921 gggcgagaaa atggtgcttg agaataactt cttcgtcgag accatgctcc caagcaagat
     4981 catgcggaaa ctggagcctg aggagttcgc tgcctacctg gagccattca aggagaaggg
     5041 cgaggttaga cggcctaccc tctcctggcc tcgcgagatc cctctcgtta agggaggcaa
     5101 gcccgacgtc gtccagattg tccgcaacta caacgcctac cttcgggcca gcgacgatct
     5161 gcctaagatg ttcatcgagt ccgaccctgg gttcttttcc aacgctattg tcgagggagc
     5221 taagaagttc cctaacaccg agttcgtgaa ggtgaagggc ctccacttca gccaggagga
     5281 cgctccagat gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga
     5341 gcagtaagtc gacagatcct acctgcaaag caccagagga tatggcctcg gtgcctcagc
     5401 atcttcagat gtgctgtcac tgtccaatct tcaagccagc agagcatatg ggctcgctgg
     5461 ctccactgtc attacagtgg acctgatctt ctccaatcaa aggagcagag gatatggcct
     5521 cgctccctcc gactacatca ggctggaagt gacatccatc attcatccca gcagagcata
     5581 tgggctcgct gggaccacag catgcctcga agtgctgaga gactatcatc aaaccgacag
     5641 aggatatggc ctcgtcggct cctactccct cctggtggaa tacttctcca ctcttcaacc
     5701 cagcagagca tatgggctcg ctgggtccat cagagataca agatctctcg acagatccta
     5761 cctgcaaagc accagaggat atggcctcgg tgcctcagca tcttcagatg tgctgtcact
     5821 gtccaatctt caagccagca gagcatatgg gctcgctggc tccactgtca ttacagtgga
     5881 cctgatcttc tccaatcaaa ggagcagagg atatggcctc gctccctccg actacatcag
     5941 gctggaagtg acatccatca ttcatcccag cagagcatat gggctcgctg ggaccacagc
     6001 atgcctcgaa gtgctgagag actatcatca aaccgacaga ggatatggcc tcgtcggctc
     6061 ctactccctc ctggtggaat acttctccac tcttcaaccc agcagagcat atgggctcgc
     6121 tgggtccatc agagatacaa gatctctcga gccgcggtgc gtaagtctgt taacagaatg
     6181 ctgcatacgc tgagtacttc gaaatgtcat cctctgtctg acactgaacg taatccagac
     6241 gcgtaagtca ggccggaaaa ttcccgccac cggaagttga gtagacggtg ctgcctgcga
     6301 ctcaacccca ggaggactgg gtgaacaaag ctgcgaagtg atccatgtaa gccctcagaa
     6361 ccgtctcgga aagaggaccc cacatgttgt agcttcaagg cccaatgtca gaccacgcca
     6421 tggcgtgcca ctctgcggag agtgcagtct gcgacagtgc cccaggagga ctgggtgagg
     6481 atcctaccta caaacggcac gagcatcagc cgtgccgccc agcatcttca tcactgtcca
     6541 atcttcaagc gtgcacgatc acggcacgct ccactgtcat tacagtctta tccaatcaaa
     6601 ctccagcagc atcagcgctg gagctccgac tacatcagga catccatcat tcaacgctcg
     6661 acgatcacgc gagcgtccac agcatgcctc agacactatc atcaaacggc tgcacgatca
     6721 cggcagccgc tcctactgcc tctacgttat cgactcttct gcgacgagca tcagccgtcg
     6781 caacatcgga cataccagat cctacctaca aacggcacga gcatcagccg tgccgcccag
     6841 catcttcatc actgtccaat cttcaagcgt gcacgatcac ggcacgctcc actgtcatta
     6901 cagtcttatc caatcaaact ccagcagcat cagcgctgga gctccgacta catcaggaca
     6961 tccatcattc aacgctcgac gatcacgcga gcgtccacag catgcctcag acactatcat
     7021 caaacggctg cacgatcacg gcagccgctc ctactgcctc tacgttatcg actcttctgc
     7081 gacgagcatc agccgtcgca acatcggaca taccagatcc tacctacaaa cggcacgagc
     7141 atcagccgtg ccgcccagca tcttcatcac tgtccaatct tcaagcgtgc acgatcacgg
     7201 cacgctccac tgtcattaca gtcttatcca atcaaactcc agcagcatca gcgctggagc
     7261 tccgactaca tcaggacatc catcattcaa cgctcgacga tcacgcgagc gtccacagca
     7321 tgcctcagac actatcatca aacggctgca cgatcacggc agccgctcct actgcctcta
     7381 cgttatcgac tcttctgcga cgagcatcag ccgtcgcaac atcggacata ccagatccta
     7441 cctacaaacg gcacgagcat cagccgtgcc gcccagcatc ttcatcactg tccaatcttc
     7501 aagcgtgcac gatcacggca cgctccactg tcattacagt cttatccaat caaactccag
     7561 cagcatcagc gctggagctc cgactacatc aggacatcca tcattcaacg ctcgacgatc
     7621 acgcgagcgt ccacagcatg cctcagacac tatcatcaaa cggctgcacg atcacggcag
     7681 ccgctcctac tgcctctacg ttatcgactc ttctgcgacg agcatcagcc gtcgcaacat
     7741 cggacatacc agatcttcaa tcgatttcgc gcgggatcca gaccacctcc cctgcgagct
     7801 aagctggaca gccaatgacg ggtaagagag tgacattttt cactaaccta agacaggagg
     7861 gccgtcagag ctactgccta atccaaagac gggtaaaagt gataaaaatg tatcactcca
     7921 acctaagaca ggcgcagctt ccgagggatt tgagatccag acatgataag atacattgat
     7981 gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat gccttatttg tgaaatttgt
     8041 gatgctattg ccttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     8101 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     8161 aacctctaca aatgtggtat ggctgattat gatctgaagt tcctatacta tttgaagaat
     8221 aggaacttcg gaataggaac ttcggatctc atatggccat atgggggcgc ctagagaagg
     8281 agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag
     8341 agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca
     8401 gggagggggg tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg
     8461 tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa
     8521 ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac
     8581 ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca
     8641 gtgacctaag gtcaaactta agggagtggt aacaggctag cccgggccca tattttcaga
     8701 caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc agacaagacg
     8761 cgcggcgcgg cttcggtccc aaaccgaaag caaaaattca gacggaggcg ggaactgttt
     8821 taggttctcg tctcctacca gaaccacata tccctcctct aaggggggtg caccaaagag
     8881 tcctaaacga tcgggatttt aggactcagg tcgggccaca aaaacggccc ccgaagtccc
     8941 tgggacgtct cccagggttg cggccgggtg ttccgaactc gtcagttcca ccacgggtcc
     9001 gccagataca gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag
     9061 acactagaca atcggacaga cacagataag ttgctggcca gcttacctcc cggtggtggg
     9121 tcggtggtcc ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc
     9181 gctgacgggt agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc
     9241 ggatgcaact gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg
     9301 aggactggcg cgccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc
     9361 gcgcgaacag aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt
     9421 caggtccttg gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga
     9481 ccgcatctgg ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga
     9541 tatcctgttt ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga
     9601 actgcttacc acagatatcc tgtttggccc atattcagct gttccatctg ttcctgacct
     9661 tgatctgaac ttttctattc tcagttatgt atttttccat gccttgcaaa atggcgttac
     9721 ttaagctagc ttgccaaacc tacaggtggg gtctttcaca tgtatatgtc aaaaataaaa
     9781 atcaactaat tgactagtaa ttaatatgac tggcataatg ggaaattgat cctgacagat
     9841 gcaaactggc ttctcagcag cgcatttatg ttgtcaactg aggaaggaaa cgttaatgac
     9901 agaaactcta agtaatttcc acgtttatct atttttattt atactagctt tggtaacagg
     9961 aatattgcag cattcatgca cattgaaacc cttatgaaat aaaaacatct gtgcatttaa
    10021 aatggaatta acattttaaa tgttaaaaaa agctggctta gcttcccccc gccccctagg
    10081 gcatagaaca agtcaaatgc tttatatatt tgagtttggg atgtattagg aaactcctaa
    10141 gagcaaagct agcttg
//
