LOCUS       sequence-387535-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-1 u1B 
            (137-423) fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u1B (137-423)
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..38
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        67..468
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             514..534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             550..891
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             904..930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             910..936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2230..2417
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2285..2309)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2438..2471)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2549..2582)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2626..2659)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2737..2770)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2814..2847)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2925..2958)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3467..4561)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4562..4967)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5444..6786
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6407..6454)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7051..7155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7156..7224,7225..8016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7374..7393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8187..8775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8676..8695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8929..8946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8961..9101
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9027..9046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
       61 gcgcgaggca gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca
      121 ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag
      181 acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa
      241 ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat acggccttcc
      301 ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca agtataaata
      361 gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt
      421 ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc aagctttgca
      481 aagatggata aagcggaatt aattcccgag cctccaaaaa agaagagaaa ggtcgaattg
      541 ggtaccgccg ccaattttaa tcaaagtggg aatattgctg atagctcatt gtccttcact
      601 ttcactaaca gtagcaacgg tccgaacctc ataacaactc aaacaaattc tcaagcgctt
      661 tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct
      721 agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact
      781 gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta
      841 tataactatc tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gatctctatg
      901 gcttacccat acgatgttcc agattacgct agcttgggtg gtcatatggc catggaggcc
      961 ccggggatcc gaattcgaca gcagagcaac aagctggaga cgggcaagtc acgggtgcca
     1021 cccactgtca ccaacgctgt gtcctggcgc tccgagggta tcaagtataa gaagaacgag
     1081 gtcttcattg atgtcataga gtctgtcaac ctgctggtca atgccaacgg cagcgtcctt
     1141 ctgagcgaaa tcgtcggtac catcaagctc aaggtgtttc tgtcaggaat gccagagctg
     1201 cggctgggcc tcaatgaccg cgtgctcttc gagctcactg gccgcagcaa gaacaaatca
     1261 gtagagctgg aggatgtaaa attccaccag tgcgtgcggc tctctcgctt tgacaacgac
     1321 cgcaccatct ccttcatccc gcctgatggt gactttgagc tcatgtcata ccgcctcagc
     1381 acccaggtca agccactgat ctggattgag tctgtcattg agaagttctc ccacagccgc
     1441 gtggagatca tggtcaaggc caaggggcag tttaagaaac agtcagtggc caacggtgtg
     1501 gagatatctg tgcctgtacc cagcgatgcc gactccccca gattcaagac cagtgtgggc
     1561 agcgccaagt atgtgccgga gagaaacgtc gtgatttgga gtattaagtc tttcccgggg
     1621 ggcaaggagt acttgatgcg agcccacttt ggcctcccca gtgtggaaaa ggaagaggtg
     1681 gagggccggc cccccatcgg ggtcaagttt gagatcccct acttcaccgt ctctgggatc
     1741 caggtccgat acatgaagat cattgagaaa agtggttacc aggccctgcc ctgggttcgc
     1801 tacatcaccc agagtggcga ttaccaactt cgtaccagct agactcgaga gatctatgaa
     1861 tcgtagatac tgaaaaaccc cgcaagttca cttcaactgt gcatcgtgca ccatctcaat
     1921 ttctttcatt tatacatcgt tttgccttct tttatgtaac tatactcctc taagtttcaa
     1981 tcttggccat gtaacctctg atctatagaa ttttttaaat gactagaatt aatgcccatc
     2041 ttttttttgg acctaaattc ttcatgaaaa tatattacga gggcttattc agaagctttg
     2101 gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg
     2161 ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact
     2221 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
     2281 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
     2341 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
     2401 gaccacacct ctaccggcat gccggtcgag ggacctaata acttcgtata gcatacatta
     2461 tacgaagtta tattaagggt tccggatcgc ggccgctcga cctgcagcca agctagcttg
     2521 gctggacgta aactcctctt cagacctaat aacttcgtat agcatacatt atacgaagtt
     2581 atattaaggg ttattgaata tgatcggaat tggtcgaggg acctaataac ttcgtatagc
     2641 atacattata cgaagttata ttaagggttc cggatcgcgg ccgctcgacc tgcagccaag
     2701 ctagcttggc tggacgtaaa ctcctcttca gacctaataa cttcgtatag catacattat
     2761 acgaagttat attaagggtt attgaatatg atcggaattg gtcgagggac ctaataactt
     2821 cgtatagcat acattatacg aagttatatt aagggttccg gatcgcggcc gctcgacctg
     2881 cagccaagct agcttggctg gacgtaaact cctcttcaga cctaataact tcgtatagca
     2941 tacattatac gaagttatat taagggttat tgaatatgat cggaattggt cgactacgtc
     3001 gttaaggccg tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg
     3061 gttcaagaag gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt
     3121 tgttgtattt tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt
     3181 catgattttc tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg
     3241 ttaaagtgca attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt
     3301 attcctttac atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt
     3361 ttcgtctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt
     3421 catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt
     3481 tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat
     3541 caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt
     3601 cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag
     3661 ggttgacctt attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa
     3721 atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac
     3781 ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa
     3841 taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat
     3901 gttgaacctt caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata
     3961 atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat
     4021 gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt
     4081 gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac
     4141 ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg
     4201 gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt
     4261 gaagttcttt acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt
     4321 taggaccacc cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct
     4381 catctggaag tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga
     4441 aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg
     4501 ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca
     4561 ttagaatggt atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa
     4621 gttagaaagt aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat
     4681 attgtcaact tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata
     4741 tgaaaagccg gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa
     4801 ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt
     4861 ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa
     4921 gagattcgaa ctcttgcatc ttacgatacc tgagtattcc cacagttcct taccactctt
     4981 ttgttactct attgatccag ctcagcaaag gcagtgtgat ctaagattct atcttcgcga
     5041 tgtagtaaaa ctagctagac cgagaaagag actagaaatg caaaaggcac ttctacaatg
     5101 gctgccatca ttattatccg atgtgacgct gcagcttctc aatgatattc gaatacgctt
     5161 tgaggagata cagcctaata tccgacaaac tgttttacag atttacgatc gtacttgtta
     5221 cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt
     5281 tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt
     5341 tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca
     5401 ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct
     5461 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     5521 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
     5581 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     5641 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
     5701 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
     5761 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
     5821 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
     5881 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
     5941 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
     6001 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
     6061 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
     6121 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
     6181 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
     6241 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
     6301 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
     6361 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
     6421 ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     6481 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     6541 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     6601 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
     6661 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
     6721 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
     6781 tggatcatat gcatagtacc gagaaactag tgcgaagtag tgatcaggta ttgctgttat
     6841 ctgatgagta tacgttgtcc tggccacggc agaagcacgc ttatcgctcc aatttcccac
     6901 aacattagtc aactccgtta ggcccttcat tgaaagaaat gaggtcatca aatgtcttcc
     6961 aatgtgagat tttgggccat tttttatagc aaagattgaa taaggcgcat ttttcttcaa
     7021 agctcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7381 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     7441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7681 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7861 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8821 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8881 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8941 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9001 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9061 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     9121 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga c
//
